The biosynthesis and metabolism of oestrogens in the rat. 2. The conversion of [4-14C]testosterone into [14C]oestradiol-17<i>β</i> by the ovary after whole-body irradiation

Stitch, S. R.; Oakey, R. E.; Eccles, Sheila S.

doi:10.1042/bj0880076

Cited by 3 publications

(2 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Oestrogens have been identified after incubation of rat ovarian tissue with radioactive progesterone (Weisz & Lloyd, 1965) and testosterone (Stitch, Oakey & Eccles, 1963).…”

Section: Discussionmentioning

confidence: 99%

REGULATION OF PRODUCTION IN VITRO OF 5α-Androstane-3α,17β-Diol IN THE IMMATURE RAT OVARY

Springer¹,

Eckstein²

1971

Journal of Endocrinology

View full text Add to dashboard Cite

The pattern of steroid synthesis in vitro in ovarian homogenate of immature rats was investigated with [7\g=a\-3H]pregnenolone as radioactive precursor. The metabolites, progesterone, androstenedione, testosterone and 5\g=a\-androstane-3\g=a\,17\g=b\-diol were obtained in radiochemically pure form. The steroids were identified by fractionation, partition column and thin-layer chromatography, derivative formation and by crystallization to constant activity. The steroidogenic patterns at the ages of 21 and of 31 days were found to be similar, but different from the pattern reported for adult rat ovaries. At 34 days of age, 1 day before the onset of puberty, the percentage radioactivity converted to progesterone was about ten times higher than that at 31 days. The production in vitro of 5\g=a\-androstane-3\g=a\,17\g=b\-diolby ovarian homogenates of 34-day-old rats was shown to depend on the concentration of pregnenolone in the incubation mixture. These findings are discussed in relation to the mechanism of the onset of puberty in the female mammal.

show abstract

“…Oestrogens have been identified after incubation of rat ovarian tissue with radioactive progesterone (Weisz & Lloyd, 1965) and testosterone (Stitch, Oakey & Eccles, 1963).…”

Section: Discussionmentioning

confidence: 99%

REGULATION OF PRODUCTION IN VITRO OF 5α-Androstane-3α,17β-Diol IN THE IMMATURE RAT OVARY

Springer¹,

Eckstein²

1971

Journal of Endocrinology

View full text Add to dashboard Cite

show abstract

“…Conversion, which has been demonstrated in rat 37 and human 38 ovary slices in vitro, has desirable physiological consequences across animal species, ranging from acceleration of gonadal differentiation and stimulation of spermatogenesis in prepubertal sea bass 39 and modulation of respiratory long-term facilitation in male rats 40 to neuroprotection in humans. 41 The effects of dose and age on the conversion of injected T to E2 were studied in humans in detail.…”

Section: The Hypothesis and Its Backgroundmentioning

confidence: 99%

Modulation of Tryptophan and Serotonin Metabolism as a Biochemical Basis of the Behavioral Effects of Use and Withdrawal of Androgenic-Anabolic Steroids and Other Image- and Performance-Enhancing Agents

Badawy

2018

Int J�Tryptophan�Res

View full text Add to dashboard Cite

Modulation of tryptophan (Trp) metabolism may underpin the behavioral effects of androgenic-anabolic steroids (AAS) and associated image and performance enhancers. Euphoria, arousal, and decreased anxiety observed with moderate use and exercise may involve enhanced cerebral serotonin synthesis and function by increased release of albumin-bound Trp and estrogen-mediated liver Trp 2,3-dioxygenase (TDO) inhibition and enhancement of serotonin function. Aggression, anxiety, depression, personality disorders, and psychosis, observed on withdrawal of AAS or with use of large doses, can be caused by decreased serotonin synthesis due to TDO induction on withdrawal, excess Trp inhibiting the 2 enzymes of serotonin synthesis, and increased cerebral levels of neuroactive kynurenines. Exercise and excessive protein and branched-chain amino acid intakes may aggravate the effects of large AAS dosage. The hypothesis is testable in humans and experimental animals by measuring parameters of Trp metabolism and disposition and related metabolic processes.

show abstract

Hormones and Systemic Effects

Altman

1970

Tissues and Body Fluids

View full text Add to dashboard Cite

The biosynthesis and metabolism of oestrogens in the rat. 2. The conversion of [4-14C]testosterone into [14C]oestradiol-17β by the ovary after whole-body irradiation

Cited by 3 publications

References 14 publications

REGULATION OF PRODUCTION IN VITRO OF 5α-Androstane-3α,17β-Diol IN THE IMMATURE RAT OVARY

REGULATION OF PRODUCTION IN VITRO OF 5α-Androstane-3α,17β-Diol IN THE IMMATURE RAT OVARY

Modulation of Tryptophan and Serotonin Metabolism as a Biochemical Basis of the Behavioral Effects of Use and Withdrawal of Androgenic-Anabolic Steroids and Other Image- and Performance-Enhancing Agents

Hormones and Systemic Effects

Contact Info

Product

Resources

About