2020
DOI: 10.1038/s41598-020-71070-2
|View full text |Cite
|
Sign up to set email alerts
|

The bHLH transcription factor VfTT8 underlies zt2, the locus determining zero tannin content in faba bean (Vicia faba L.)

Abstract: faba bean (Vicia faba L.) is an important protein-rich fodder crop, which is widely cultivated in temperate areas. However, antinutritional compounds such as condensed tannins, limit the use of this protein source in monogastric feed formulations. Previous studies demonstrated that two recessive and complementary genes, zt1 and zt2, control absence of tannin and white flower colour in faba bean. An ortholog of the Medicago WD40 transcription factor TTG1 was reported to encode the zt1 phenotype, but the respons… Show more

Help me understand this report
View preprint versions

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

0
18
0

Year Published

2020
2020
2023
2023

Publication Types

Select...
3
3
1

Relationship

0
7

Authors

Journals

citations
Cited by 19 publications
(18 citation statements)
references
References 42 publications
0
18
0
Order By: Relevance
“…By deep learning of genotyping‐by‐sequencing (GBS) data of faba bean lines with high and low V–C content, two regulatory SNPs (rSNPs) were identified to be significantly associated with V–C content (Heinrich et al, 2020). In a recent study, mutations in Transparent Testa Glabra 1 ( TVfTG1 ), encoding a WD40 transcription factor, and Transparent Testa 8 ( VfTT8 , Vf_Mt1g072320 ) encoding a bHLH domain transcription factor, were reported to be responsible for zero tannin and white flower color in faba bean (Gutierrez et al, 2020; Gutierrez & Torres, 2019). However, further studies are required to elucidate the regulatory mechanism of these candidate genes and their influence on other biological processes and metabolites.…”
Section: Crispr/cas9 For Crop Improvement In Legumesmentioning
confidence: 99%
“…By deep learning of genotyping‐by‐sequencing (GBS) data of faba bean lines with high and low V–C content, two regulatory SNPs (rSNPs) were identified to be significantly associated with V–C content (Heinrich et al, 2020). In a recent study, mutations in Transparent Testa Glabra 1 ( TVfTG1 ), encoding a WD40 transcription factor, and Transparent Testa 8 ( VfTT8 , Vf_Mt1g072320 ) encoding a bHLH domain transcription factor, were reported to be responsible for zero tannin and white flower color in faba bean (Gutierrez et al, 2020; Gutierrez & Torres, 2019). However, further studies are required to elucidate the regulatory mechanism of these candidate genes and their influence on other biological processes and metabolites.…”
Section: Crispr/cas9 For Crop Improvement In Legumesmentioning
confidence: 99%
“…Very recently, the biosynthetic pathway of v-c was uncovered (Björnsdotter et al, 2021). Similarly, robust DNA markers for low seed coat tannins zt1 (Webb et al, 2016;Gutierrez and Torres, 2019) and zt2 (Gutierrez et al, 2020;Zanotto et al, 2020), growth habit (Vf_TFL1, Avila et al, 2006Avila et al, , 2007 and rust resistance genes (Uvf1 and Uvf2, Ijaz et al, 2021b) have been developed. Furthermore, closely linked DNA markers have been reported for host plant resistance to ascochyta blight (Avila et al, 2004;Kaur et al, 2014;Atienza et al, 2016;Sudheesh et al, 2019;Faridi et al, 2021), however, their verification and availability in breeder friendly format is yet to come.…”
Section: Marker-assisted Selection and Dna Marker Availabilitymentioning
confidence: 99%
“…The absence of an annotated reference genome for this crop poses challenges for the application of CRISPR/Cas gene editing, particularly with the design of specific gRNA-targeted genes of interest (Bhowmik et al, 2021). However, with recent gene discoveries for quality traits such as vicine-convicine (v-c) (Björnsdotter et al, 2021) and seed coat tannins (Gutierrez and Torres, 2019;Gutierrez et al, 2020), this technology may be used to advance our understanding of gene function and accelerate development of new cultivars with reduced anti-nutritional factors. The aim of this review is to highlight species-specific advantages, restrictions and limitations, progress made to date in applied genetics and crossbreeding and what needs to be done to markedly accelerate genetic gains in faba bean.…”
Section: Introductionmentioning
confidence: 99%
“…An allele-specific diagnostic marker was also developed that differentiates zt1 from other genotypes. Gutierrez et al (2020) reported the bHLH transcription factor VfTT8 (Transparent Testa8) located on chromosome 3 as the zt2 gene. A robust KASP marker for the zt2 gene is now available (Zanotto et al, 2020).…”
Section: Successful Gene Discoveries In the Absence Of A Faba Bean Rementioning
confidence: 99%
“…Two recent review papers on this topic cover the coming of age of faba bean genetics and genomics in some detail (see O'Sullivan & Angra, 2016;Maalouf et al, 2019) but, since then, major progress on the key seed anti-nutrients v-c (Björnsdotter et al, 2020), seed coat tannins (e.g., Gutierrez & Torres, 2019;Gutierrez et al, 2020), as well as improved mapping approaches (Carrillo-Perdomo et al, 2020) and transcriptome data, has been made. We provide here a comprehensive review on the mapping population and genomic resources in this species.…”
mentioning
confidence: 99%