The biocompatibility of resin composite materials on different stem cells.

Abstract

Objectives: Composite resins should undergo attentive tests to specify their biocompatibility before they have contact with the teeth, regardless of physico-mechanical properties. Hence, this study has been carried out to assess the knowledge of potential cellular responses and biological effects of composite resins on three different cells after two different exposure times. Methods: The composites Surefil SDR flow, Dentsply Caulk/ABD (SS), X-tra base flowable, Voco Cuxhaven Germany (XB), Venus Bulk Flow, Voco Cuxhaven Germany (VB), Filtek Bulk Flow, 3 M/ ESPE-USA (FB), Tetric-Evo bulk flow, Ivoclar Vivadent, Schaan, Liechtenstein (TE) were immersed into molds (Height: 4 mm, Width: 6 mm) and polymerized according to manufacturer's instructions. Human gingival fibroblasts (HGFs), cortical neuron cells (CNCs) and dental pulp stem cells (DPSCs) were inoculated into 24-well plates, separately. Then, the insert membranes with a 3 µm pore width compatible for plates were inserted and dental composite samples were added. The experimental procedures were performed after 24 h and 72 h. The cell viability of the cells was obtained from an MTT assay. Total antioxidant (TAS) and oxidant (TOS) status were examined by using real assay diagnostic kits. The dynamic process of apoptosis analysed by using annexin V-FITC staining with flow cytometry. Human beta defensins were assessed by RT-PCR. Lastly, supernatants were analysed for production of IL6, IL8 and IL10 cytokines. Results: According to analysis of variance, there were significant differences between three cells. There was an increase in the cytotoxicity values in some groups after 72 h. A 24 h and 72 h experiment revealed that VB was the strongest cytotoxic agent, followed sequentially by XB and FB. Exposure of cells to the composites of SS, XB, VB, FB and TE for 24 h reduced the cell number approximately by 6, 24, 35.6, 28.6 and 1%. A 72 h reduced the cell number for 25.3, 31.6, 40.6, 50.3 and 8%, respectively, by SS, XB, VB, FB and TE. Conclusions: It can be concluded that from all these data, if the three cell cultures are compared, cortical neuron cells were more sensitive to the resin composites.

The Effect of Flowable Composite Resins on Periodontal Health, Cytokine Levels, and Immunoglobulins

“…An increase in IL-1 levels also triggers the release of the anti-inflammatory cytokine IL-10 to restrain the increase of inflammation and tissue destruction [ 27 ]. Kamalak et al showed that after 24 hours of applying two different FCRs, the IL-6 and IL-8 levels secreted from the cells were balanced by increased IL-10 secretion [ 28 ]. Similarly, in this study, a significant IL-10 storm was detected in all groups at the end of the first day and increased up to 3-4 times from the baseline.…”

Effect of two different materials in sealing vertical root fractures of intentional replantation on epithelial thickness of periradicular tissue

“…9 Furthermore, the LED light cure unit, which was used in this study to polymerize the resin cement, might also cause a negative effect because this light source may not completely polymerize the resin cement. 8 Subsequently, free monomer components, such as Bis-GMA, UDMA, TEGDMA camphorquinone, and HEMA, may be found. These free monomers have the potential to cause pulp injury and prevent a proper tissue healing process.…”

“…Thus, self-adhesive resin cement is proposed to be utilized to seal vertical root fractures that will simplify the treatment. 8,9 Thus far, mineral trioxide aggregate (MTA) is a material of choice to seal the fracture gap since it has many beneficial properties, such as biocompatibility, closure density, mechanical strength, and the healing capacity of periradicular tissue. 10,11 Even though the clinical success of employing composite resin in intentional replantation showed more than 90% after two years following the treatment, 7 it is still necessary to evaluate its success histologically to evaluate the regeneration of periradicular tissue, particularly the epithelial thickness.…”