The Biosynthesis of D-1,2,4-Butanetriol From d-Arabinose With an Engineered Escherichia coli

Abstract

D-1,2,4-Butanetriol (BT) has attracted much attention for its various applications in energetic materials and the pharmaceutical industry. Here, a synthetic pathway for the biosynthesis of BT from d-arabinose was constructed and optimized in Escherichia coli. First, E. coli Trans1-T1 was selected for the synthesis of BT. Considering the different performance of the enzymes from different organisms when expressed in E. coli, the synthetic pathway was optimized. After screening two d-arabinose dehydrogenases (ARAs), two d-arabinonate dehydratases (ADs), four 2-keto acid decarboxylases (ADXs), and three aldehyde reductases (ALRs), ADG from Burkholderia sp., AraD from Sulfolobus solfataricus, KivD from Lactococcus lactis IFPL730, and AdhP from E. coli were selected for the bio-production of BT. After 48 h of catalysis, 0.88 g/L BT was produced by the recombinant strain BT5. Once the enzymes were selected for the pathway, metabolic engineering strategy was conducted for further improvement. The final strain BT5ΔyiaEΔycdWΔyagE produced 1.13 g/L BT after catalyzing for 48 h. Finally, the fermentation conditions and characteristics of BT5ΔyiaEΔycdWΔyagE were also evaluated, and then 2.24 g/L BT was obtained after 48 h of catalysis under the optimized conditions. Our work was the first report on the biosynthesis of BT from d-arabinose which provided a potential for the large-scale production of d-glucose-based BT.

Efficient production of 1,2,4-butanetriol from corn cob hydrolysate by metabolically engineered Escherichia coli

“…Besides xylose, xylonolactone and xylonate, XylB, XylC and XylD from C. crescentus are also active on arabinose, arabinolactone and arabinonate, respectively [ 37 ]. KdcA from L. lactis catalyzes the decarboxylation of both 2-keto-3-deoxy-xylonate and 2-keto-3-deoxy-arabinonate [ 38 ]. Although the catabolic genes for arabinose utilization were not deleted in E. coli BT-10, these four heterologous enzymes and endogenous alcohol dehydrogenase YqhD may efficiently redirect the metabolic flux of arabinose from central metabolism to 1,2,4-BT production (Fig.…”

Evaluating Cefoperazone-Induced Gut Metabolic Functional Changes in MR1-Deficient Mice

“…Xylonate/arabinonate (sugar acids) is known for being an energy source for some bacteria containing xylose/arabinonate dehydrogenase, including G. oxydans , pseudomonas putida , and E. coli [ 23 , 24 ]. Increases in these carbohydrates—which could be the essential energy source for pathogens, such as C. difficile —might confer CDI susceptibility in WT mice.…”

Development of a New 1,2,4-butanetriol Biosynthesis Pathway in an Engineered Homoserine-producing Strain of Escherichia coli

“…Bamba et al (2019) [10] engineered Saccharomyces cerevisiae to produce 1.7 g/L of BT from 10 g/L xylose with a molar yield of 24.5%, after the enhancement of uptaking ability of Fe to improve the activity of the rate-limiting enzyme, D-xylonate dehydratase (XylD), and screen the optimal 2ketoacid decarboxylase. Besides this, Wang et al (2022) [11] constructed a synthetic pathway for the biosynthesis of BT from D-arabinose in E. coli, achieving 2.24 g/L BT after 48 h of catalysis under the optimized fermentation conditions.…”