The Biocatalysis in Cancer Therapy

Liu, Junling; Ren, Huiping; Tang, Tong; Wang, Jiaqi; Fang, Jiali; Huang, Chenming; Zheng, Zhendong; Qin, Bin

doi:10.1021/acscatal.3c01363

Cited by 6 publications

(3 citation statements)

References 249 publications

(484 reference statements)

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“…Biocatalysis has made an impact in the synthesis of chemicals and pharmaceuticals over the last years. Beyond catalysis in the world of low-molecular-weight compounds, there is also the opportunity to use enzymes as therapeutics with various modes of action. Many therapeutic enzymes have been already approved by the FDA and brought to market .…”

Section: Applications In Biocatalysis and The Pharmaceutical Industrymentioning

confidence: 99%

Accelerating Biocatalysis Discovery with Machine Learning: A Paradigm Shift in Enzyme Engineering, Discovery, and Design

Markus,

Andreas

et al. 2023

ACS Catal.

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Emerging computational tools promise to revolutionize protein engineering for biocatalytic applications and accelerate the development timelines previously needed to optimize an enzyme to its more efficient variant. For over a decade, the benefits of predictive algorithms have helped scientists and engineers navigate the complexity of functional protein sequence space. More recently, spurred by dramatic advances in underlying computational tools, the promise of faster, cheaper, and more accurate enzyme identification, characterization, and engineering has catapulted terms such as artificial intelligence and machine learning to the must-have vocabulary in the field. This Perspective aims to showcase the current status of applications in pharmaceutical industry and also to discuss and celebrate the innovative approaches in protein science by highlighting their potential in selected recent developments and offering thoughts on future opportunities for biocatalysis. It also critically assesses the technology’s limitations, unanswered questions, and unmet challenges.

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Section: Applications In Biocatalysis and The Pharmaceutical Industrymentioning

confidence: 99%

Accelerating Biocatalysis Discovery with Machine Learning: A Paradigm Shift in Enzyme Engineering, Discovery, and Design

Markus,

Andreas

et al. 2023

ACS Catal.

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“…The biological importance of enzymes has generated considerable interest in their study, with a significant focus on the elucidation of their structure and catalytic properties. Detailed knowledge of biologically significant enzymes has perhaps proven most influential in the pharmaceutical and medical fields, where advances in their chemistry has facilitated a better understanding of the mechanism of diseases and the development of more effective treatments [ 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

Enzymes in “Green” Synthetic Chemistry: Laccase and Lipase

Scheibel,

Gitsov,

Gitsov

2024

Molecules

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Enzymes play an important role in numerous natural processes and are increasingly being utilized as environmentally friendly substitutes and alternatives to many common catalysts. Their essential advantages are high catalytic efficiency, substrate specificity, minimal formation of byproducts, and low energy demand. All of these benefits make enzymes highly desirable targets of academic research and industrial development. This review has the modest aim of briefly overviewing the classification, mechanism of action, basic kinetics and reaction condition effects that are common across all six enzyme classes. Special attention is devoted to immobilization strategies as the main tools to improve the resistance to environmental stress factors (temperature, pH and solvents) and prolong the catalytic lifecycle of these biocatalysts. The advantages and drawbacks of methods such as macromolecular crosslinking, solid scaffold carriers, entrapment, and surface modification (covalent and physical) are discussed and illustrated using numerous examples. Among the hundreds and possibly thousands of known and recently discovered enzymes, hydrolases and oxidoreductases are distinguished by their relative availability, stability, and wide use in synthetic applications, which include pharmaceutics, food and beverage treatments, environmental clean-up, and polymerizations. Two representatives of those groups—laccase (an oxidoreductase) and lipase (a hydrolase)—are discussed at length, including their structure, catalytic mechanism, and diverse usage. Objective representation of the current status and emerging trends are provided in the main conclusions.

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“…[2] This strategy has facilitated a broad spectrum of enzymatic modifications, including enhancements in catalytic activity, substrate specificity, and thermal stability, as well as the creation of completely novel enzymatic functions. [3] Consequently, biocatalysts are being increasingly harnessed as useful tools for the green synthesis of fine chemicals and pharmaceuticals, [4] for modifying biological therapeutics, [5] and even for degrading plastic waste, [6] thereby contributing to more sustainable industrial processes. [7] Oxazolidinones, one of the valuable heterocyclic compounds that contain both oxygen and nitrogen atoms in a five-membered ring, have attracted remarkable attention in organic synthesis and medicinal chemistry.…”

mentioning

confidence: 99%

Biocatalytic Regio‐ and Enantiocomplementary Synthesis of Chiral Aryloxazolidinones

Zhou,

Wang,

et al. 2024

Adv Synth Catal

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We described the protein engineering of the halohydrin dehalogenase HheG to enhance its R enantioselectivity for the synthesis of chiral 4‐aryloxazolidinones via α‐regioselective ring‐opening of aryl epoxides with cyanate. Additionally, we achieved the inversion of its regioselectivity and enantioselectivity, resulting in a HheG variant tailored for the synthesis of chiral 5‐aryloxazolidinones via β‐regioselective and S‐enantioselective ring‐opening of aryl epoxides with cyanate. Leveraging these engineered mutants, we developed a biocatalytic platform capable of synthesis of both chiral 4‐aryloxazolidinones (up to 47% yield, 98% ee, and 99% regioselectivity) and chiral 5‐aryloxazolidinones (up to 46% yield, >99% ee, and 98% regioselectivity). Furthermore, a collaborative approach utilizing two regio‐ and enantioselective HheG mutants has been demonstrated to enable the simultaneous synthesis of chiral 4‐aryloxazolidinones and chiral 5‐aryloxazolidinones.

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The Biocatalysis in Cancer Therapy

Cited by 6 publications

References 249 publications

Accelerating Biocatalysis Discovery with Machine Learning: A Paradigm Shift in Enzyme Engineering, Discovery, and Design

Accelerating Biocatalysis Discovery with Machine Learning: A Paradigm Shift in Enzyme Engineering, Discovery, and Design

Enzymes in “Green” Synthetic Chemistry: Laccase and Lipase

Biocatalytic Regio‐ and Enantiocomplementary Synthesis of Chiral Aryloxazolidinones

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