2018
DOI: 10.1101/483644
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The biogenesis of CLEL peptides involves several processing events in consecutive compartments of the secretory pathway

Abstract: AbstractPost-translationally modified peptides are involved in many aspects of plant growth and development. The maturation of these peptides from their larger precursors is still poorly understood. We show here that the biogenesis of CLEL6 and CLEL9 peptides in Arabidopsis thaliana requires a series of processing events in consecutive compartments of the secretory pathway. Following cleavage of the signal peptide upon entry into the endoplasmic retic… Show more

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Cited by 3 publications
(5 citation statements)
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“…Before GLVs are secreted and exert their physiological role, they require maturation through consecutive cleavage events by SBTs on the peptide precursors' passage through the secretory pathway. Pre-processing of GLV2 is executed by SBT6.1/S1P (Ghorbani et al, 2016;Stührwohldt et al, 2020). Intriguingly, S1P is additionally maturating RALF23 peptides which attenuate immunity by inhibiting the PRR scaffolding function of FER (Srivastava et al, 2008;Stegmann et al, 2017).…”
Section: Discussionmentioning
confidence: 99%
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“…Before GLVs are secreted and exert their physiological role, they require maturation through consecutive cleavage events by SBTs on the peptide precursors' passage through the secretory pathway. Pre-processing of GLV2 is executed by SBT6.1/S1P (Ghorbani et al, 2016;Stührwohldt et al, 2020). Intriguingly, S1P is additionally maturating RALF23 peptides which attenuate immunity by inhibiting the PRR scaffolding function of FER (Srivastava et al, 2008;Stegmann et al, 2017).…”
Section: Discussionmentioning
confidence: 99%
“…Similar results were obtained in at least 3 independent experiments. GLV2 requires sulfation at a conserved tyrosine residue by TPST to control meristematic activity and root gravitropism (Matsuzaki et al, 2010;Stührwohldt et al, 2020). The tpst mutants show increased immune responses which was mainly linked to PSK and PSY1-mediated immune regulation, both of which are also substrates of TPST (Igarashi et al, 2012;Mosher et al, 2013).…”
Section: Glv2 Peptides Increase Sensitivity Of Plants To Flg22 Percepmentioning
confidence: 99%
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“…Primers used to amplify sequences for the creation of new GreenGate entry vectors are given in Supplemental Table S3. GFP, mTurquoise and mCherry modules (pGGD011, pGGD-GSL-mTurquoise, pGGD010 and pGGF Alli-mCherry) were published before (Lupanga et al, 2020;Stührwohldt et al, 2020;Waadt et al, 2017;Stephani et al, 2020). Internal BsaI sites were mutated.…”
Section: Cloningmentioning
confidence: 99%
“…The signal peptide is cleaved in the ER to release the mature propeptide containing the variable domain and the C-terminal CLE peptide. The propeptide undergoes post-translational modification during its transit through the ER and Golgi apparatus, resulting, in particular, in a CLE motif decorated with hydroxyproline and arabinosyl residues (Matsubayashi, 2011;St€ uhrwohldt et al, 2020) (Fig. 1a).…”
mentioning
confidence: 99%