The Biosensor based on electrochemical dynamics of fermentation in yeast Saccharomyces Cerevisiae

Abstract

The zymase activity of the yeast Saccharomyces cerevisiae is sensitive to environmental parameters and is therefore used as a microbiological sensor for water quality assessment, ecotoxicological characterization or environmental monitoring. Comparing to bacterial bioluminescence approach, this method has no toxicity, excludes usage of genetically modified microorganisms, and enables low-cost express analysis. This work focuses on measuring the yeast fermentation dynamics based on multichannel pressure sensing and electrochemical impedance spectroscopy (EIS). Measurement results are compared with each other in terms of accuracy, reproducibility and ease of use in the field conditions. It has been shown that EIS provides more information about ionic dynamics of metabolic processes and requires less complex measurements. The conducted experiments demonstrated the sensitivity of this approach for assessing biophotonic phenomena, nonchemical water treatments and impact of environmental stressors.

Device for measuring the plant physiology and electrophysiology

“…for optimization of production or fast tests of genetic hybrids, and involved different sensors and computational units. Next developmental steps have been done within the FloraRobotica [2] and BioHybrids projects [3] with focus on different sensors and physiological processes in plants [4], [5].…”