The binding and utilization of hemoglobin by Prevotella intermedia

Leung, Kai P.

doi:10.1016/s0378-1097(98)00126-8

Cited by 21 publications

(28 citation statements)

References 15 publications

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“…The observation that globin strongly inhibited hemoglobin‐binding activity suggests that the globin moiety of the hemoglobin molecule may be essential for binding to P. nigrescens . Similar results were also documented for P. gingivalis (15) and P. intermedia (17). Duchesne et al.…”

Section: Discussionsupporting

confidence: 86%

Characterization of binding and utilization of hemoglobin by Prevotella nigrescens

Guan

Nagata

Kuboniwa

et al. 2002

Oral Microbiology and Immunology

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The ability of Prevotella nigrescens to utilize and bind to hemoglobin was investigated. Growth studies showed that P. nigrescens was able to utilize hemoglobin efficiently as an iron source. Binding of P. nigrescens to hemoglobin was demonstrated by dot blot assay. Heat and trypsin treatments of the bacteria led to a decrease in activity. Globin gave nearly complete inhibition of activity. Additionally, lactoferrin partially inhibited activity. In contrast, transferrin, cytochrome C and catalase exerted little or no inhibitory effect. Although the sugars tested did not affect activity, several of the amino acids tested, including arginine, cysteine, histidine and lysine, inhibited activity. In a solid phase assay, 41-, 56- and 59-kDa proteins of P. nigrescens reacted with hemoglobin. These results suggest that P. nigrescens utilizes hemoglobin for growth and 41-, 56- and 59-kDa proteins may be involved in hemoglobin binding.

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Section: Discussionsupporting

confidence: 86%

Characterization of binding and utilization of hemoglobin by Prevotella nigrescens

Guan

Nagata

Kuboniwa

et al. 2002

Oral Microbiology and Immunology

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“…The phenomenon has been noted in a group of anaerobic species that are mainly oral pathogens and were previously known as black-pigmented Bacteroides species but have now been reclassified as Porphyromonas and Prevotella species (17). These bacteria depend largely on external heme (either hemoglobin or hemopexin) to satisfy their demand for iron (20) and to a greater or lesser extent accumulate a black pigment intracellularly that consists of an iron-containing heme aggregate (e.g., hematin) together with various amounts of iron-free PPIX (28,29). If they accumulate PPIX they will be photosensitive (13), however, if they mainly have hematin they will not be photo- showed that these species were more sensitive when grown on media containing hemin as an iron source compared to hemoglobin (14).…”

Section: Discussionmentioning

confidence: 99%

Helicobacter pylori Accumulates Photoactive Porphyrins and Is Killed by Visible Light

Hamblin

Viveiros

Yang

et al. 2005

Antimicrob Agents Chemother

207

161

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Helicobacter pylori colonizes the mucus layer of the human stomach and duodenum, causes chronic gastritis, gastric ulcer, and is a risk factor for gastric adenocarcinoma. There is a 20% failure rate in antibiotic therapy, which is increasingly due to antibiotic resistance and necessitates the search for alternative antimicrobial methods. We have discovered that H. pylori when cultured in liquid medium, accumulates significant quantities of coproporphyrin and protoporphyrin IX, both in the cells and secreted into the medium. These photoactive porphyrins lead to cell death (up to 5 logs) by photodynamic action upon illumination with low doses of visible light, with blue/violet light being most efficient. The degree of killing increases with the age of the culture and is greater than that found with Propionibacterium acnes (another bacterium known to be photosensitive due to porphyrin accumulation). Both virulent and drug-resistant strains are killed. The data suggest that phototherapy might be used to treat H. pylori infection in the human stomach.Helicobacter pylori is a gram-negative microaerophilic bacterium which selectively colonizes the mucus layer of the human stomach and duodenum (7). As more than 50% of the world population is infected and in some countries infection rates approach 90%, H. pylori can be termed the world's commonest infectious agent (19). H. pylori infection has been shown to be strongly associated with the presence of inflammation and chronic gastritis, and once acquired, H. pylori persists, usually for life, unless eradicated by antimicrobial therapy (15). It is now known that H. pylori is a major cause of peptic ulcer disease, and in 1994, the International Agency for Cancer Research declared that H. pylori was a carcinogen of humans and was implicated in the development of gastric cancer (11).The most common antibacterial treatment regimens include bismuth, metronidazole, tetracycline, and a proton pump inhibitor, or clarithromycin in combination with a proton pump inhibitor and amoxicillin (10). Combinations such as the above lead to eradication rates of about 80% but at the expense of side effects and possible poor patient compliance. Increasing development of antibiotic resistance among H. pylori isolates and the existence of nonresponsive patients suggest that alternative strategies for H. pylori eradication be sought (22).Photodynamic therapy uses the combination of nontoxic dyes (frequently porphyrins or their derivatives) and harmless visible light to produce cytotoxicity via generation of reactive oxygen species (25). Photodynamic therapy has been clinically approved for various malignant, premalignant, and ophthalmologic conditions (6). It has long been known that many microorganisms including gram-negative and gram-positive bacteria, mycoplasma, fungi, and viruses can be killed by photodynamic therapy, and recently photodynamic therapy has been investigated as a treatment for infectious disease (12). The grampositive bacterium that causes acne, Propionibacterium acnes, is kil...

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“…Oral black‐pigmented anaerobes, such as Porphyromonas gingivalis and Prevotella intermedia, are putative periodontal pathogens (14, 18, 24). These bacteria have been frequently isolated from subgingival plaque in patients suffering from adult periodontitis (25).…”

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confidence: 99%

Distribution of the tetracycline resistance determinant tetQ gene in oral isolates of black‐pigmented anaerobes in Japan

Okamoto

Takano

Maeda³

2001

Oral Microbiology and Immunology

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We investigated the distribution of tetracycline resistance determinant tetQ in oral black-pigmented anaerobes using a polymerase chain reaction (PCR) METHOD: A total of 185 healthy subjects were divided into 3 groups based on subject age: young (6 to 10 years, n=58), middle (11 to 40 years, n=96), and elder (exactly 70 years, n=31). The prevalence of black-pigmented anaerobes in the gingival sulcus among these groups was 29.3%, 28.2%, and 64.5%, respectively. The prevalence of Prevotella nigrescens among these groups was 22.4%, 15.6%, and 32.3%, respectively, whereas the prevalence of Prevotella intermedia was 1.7%, 4.2%, and 35.5%, respectively. Porphyromonas gingivalis was found only in the elder group (16.1%). The prevalence of the tetQ gene in the black-pigmented anaerobes-positive subjects was almost the same among the 3 groups (approximately 30%). The tetQ gene was found in 27.5% (46 of 167) of P. nigrescens isolates, whereas it was found in only 6.4% (3 of 47) of P. intermedia isolates and in none of the 19 P. gingivalis isolates. Restriction endonuclease digestion patterns of the PCR products revealed 83.6% of 49 tetQ-positive isolates were of subtype A2H2 (AluI type 2, HpaII type 2).

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The binding and utilization of hemoglobin by Prevotella intermedia

Cited by 21 publications

References 15 publications

Characterization of binding and utilization of hemoglobin by Prevotella nigrescens

Characterization of binding and utilization of hemoglobin by Prevotella nigrescens

Helicobacter pylori Accumulates Photoactive Porphyrins and Is Killed by Visible Light

Distribution of the tetracycline resistance determinant tetQ gene in oral isolates of black‐pigmented anaerobes in Japan

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