The biosynthesis of glucose containing insect lipid linked oligosaccharide and its possible role in glycoprotein assembly

Allué, Luis Alberto Quesada

doi:10.1007/bf00225287

Cited by 13 publications

(6 citation statements)

References 26 publications

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“…In all cases, all types of Asn-tinked oligosaccharides are believed to be the product of a common precursor glycan transferred cotranslationally from dolichyl-P-P-glycan [18,22,32,43]. Complex oligosaccharides are generated after the trimming in the transport pathway [19,32].…”

Section: Introductionmentioning

confidence: 99%

Investigation of the role of glycans for the biological activity of Semliki Forest virus grown in Aedes albopictus cells using inhibitors of asparagine-linked oligosaccharides trimming

Naim

Koblet

1988

Archives of Virology

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The effects of N-linked-oligosaccharide-processing inhibitors on the formation of Semliki Forest virus (SFV) in C6/36 Aedes albopictus cells were investigated. The glycosidase inhibitors deoxynojirimycin, deoxymannojirimycin and swainsonine prevented the formation of Endo-H resistant structures, but had little effect on virus formation and on the biological activities of the virus. Tunicamycin greatly inhibited virus formation, but had little effect on cell-cell fusion from within and the cleavage of p 62. These results indicate that correct glycosylation is not a prerequisite for biological activities of SFV, whereas glycosylation per se is needed for virus production.

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Section: Introductionmentioning

confidence: 99%

Investigation of the role of glycans for the biological activity of Semliki Forest virus grown in Aedes albopictus cells using inhibitors of asparagine-linked oligosaccharides trimming

Naim

Koblet

1988

Archives of Virology

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“…It has become evident in recent years that glycosylation of asparagine residues in eukaryotic cell proteins involves dolichol pyrophosphate (DolPP)-bound oligosaccharides as intermediates (1). It has been reported that in animal tissues (2,3), yeasts (4)(5)(6), and probably also in insects (7) and plants (8), the oligosaccharide transferred from the lipid derivative to proteins is composed of two N-acetylglucosamine, nine mannose, and three glucose residues. A report by Lehle suggests that in yeast the oligosaccharides containing two or three glucose residues are equally transferred to protein (9).…”

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confidence: 99%

Pathway of protein glycosylation in the trypanosomatid Crithidia fasciculata.

Parodi¹,

Allué²,

Cazzulo³

1981

Proc. Natl. Acad. Sci. U.S.A.

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Cells of the insect parasite Crithidia fasciculata incubated with ['4C]glucose were found to possess only one lipidbound oligosaccharide with solubility in chloroform/methanol/ water mixtures and net charge similar to the charges of dolichol pyrophosphate derivatives. The saccharide moiety could be released from lipid by mild acid hydrolysis. Several enzymatic and chemical treatments of the oligosaccharide indicated that the latter had the structure Mana-+Mana---Mana--[Mana-+ Mana-+Man(al--6)]Man--GlcNAc(l1--4)GlcNAc. Two labeled oligosaccharides were liberated from proteins by a sequential treatment with a protease and endo-13-N-acetylglucosaminidase H. One ofthe protein-bound oligosaccharides had the same structure as the lipid-linked compound, whereas in the second oligosaccharide some mannose residues had been replaced by galactose units, but both compounds migrated as did a Man7GlcNAc standard. These were the largest oligosaccharides obtained even after short labeling periods. It is suggested that glycosylation ofproteins in the protozoan Crithidiafasciculata does not involve glucosylated lipid-bound oligosaccharides as intermediates. It has become evident in recent years that glycosylation of asparagine residues in eukaryotic cell proteins involves dolichol pyrophosphate (DolPP)-bound oligosaccharides as intermediates (1). It has been reported that in animal tissues (2, 3), yeasts (4-6), and probably also in insects (7) and plants (8), the oligosaccharide transferred from the lipid derivative to proteins is composed of two N-acetylglucosamine, nine mannose, and three glucose residues. A report by Lehle suggests that in yeast the oligosaccharides containing two or three glucose residues are equally transferred to protein (9). However, no evidence was presented indicating that the oligosaccharides used in the assay had the same specific activity.The protein-bound oligosaccharides are then processed by loss of some of their monosaccharide constituents and addition ofother residues directly from the respective sugar nucleotides. We here report evidence suggesting that in the protozoan Crithidta fasciculata, an insect obligate parasite, the oligosaccharide transferred to protein contains two N-acetylglucosamine and seven mannose residues and that the oligosaccharide may be processed once bound to protein.MATERIALS AND METHODS Materials.[14C]Glucose (284 Ci/mol; 1 Ci = 3.7 X 1010 becquerels) was from New England Nuclear. Jack bean a-mannosidase type III and Streptomyces griseus protease type VI were purchased from Sigma and endo-,B3N-acetylglucosaminidase H (endo H), from Miles. Isolation of Lipid-Bound Oligosaccharides. C. fasciculata cells (Anopheles isolate ATCC 11745) were grown in the medium described by Bacchi et aL (10) without agar at 28°C. Six hundred milliliters of culture containing about 2 g of cells (logarithmic phase) were cooled on ice and centrifuged at 2500 X g for 10 min at 4°C. The cell pellet was resuspended in 30 ml of ice-cold minimal Eagle's medium without glucose but containing 5...

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“…Dolichol-linked sugars are intermediates in the glycosylation of some eukaryotic and viral proteins (Schachter, 1978;Parodi & Leloir, 1979). We previously reported biosynthesis both in vivo and in vitro of some insect dolichyl derivatives (Quesada- Allue et al, 1976a,b;Quesada-Allue & Belocopitow, 1978), which also seemed to be involved in the glycosylation of insect proteins (Quesada-Allue, 1978, 1980Butters & Hughes, 1980), thus confirming that this mechanism is a general feature of eukaryotic organisms.…”

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confidence: 59%

“…Vol. 198 Experimental The analytical procedures used in the present paper have been described previously (Quesada-Allue & Belocopitow, 1978;Quesada-Allue, 1980). The same results were obtained with different batches ofArtemia cysts.…”

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confidence: 98%

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Early dolichyl sugar synthesis during differentiation of non-growing brine-shrimp (Artemia salina) embryos

Quesada-Allue¹

1981

Biochemical Journal

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The biosynthesis of glucose containing insect lipid linked oligosaccharide and its possible role in glycoprotein assembly

Cited by 13 publications

References 26 publications

Investigation of the role of glycans for the biological activity of Semliki Forest virus grown in Aedes albopictus cells using inhibitors of asparagine-linked oligosaccharides trimming

Investigation of the role of glycans for the biological activity of Semliki Forest virus grown in Aedes albopictus cells using inhibitors of asparagine-linked oligosaccharides trimming

Pathway of protein glycosylation in the trypanosomatid Crithidia fasciculata.

Early dolichyl sugar synthesis during differentiation of non-growing brine-shrimp (Artemia salina) embryos

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