The biosynthesis of plant alkaloids and nitrogenous microbial metabolites (1997 to 1998)

Herbert, Richard B.

doi:10.1039/a809393h

Cited by 33 publications

(14 citation statements)

References 110 publications

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“…It stimulates the accumulation of the secondary metabolites such as tropane alkaloids in solanaceous plants ( 25 ). The use of elicitors or phytohormones, such as jasmonate and its volatile derivative methyl jasmonate ( 26 , 27 ), is a possible approach for increasing tropane alkaloid biosynthesis in Datura species ( 25 , 28 ). However, plant growth is then inhibited ( 25 , 29 , 30 ).…”

Section: Introductionmentioning

confidence: 99%

The Desirability Optimization Methodology; a Tool to Predict Two Antagonist Responses in Biotechnological Systems: Case of Biomass Growth and Hyoscyamine Content in Elicited Datura starmonium Hairy Roots

Amdoun¹,

Khelifi²,

Khelifi-Slaoui³

et al. 2018

ijbiotech

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BackgroundThe use of the desirability function approach combined with the response surface methodology (RSM), also called Desirability Optimization Methodology (DOM), has been successfully applied to solve medical, chemical, and technological questions. It is particularly efficient for the determination of the optimal conditions in natural or industrial processes involving different factors leading to the antagonist responses.ObjectivesSurprisingly, DOM has never been applied to the research programs devoted to the study of plant responses to the complex environmental changes, and thus to biotechnological questions.Materials and MethodsIn this article, DOM is used to study the response of Datura stramonium hairy roots (HRs), obtained by genetic transformation with Agrobacterium rhizogenes A4 strain, subjected to the jasmonate treatments.ResultsAntagonist effects on the growth and tropane alkaloid biosynthesis are confirmed. With a limited number of experimental conditions, it is shown that 0.06 mM jasmonic acid (JA) applied for 24 h leads to an optimal compromise. Hyoscyamine levels increase by up to 290% after 24 h and this treatment does not significantly inhibit biomass growth.ConclusionsIt is thus demonstrated that the use of DOM can efficiently - with a minimized number of replicates - leads to the optimization of the biotechnological processes.

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Section: Introductionmentioning

confidence: 99%

The Desirability Optimization Methodology; a Tool to Predict Two Antagonist Responses in Biotechnological Systems: Case of Biomass Growth and Hyoscyamine Content in Elicited Datura starmonium Hairy Roots

Amdoun¹,

Khelifi²,

Khelifi-Slaoui³

et al. 2018

ijbiotech

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“…Besides, PrnF was also demonstrated to stimulate (i) chorinaton by PrnA and PrnC activity in PRN synthesis (Richard 2003), (ii) halogenation of aromatic compounds by RebH and ThdH in rebecamycin, thienodolin (Sanchez et al 2002;Sebold et al 2006) and (iii) bromination by BrvH in marine metagenomes (Neubauer et al 2018) and reported to catalyse the reduction of avin such as avin mononucleotide, avin adenine dinucleotide and ribo avin by NAD(P)H to form reduced avin which is required to activate oxygen by the terminal oxygenase (Hammer et al 1997). At present, prnF was identi ed via sequence analysis only from Pseudomonas uorescence Pf-5 and characterized to supply reduced avin to prnD oxygenase component to function in PRN synthesis (Hammer et al 1997).…”

Section: Introductionmentioning

confidence: 94%

Pyrrolnitrin Biosynthesis From Rhizospheric Serratia Spp. With Antifungal Activity and Binding Interactions of PrnF With Ligands

Pawar

Chaudhari

2020

Preprint

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Pyrrolnitrin (PRN) from rhizobacteria displays a key role in biocontrol of phytopathogenic fungi in rhizospheric soil. Therefore, different rhizospheric soils were investigated for the prevalence of PRN producer in minimal salt (MS) medium containing tryptophan (0.2 M NaCl; pH 8) using three successive enrichments. Of 12% isolates, only five bacterial strains had shown PRN secretion, screened with Thin Layer Chromatography (Rf 0.8) and antifungal activity (27 mm) against phytopathogen. The phenetic and 16S rRNA sequence revealed the close affiliation of isolates (KMB, M-2, M-11, TW3, and TO2) to Stenotrophomonas rhizophila (KY800458), Enterobacter spp. (KY800455), Brevibacillus parabrevis (KY800454), Serratia marcescens (KY800456) and Serratia nemtodiphila (KY800457). Purified compound from isolates was characterised using UV, IR, HPLC, LCMS and GCMS as PRN. However, BLASTn hit of prn gene sequences from both Serratia species showed 99% similarity with NADPH dependent FMN reductase component (prnF). The homology protein model of prnF was developed from translated sequence of S. marcescens TW3 with chromate reductase of Escherichia coli K-12. Docking with FMN and NADPH was performed. The study demonstrated the possible role of prnF NADPH dependent FMN reductases in prnD for supply of reduced flavin in rhizobacterial strain of Serratia spp. which may pave a way to understand PRN production.

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“…They are characterized by their bitter taste and can be found in glycosylated, methylated, and hydroxylated forms, in which nitrogen (N) is present as a characteristic element of their structure [160]. Thus, alkaloids can be classified according to their structural characteristics and their biosynthesis process, being named as true alkaloids, pseudoalkaloids, protoalkaloids [161].…”

Section: Alkaloidsmentioning

confidence: 99%

The Compositional Aspects of Edible Flowers as an Emerging Horticultural Product

et al. 2021

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Edible flowers are becoming very popular, as consumers are seeking healthier and more attractive food products that can improve their diet aesthetics and diversify their dietary sources of micronutrients. The great variety of flowers that can be eaten is also associated with high variability in chemical composition, especially in bioactive compounds content that may significantly contribute to human health. The advanced analytical techniques allowed us to reveal the chemical composition of edible flowers and identify new compounds and effects that were not known until recently. Considering the numerous species of edible flowers, the present review aims to categorize the various species depending on their chemical composition and also to present the main groups of compounds that are usually present in the species that are most commonly used for culinary purposes. Moreover, special attention is given to those species that contain potentially toxic or poisonous compounds as their integration in human diets should be carefully considered. In conclusion, the present review provides useful information regarding the chemical composition and the main groups of chemical compounds that are present in the flowers of the most common species.

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The biosynthesis of plant alkaloids and nitrogenous microbial metabolites (1997 to 1998)

Cited by 33 publications

References 110 publications

The Desirability Optimization Methodology; a Tool to Predict Two Antagonist Responses in Biotechnological Systems: Case of Biomass Growth and Hyoscyamine Content in Elicited Datura starmonium Hairy Roots

The Desirability Optimization Methodology; a Tool to Predict Two Antagonist Responses in Biotechnological Systems: Case of Biomass Growth and Hyoscyamine Content in Elicited Datura starmonium Hairy Roots

Pyrrolnitrin Biosynthesis From Rhizospheric Serratia Spp. With Antifungal Activity and Binding Interactions of PrnF With Ligands

The Compositional Aspects of Edible Flowers as an Emerging Horticultural Product

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