The biological effect of recombinant humanized collagen on damaged skin induced by UV-photoaging: An in vivo study

Abstract

The application of medical devices to repair skin damage is clinically accepted and natural polymer enjoys an important role in this field, such as collagen or hyaluronic acid, etc. However, the biosafety and efficacy of these implants are still challenged. In this study, a skin damage animal model was prepared by UV-photoaging and recombinant humanized type III collagen (rhCol III) was applied as a bioactive material to implant in vivo to study its biological effect, comparing with saline and uncrosslinked hyaluronic acid (HA). Animal skin conditions were non-invasively and dynamically monitored during the 8 weeks experiment. Histological observation, specific gene expression and other molecular biological methods were applied by the end of the animal experiment. The results indicated that rhCol III could alleviate the skin photoaging caused by UV radiation, including reduce the thickening of epidermis and dermis, increase the secretion of Collagen I (Col I) and Collagen III (Col III) and remodel of extracellular matrix (ECM). Although the cell-material interaction and mechanism need more investigation, the effect of rhCol III on damaged skin was discussed from influence on cells, reconstruction of ECM, and stimulus of small biological molecules based on current results. In conclusion, our findings provided rigorous biosafety information of rhCol III and approved its potential in skin repair and regeneration. Although enormous efforts still need to be made to achieve successful translation from bench to clinic, the recombinant humanized collagen showed superiorities from both safety and efficacy aspects.

Preparation of Recombinant Human Collagen III Protein Hydrogels with Sustained Release of Extracellular Vesicles for Skin Wound Healing

“…The recombinant human collagen III protein (rhCol III) is the product of a re-optimized design gene sequence based on the characteristics and main functional domains of human collagen. In addition, rhCol III could promote skin repair and regeneration [ 27 ]. In the present study, a novel type of hydrogel material was prepared by using rhCol III as the raw material to achieve the sustained release of EVs in skin wounds.…”

Photoprotective effects of sweet potato leaf polyphenols and caffeic acid against UV-induced skin-damage in BALB/C nude mice

“…13 The hydroxyproline (HYP) content in skin tissue reflects the level of collagen fibers in the dermal section and can serve as an indicator of the skin aging stage. 14 Photoaging is a kind of skin aging induced by UV irradiation which is mainly characterized by epidermis and dermis hyperplasia. 14,15 MMPs can be activated by UV induced oxidative stress and inflammatory response.…”

“…14 Photoaging is a kind of skin aging induced by UV irradiation which is mainly characterized by epidermis and dermis hyperplasia. 14,15 MMPs can be activated by UV induced oxidative stress and inflammatory response. 16 Excessive generation of reactive oxygen species (ROS), produced from UV induced oxidative stress, leads to lipid peroxidation and inflammation incidence.…”

Translation of biomaterials from bench to clinic

“…A total of 24 articles [ [4] , [5] , [6] , [7] , [8] , [9] , [10] , [11] , [12] , [13] , [14] , [15] , [16] , [17] , [18] , [19] , [20] , [21] , [22] , [23] , [24] , [25] , [26] , [27] ] are included in this special issue. These articles can be categorized in terms of authors, biomaterials and targeted clinical areas, and article types.…”

“…Several articles in this special issue are on regulatory science and discuss the new standards, tools, and approaches to evaluate the safety, efficacy, quality, and performance of medical products [ 10 , 13 , 17 , 19 ]. This special issue also covers a wide range of article types including research article [ [1] , [2] , [3] , [4] , [5] , [6] , [7] , [8] , [9] , 11 , 12 , 15 , 16 , [18] , [19] , [20] , [23] , [24] , [25] , [26] ], reviews [ 14 , 17 , 21 , 22 , 27 ], perspectives [ 10 , 13 ] and opinion paper [ 18 ].…”