The bile salt content of human bile impacts on simulated intestinal proteolysis of β-lactoglobulin

Dulko, Dorota; Staroń, Robert; Krupa, Łukasz; Rigby, Neil M.; Mackie, Alan; Gutkowski, Krzysztof; Wasik, Andrzej; Macierzanka, Adam

doi:10.1016/j.foodres.2021.110413

Cited by 11 publications

(4 citation statements)

References 45 publications

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“…As shown in SDS-PAGE (Figure 5d), the digestion dynamics of 7S aggregates with pancreatic enzymes are closely correlated with the cmc of BS, which is in agreement with the previous conclusion observed from other food proteins. 17,27 BS might be incorporated into the compact cross-β sheet structure in amyloid aggregates, forming small hydrophobic microdomains above their cmc and thus disrupting the protein aggregates. It has been demonstrated that the hydrophobic pockets in the protein−BS complexes are less polar than the core of the BS micelles.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…In addition, the aggregation and assembly of bile components produce physiologically relevant colloidal structures, including mixed micelles, vesicles, and lipid droplets with different sizes and morphologies . A recent study has showed that the digestion of β-lactoglobulin with human bile could be closely replicated by BS/phosphatidylcholine mixtures, which presented an example of human-relevant bile for in vitro proteolysis studies . However, how these colloidal structures interact with food proteins and their implications in protein digestion regarding the potential allergenicity are still unknown.…”

Section: Introductionmentioning

confidence: 99%

“…26 A recent study has showed that the digestion of β-lactoglobulin with human bile could be closely replicated by BS/phosphatidylcholine mixtures, which presented an example of human-relevant bile for in vitro proteolysis studies. 27 However, how these colloidal structures interact with food proteins and their implications in protein digestion regarding the potential allergenicity are still unknown.…”

Section: ■ Introductionmentioning

confidence: 99%

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Bile Acid Profile Influences Digestion Resistance and Antigenicity of Soybean 7S Protein

Han

Feng

et al. 2023

J. Agric. Food Chem.

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Soybean 7S storage protein (β-conglycinin) is the most important allergen, exhibits resistance in gastrointestinal (GI) digestion, and causes allergies in humans and animals. A previous study has demonstrated that 7S proteins contained innate amyloid aggregates, but the fate of these specific protein aggregates in intestinal digestion and correlation to allergenicity are unclear. In this study, via a modified INFOGEST static in vitro digestion and IgE binding test, we illustrate that the survived amyloid aggregates of soybean 7S protein in GI digestion might be dominant IgE epitopes of soybean protein in humans. The impact of conjugated primary bile acid salt (BS) profile on digestion resistance and immunogenicity of soybean protein is assessed, regarding the binding affinity of BS to protein aggregates with consideration of the BS composition and the physiologically relevant colloidal structure. The results show that chenodeoxycholate-containing colloidal structures exhibit high affinity and unfolding capacity to protein amyloid aggregates, promoting proteolysis by pancreatic enzymes and thus mitigating the antigenicity of soybean protein. This study presents a novel understanding of bile acid profile and colloidal structure influence on the digestibility and antigenicity of dietary proteins. It should be helpful to design in vitro digestion protocol and accurately replicate physiologically relevant digestion conditions.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Bile Acid Profile Influences Digestion Resistance and Antigenicity of Soybean 7S Protein

Han

Feng

et al. 2023

J. Agric. Food Chem.

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“…The EHC is schematically represented in Figure . The total pool of bile acids in the gastrointestinal tract is ∼3 g, which recirculates ∼4 to 12 times per day or as many as 5 or more times for a single fat-rich meal. − If reabsorption of bile acids is defective, as can occur after resection of the ileum (bariatric surgery), de novo synthesis of bile acids by the liver can be as high as 4 to 6 g/day. − The bile that is stored in the gall bladder is mostly composed of water (95%), conjugated bile salts, bile pigments (bilirubin and biliverdin), fats (cholesterol, lecithin), and inorganic salts. − …”

Section: Bile Acids: Synthesis and Enterohepatic Circulation/bile Poolmentioning

confidence: 99%

Bile Acid Sequestrants for Hypercholesterolemia Treatment Using Sustainable Biopolymers: Recent Advances and Future Perspectives

et al. 2022

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Bile acids, the endogenous steroid nucleus containing signaling molecules, are responsible for the regulation of multiple metabolic processes, including lipoprotein and glucose metabolism to maintain homeostasis. Within our body, they are directly produced from their immediate precursors, cholesterol C (low-density lipoprotein C, LDL-C), through the enzymatic catabolic process mediated by 7-α-hydroxylase (CYP7A1). Bile acid sequestrants (BASs) or amphiphilic resins that are nonabsorbable to the human body (being complex high molecular weight polymers/electrolytes) are one of the classes of drugs used to treat hypercholesterolemia (a high plasma cholesterol level) or dyslipidemia (lipid abnormalities in the body); thus, they have been used clinically for more than 50 years with strong safety profiles as demonstrated by the Lipid Research Council–Cardiovascular Primary Prevention Trial (LRC–CPPT). They reduce plasma LDL-C and can slightly increase high-density lipoprotein C (HDL-C) levels, whereas many of the recent clinical studies have demonstrated that they can reduce glucose levels in patients with type 2 diabetes mellitus (T2DM). However, due to higher daily dosage requirements, lower efficacy in LDL-C reduction, and concomitant drug malabsorption, research to develop an “ideal” BAS from sustainable or natural sources with better LDL-C lowering efficacy and glucose regulations and lower side effects is being pursued. This Review discusses some recent developments and their corresponding efficacies as bile removal or LDL-C reduction of natural biopolymer (polysaccharide)-based compounds.

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Nanomedicine based potentially transformative strategies for colon targeting of peptides: State-of-the-art

Vambhurkar

Amulya

Sikder

et al. 2022

Colloids and Surfaces B: Biointerfaces

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The bile salt content of human bile impacts on simulated intestinal proteolysis of β-lactoglobulin

Cited by 11 publications

References 45 publications

Bile Acid Profile Influences Digestion Resistance and Antigenicity of Soybean 7S Protein

Bile Acid Profile Influences Digestion Resistance and Antigenicity of Soybean 7S Protein

Bile Acid Sequestrants for Hypercholesterolemia Treatment Using Sustainable Biopolymers: Recent Advances and Future Perspectives

Nanomedicine based potentially transformative strategies for colon targeting of peptides: State-of-the-art

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