The biological response to three different nanostructures applied on smooth implant surfaces

Jimbo, Ryo; Sotres, Javier; Johansson, Carina B.; Breding, Karin; Currie, Fredrik; Wennerberg, Ann

doi:10.1111/j.1600-0501.2011.02182.x

Cited by 48 publications

(55 citation statements)

References 35 publications

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“…All implants were sandblasted and acid etched (Aadva surface, GC Dental, Tokyo Japan). Half of the implants (HA) were coated with nano-sized HA according to the Promimic HA nano ™ method (Jimbo et al, 2012). The other half of the implants were subjected to only heat treatment in the same manner as the HA implants (HT).…”

Section: Implant Surface Preparationmentioning

confidence: 99%

“…Further, compared to the traditional 'thick' HA coatings, which generated clinical problems (Albrektsson, 1998), the mono-layered thin HA-coating seems to be stable and shows no signs of foreign body reaction (Jimbo et al, 2012). In a previous study observing the gene expression around turned-nano HAcoated implants, the nano HA-coating significantly enhanced osteogenic gene expression while increasing osteoclastic activity suggesting the nano HA is actively involved in the bone formation (Jimbo et al, 2011b).…”

Section: Introductionmentioning

confidence: 99%

“…What is unique about the effect of HA nanocoating is that the stimulatory outcomes are related not only to the topography, but also to the effect of chemistry, which generates a synergetic effect (Jimbo et al, 2012). Further, compared to the traditional 'thick' HA coatings, which generated clinical problems (Albrektsson, 1998), the mono-layered thin HA-coating seems to be stable and shows no signs of foreign body reaction (Jimbo et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…Nanostructures applied to biomaterials have been suggested to contribute to higher grade of osseointegration (Ellingsen et al, 2004), and it has been shown that biology responds sensitively to different nanostructures (Coelho et al, 2011;Jimbo et al, 2012). In fact, Webster and Ahn specified that nanostructures below 100 nm in size are most effective in cellular integration and suggested that these should be differentiated from the so-called submicron structures (Webster and Ahn, 2007).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Nano Hydroxyapatite-coated Implants Improve Bone Nanomechanical Properties

et al. 2012

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Abstract:Nanostructure modification of dental implants has long been sought as a mean to improve osseointegration through enhanced biomimicry of host structures. Several methods have been proposed and demonstrated for creating nanotopographic features; here we described a nanoscale hydroxyapatite (HA) coated implant surface and hypothesized that it will hasten osseointegration and improve its quality relative to non-coated implants. Twenty threaded titanium alloy implants, half prepared with stable HA nanoparticle surface and half grit-blasted, acid-etched, and heat treated (HT), were inserted into rabbit femurs. Preoperatively, the implants were morphologically and topographically characterized. After 3 weeks of healing, the samples were retrieved for histomorphometry. Moreover, the nanomechanical properties of the surrounding bone were evaluated using nanoindentation. While both implants revealed similar bone-to-implant contact, the nanoindentation demonstrated that the tissue quality was significantly enhanced around the HA-coated implants, validating the postulated hypothesis.

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Section: Implant Surface Preparationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Nano Hydroxyapatite-coated Implants Improve Bone Nanomechanical Properties

et al. 2012

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show abstract

“…Recent in vivo studies have shown that nanostructured implant surfaces enhance bone responses, as measured both histologically and biomechanically (Bjursten et al, 2010;Jimbo et al, 2011b). The beneficial effects of these alterations may stem from conversion of the material to a highly bioactive state because of modifications at the nano level (Goransson et al, 2006;Frojd et al, 2011), which is the core concept of the biologically inspired biomimetic engineering (Flammang and Porter, 2011).…”

Section: Introduction Tmentioning

confidence: 99%

Genetic Responses to Nanostructured Calcium-phosphate-coated Implants

et al. 2011

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Nanostructured calcium phosphate (CaP) has been histologically and biomechanically proven to enhance osseointegration of implants; however, conventional techniques were not sufficiently sensitive to capture its biological effects fully. Here, we compared the conventional removal torque (RTQ) evaluation and gene expression in tissues around nanostructured CaP-coated implants, using real-time RT-PCR, with those of uncoated implants, in a rabbit model. At 2 wks, RTQ values were significantly higher, alkaline phosphatase (ALP) expression was significantly higher, and runt-related transcription factor 2 and tumor necrosis factor-α expressions were significantly lower in the coated than in the uncoated implants. This indicates that inflammatory responses were suppressed and osteoprogenitor activity increased around the CaP-coated surface. At 4 wks, although RTQ values did not significantly differ between the 2 groups, ALP and osteocalcin (OCN) were significantly up-regulated in the coated group, indicating progressive mineralization of the bone around the implant. Moreover, an osteoclast marker, adenosine triphosphatase, which indicates acidification of the resorption lacunae, was significantly higher for the coated implants, suggesting gradual resorption of the CaP coating. This study reveals detailed genetic responses to nanostructured CaP-coated implants and provides evidence that the effect of nanotopography is significant during the osseointegration cascade.

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Titanium surfaces with nanotopography modulate cytokine production in cultured human gingival fibroblasts

Schwartz-Filho

Morandini

Ramos-Junior

et al. 2012

J Biomedical Materials Res

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Implant topography is an important factor that influences many cell types. To understand the role of topography in the inflammatory events, we evaluated the response of human gingival fibroblasts (HGFs) by the release pattern of cytokines. HGFs were cultured on Ti discs for 24 and 48 h. Four different surface treatments were used: machining method (turned), blasting followed by an acid-etching method (BAE), oxidative nanopatterning (ON) method, and an association of blasting followed by an acid-etching plus oxidative nanopatterning (BAE+ON) method. Extracellular levels of IL-6, IL-8, transforming growth factor beta (TGF-β), IL-4, and IL-10 were measured by enzyme-linked immunosorbant assay. Increased levels of IL-6 and IL-8 were observed in all surfaces after 24 h which decreased after 48 h. BAE, ON, and BAE+ON surfaces showed a reduction in IL-6 levels compared with the turned after 48 h (p < 0.05). On one hand, IL-8 production was lower in BAE+ON in comparison to the turned surface (p < 0.05). On the other hand, IL-4 showed increased levels with 48 h, which were significantly different between turned, BAE, and ON surfaces, but not with BAE+ON. Additionally, TGF-β and IL-10 production were not detected. This study indicates that nanotopography might be important in the modulation of the inflammatory response in cultured HGFs.

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The biological response to three different nanostructures applied on smooth implant surfaces

Abstract: The three different CaP coatings with nanostructures on the implant surfaces had enhancing effects on osseointegration. Along with the surface nanotopography, the CaP chemistry might have influenced the biological outcomes.

Cited by 48 publications

References 35 publications

Nano Hydroxyapatite-coated Implants Improve Bone Nanomechanical Properties

Nano Hydroxyapatite-coated Implants Improve Bone Nanomechanical Properties

Genetic Responses to Nanostructured Calcium-phosphate-coated Implants

Titanium surfaces with nanotopography modulate cytokine production in cultured human gingival fibroblasts

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