“…Physical transport mechanisms, including oxygen transport, nutrients distribution, and gas exchange facilitated by flowing liquid within vascular networks and the surrounding interstitial spaces are widely acknowledged as critical for tissue development and for shaping the overall architecture of vascular networks within tissues. 1,2 These flow processes result in the generation of varying shear stresses within Page 1 of 19 Lab on a Chip 2 multi-sized vessels, which are governed by the pulsating heart in living organisms, as investigated using models such as chick 3 , zebrafish 4 , and frog embryos. 5 Conversely, within developing vessels of engineered tissues in for instance microfluidic chips, these shear stresses are regulated by the pressure driven flows generated by micropumps.…”