1982
DOI: 10.1111/j.1471-4159.1982.tb10868.x
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The Binding of [3H]AMPA, a Structural Analogue of Glutamic Acid, to Rat Brain Membranes

Abstract: Binding of [3H]AMPA to rat brain membranes was investigated. The binding was saturable and reversible at physiological pH. Computer-aided Scatchard analysis of the binding data, as determined by using L-glutamic acid (L-GLU) to define nonspecific binding, suggested the presence of two independent binding sites, with KDS of 9 and 2440 nM, respectively. Additional freezing, thawing and washing sequences gave membranes with only one binding site, with a KD of 278 nM. [3H]AMPA binding exhibited the highest level i… Show more

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Cited by 232 publications
(85 citation statements)
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“…Substances blocking transmission at the giant synapse included not only L-glutamate and kainate (cf. De Santis, Eusebi & Miledi, 1978) but also quisqualate and the selective agonist, AMPA (Krogsgaard-Larsen, Honore, Hansen, Curtis & Lodge, 1980;Honore, Lauridsen & Krogsgaard-Larsen, 1982). We assume that all these four substances are acting by desensitizing the postsynaptic membrane.…”
Section: Res Ultsmentioning
confidence: 99%
“…Substances blocking transmission at the giant synapse included not only L-glutamate and kainate (cf. De Santis, Eusebi & Miledi, 1978) but also quisqualate and the selective agonist, AMPA (Krogsgaard-Larsen, Honore, Hansen, Curtis & Lodge, 1980;Honore, Lauridsen & Krogsgaard-Larsen, 1982). We assume that all these four substances are acting by desensitizing the postsynaptic membrane.…”
Section: Res Ultsmentioning
confidence: 99%
“…The glutamate receptors essential for fast excitation are classified into N-methyl-D-aspartate (NMDA)-sensitive and -insensitive receptors, and in the latter, separate types recognizing L-kainic acid (KA) and AMPA (1) have been generally accepted (2). However, electrophysiological evidence is accruing for unitary non-NMDA receptors of KA and AMPA selectivity in situ (for review, see ref.…”
mentioning
confidence: 99%
“…!ed 6-nitro-7-sulphamoy!benzo [f ] quinoxa!ine-2,3-dione ([3H I -NBQX) (Dev et a!., 1996). The two agonists, [3H]- fluorowi!lardiine, bound with large affinity differences to two sites in rat brain membranes (Honoré et a!., 1982;Morgan et al, 1991;Hawkins et a!., 1995;Hawkinson and Espitia, 1997), and the molecular identity of these two sites is a matter of debate, particularly in relation to the binding properties of the cloned homomeric G!uRl-4 (Ho! !-man and Heinemann, 1994).…”
mentioning
confidence: 99%
“…Several radioligands have been used to characterize AMPA receptors and their ligands in rat brain: [3H]-AMPA (Honoré et a!., 1982), 3H-!abel!ed 6-cyano-7-nitroquinoxaline-2,3-dione ([3H] CNQX) (Honoré et a!., 1989), 5-[3H]fluorowillardiine (Hawkins et a!., 1995), 3H-!abelled 1 ,2,3,6,7,8-hexahydro-3-(hydroxyimino )-N,N, 7-trimethy!-2-oxobenzo [2,1 -b:3,4-c'] dipyrro!e-5-sulphonamide ([3H] NS 257) (Nielsen et al, 1995), and 3H-!abe! !ed 6-nitro-7-sulphamoy!benzo [f ] quinoxa!ine-2,3-dione ([3H I -NBQX) (Dev et a!., 1996).…”
mentioning
confidence: 99%