The Biologic Activities of Cystic Fibrosis Serum. II. Ultrastructural Aspects of the Effect of Cystic Fibrosis Sera and Calcium Ionophore A23187 on Rabbit Tracheal Explants

Bogart, Bruce Ian; Conod, Elaine J; Gaerlan, Pureza F.; Conover, James H.

doi:10.1203/00006450-197801000-00005

Cited by 14 publications

(4 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The role of calcium in the regulation of ciliary activity in mammalians is being elucidated (14,18). The present data, as well as our recent observations (18), are compatible with but do not prove the interpretation put forth by Conover et al (8) and Bogart et al (3) to explain the mechanism of action of CF factor(s).…”

Section: T E M P E R a T U R Esupporting

confidence: 81%

Laser Light-Scattering Spectroscopy: Preliminary Results on Bioassay of Cystic Fibrosis Factor(s)

Verdugo

Vincenzi

1979

Pediatr Res

View full text Add to dashboard Cite

Summaryspeed cinematography has shown that the laser technique renders precise measurement of the frequency of ciliary beating (13). The The effect of cystic fibrosis sera and sera was present paper reports on preliminary results obtained by laser investigated in 37 cultures of ciliated epithelium of the rabbit light-rattering spectroscopy in detecting and recording the effect trachea. Serum was introduced in the Rose culture chambers in a of CF and normal sera on the ciliary activity of cultures of ciliated concentration of 10% by volume. The frequency of ciliary beat was cells of rabbit trachea. monitored by laser light-scattering spectroscopy. The results show that the response of cilia to sera canbe well characterized by laser light scattering. Our preliminary evidence suggests that an early MATERIALS AND METHODSincrease in the frequency of ciliary beat, followed by rapid arrest of ciliary activity, is a sensitive and characteristic response pro-SERUM SAMPLES duced by cystic fibrosis serum, and not by normal serum.Blood was collected from six C F patients attending the Cystic Fibrosis Clinic at the Children's Orthopedic Hospital in Seattle, SpeculationWashington. The criterion for inclusion was elevated sweat chloThe present work was aimed at demonstrating the feasibility of ride. None of the patients had asthma or other respiratory diseases. implementing an objective and quantifiable bioassay for cystic subjects were e m~l o~e d ' was drawn into fibrosis factor@). Laser light-scattering spectroscopy is applicable plain evacuated drawing tubes. The were to detection and quantification of ciliary activity. The method may 'lot and then stored in plastic tubes at 40 for hr' lead to improved bioassays for cystic fibrosis factor(s).Coagulated blood samples were centrifuged in the cold and sera were then frozen and assayed within 7-10 days after collection. Several assays were also performed on a pool of samples of C F The observation that serum as well as other biologic fluids from serum that had been frozen and stored in plastic tubes for patients with cystic fibrosis (CF) can interfere with ciliary activity months. has attracted a great deal of interest. Ciliated cells are responsive to so-called C F factor(s) present in serum (2,5,7,17), urine (4), TISSUE CULTURES and saliva (9). The establishment of an objective and quantifiable bioassay to detect the presence of ciliotoxic factor(s) would be a New Zealand White adult rabbits were stunned and their useful step toward substantiating the diagnosis of CF on a reliable tracheae rapidly removed. To ensure sterility, the whole trachea was camed through four dishes of sterile Hanks' culture medium biochemical or immunochemical basis. A common assumption (pH 7.3) at room temperature. In the first dish extraneous tissue has been that a quantifiable bioassay for cystic fibrosis, based on was trimmed off and the trachea was slit longitudinally. Several the effect of CF serum on ciliary activity, has already been developed. However, visual observation of cilia cannot ...

show abstract

Section: T E M P E R a T U R Esupporting

confidence: 81%

Laser Light-Scattering Spectroscopy: Preliminary Results on Bioassay of Cystic Fibrosis Factor(s)

Verdugo

Vincenzi

1979

Pediatr Res

View full text Add to dashboard Cite

show abstract

“…Furthermore, fresh serum from relatively healthy subjects with CF is usually a more potent stimulator of mucus secretion than age-and sex-matched control sera. These results extend previous observations (2,6,8,11,12,14,16) by demonstrating quantitatively that CF serum augments the release of substances, most likely mucous glycoproteins, from cells of mammalian airways.…”

Section: Discussionsupporting

confidence: 80%

“…Light and electron microscopic examination of epithelium exposed for 15 rnin to 50% human serum demonstrates intact ciliated and goblet cells (unpublished observation). We also were not able to confirm the observation that calcium iontophore A23187 mimics the C F serum effect on mucus secretion by rabbit tracheal epithelium (6). Our observations concerning the time course of the secretory effect of CF serum are generally in keeping with the morphologic observations of Czegledy-Nagy and Sturgess (12) who demonstrated swelling of goblet cells followed by discharge of their contents within 10-20 rnin after exposure to CF serum.…”

Section: Discussionsupporting

confidence: 72%