The Biochemistry of the Nucleic Acids

Adams, Roger; Knowler, John T.; Leader, David P.

doi:10.1007/978-94-011-2290-0

Cited by 82 publications

(52 citation statements)

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“…Bands a, b and f fluoresced bright red and were identified as 58S rRNA. 5S rRKA and 4S tRNA respectively, by comparison of their relative mobilities with published data (Loenig, 1967;Callow et al, 1978;Adams et al, 1981). The pigment bands autofluoresced orange.…”

Section: Resultsmentioning

confidence: 79%

“…Polyphosphate should stain pink, RNA blue-purple (Callow et al, 1978) and phenolic compounds bright blue or green (O'Brien & McCully, 1981). PolypViosphate was further differentiated from nucleic acid bands by staining with ethidium bromide (1 mg ml"') for 15 n:iin and examination ut\der long wave UV light (Adams et al, 1981;Inouye & Dudock, 1987). Gels were photographed on a light box on Kodak Technical Pan (green filter) or Ektachrome film, or on Polaroid film under UV light.…”

Section: Pagementioning

confidence: 99%

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Demonstration of a short chain polyphosphate in Pisolithus tinctorius and the implications for phosphorus transport

1994

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SUMMARYPolyphosphate was extracted from an Australian isolate oi Pisolithus tinctorius (Pers.) Coker & Couch, known to he mycorrhizal with Eucalyptus pilularis. It was separated from RNA and characterized hy PAGE and ^^F NMR spectroscopy. A broad pink hand, which showed y-metachromasy with toiuidine blue, ran faster on acrylamide gels than a number of purple-blue bands (various RNA fractions) and did not stain with ethidium bromide, was identified as polyphosphate. The major pink band co-migraied with a synthetic polyphosphate standard of a chain length of about 1 5 phosphate (PJ subunits, and analysis by ^^P nuclear magnetic resonance (NMR) spectroscopy confirmed that polyphosphate was a major fraction in the extract. Polyphosphate was extracted from mycelium grown at both high and low levels of P,, but the amount extracted from cultures grown with 10 mM P, (added as ammonium phosphate) was significantly greater, Polyphosphate was a substantial fraction of the phosphorus present in the hyphae and it is proposed that polyphosphate is transported along the hyphae by the motile tubule and vacuole system present in this fungus.

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Section: Resultsmentioning

confidence: 79%

Section: Pagementioning

confidence: 99%

Demonstration of a short chain polyphosphate in Pisolithus tinctorius and the implications for phosphorus transport

1994

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“…Similar sequences are also evident at the equivalent positions upstream from the AUG of the 25 K genes of LSV and PVM, at approximately 40 nucleotides from their respective initiation eodons. These blocks are most striking when compared with the well defined ribosome binding sites of E, coil RNA polymerase# and the A protein of the E. colt phage MS2 as shown in Figs 2B and 2C [16].…”

Section: Resultsmentioning

confidence: 90%

Occurrence of chloroplast ribosome recognition sites within conserved elements of the RNA genomes of carlaviruses

Foster

Mills

1991

FEBS Letters

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The nucl¢otide sequences upstream from the ,=~rlavirus olin reading frames wcrc exan~ined for direct sequence homology, Blocks of homology were evident upstream from the 25 K ORFs eF potato virus S (PVS), potato virus M (PVM} and lily symptomless virus (LSV), and upstream from th,= coast protein initiation codons of PVS, PVM, LSV. carnation latent virus and Hdenium virus S, Then,= blocks, which correspond to the Y.terminal resions of the subjenomic RNAs. were sl~own to contain potential ribosome rcco~,nitlon sequences, The distances between tit,= bindtn8 sites and initiation codons ransed from 20 to 40 nucleotides on the viral RNAs, Whilsl tit,= m.'tjority of chloroplasts mRNAs have a distance of 8 nucleotides between bindin8 site and initiation codon, the remaininB have a distance o1" 23 nucleotides which is similar to that reported here for the carlaviru~s.Subgenomic promoter: Seq nonce homology; Carlavirus; Ribosome bindin6 sit'=

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“…Samples were frozen at −70°C and then freeze-dried by pumping under the pressure of 1-3 mTorr for two hours. The film thickness has been estimated as previously described (Alizadeh et al 2011;Goodhead, 1990) by taking 6.0 ± 0.2 mm as the diameter of the DNA film, a thickness of 2 nm for each ML of plasmid and the known density of 1.71 g/cm 3 of the plasmid extracted from E. coli (Adams et al, 1986). This procedure resulted in a dry film of 10 nm average thickness on the substrates, assuming minimal clustering of the plasmids.…”

Section: Plasmid Dna Preparationmentioning

confidence: 99%

Induction of strand breaks in DNA films by low energy electrons and soft X-ray under nitrous oxide atmosphere

Alizadeh

Sanche

2012

Radiation Physics and Chemistry

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Five-monolayer (5ML) plasmid DNA films deposited on glass and tantalum substrates were exposed to Al K α X-rays of 1.5 keV under gaseous nitrous oxide (N 2 O) at atmospheric pressure and temperature. Whereas the damage yields for DNA deposited on glass are due to soft X-rays, those arising from DNA on tantalum are due to both the interaction of low energy photoelectrons from the metal and X-rays. Then, the differences in the yields of damage on glass and tantalum substrates, essentially arises from interaction of essentially low-energy electrons (LEEs) with DNA molecules and the surrounding atmosphere. The G-values (i.e., the number of moles of product per Joule of energy absorbed) for DNA strand breaks induced by LEEs (G LEE ) and the lower limit of G-values for soft X-ray photons (G XL ) were calculated and the results compared to those from previous studies under atmospheric conditions and other ambient gases, such as N 2 and O 2 . Under N 2 O, the G-values for loss of supercoiled DNA are 103±15 nmol/J for X-rays, and 737±110 nmol/J for LEEs. Compared to corresponding values in an O 2 atmosphere, the effectiveness of X-rays to damage DNA in N 2 O is less, but the G value for LEEs in N 2 O is more than twice the corresponding value for an oxygenated environment. This result indicates a higher effectiveness for LEEs relative to N 2 and O 2 environments in causing SSB and DSB in an N 2 O environment. Thus, the previously observed radiosensitization of cells by N 2 O may not be only due to OH • radicals but also to the reaction of LEE with N 2 O molecules near DNA. The previous experiments with N 2 and O 2 and the present one demonstrate the possibility to investigate damage induced by LEEs to biomolecules under various type of surrounding atmospheres.

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The Biochemistry of the Nucleic Acids

Cited by 82 publications

References 0 publications

Demonstration of a short chain polyphosphate in Pisolithus tinctorius and the implications for phosphorus transport

Demonstration of a short chain polyphosphate in Pisolithus tinctorius and the implications for phosphorus transport

Occurrence of chloroplast ribosome recognition sites within conserved elements of the RNA genomes of carlaviruses

Induction of strand breaks in DNA films by low energy electrons and soft X-ray under nitrous oxide atmosphere

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