The biosynthesis of squalene and sterols by the adipose tissue of rat, sheep and man

Abstract

1. The subcutaneous and omental adipose tissue of man, the epididymal fat pads of the rat and the fat tail of the Syrian sheep incorporate mevalonic acid into non-saponifiable lipids. 2. Time studies showed that the rates of decarboxylation of mevalonic acid and synthesis of non-saponifiable lipids slightly decline after 20min. but subsequently remain linear for 6hr. 3. About one-half of the incorporated radioactivity in the non-saponifiable lipids was in squalene, 20% in lanosterol and cholesterol, and the remainder in unidentified substances.

Effects of FoxO4 overexpression on cholesterol biosynthesis, triacylglycerol accumulation, and glucose uptake

“…The ability of FoxO4 to inhibit cholesterol biosynthesis is likely to be most impacted in cells that are actively synthesizing cholesterol, such as in hepatocytes. In adipocytes, however, the effi ciency of cholesterol biosynthesis is very low due to the accumulation of methylsterols ( 27,28 ). Consistent with these observations was the lack of a detectable effect on cholesterol biosynthesis in transgenic mice overexpressing FoxO4 in adipocytes (12) (data not shown).…”

Induction of β-Galactosidase in Lactobacillus plantarum

“…Both were kept at 35 C, and every 30 min 1 ml was drawn from each and supplemented with [1-14C ]galactose (1 MCi) and incubated at 35 C for 1 h, after which 0.4 ml of 4 N H,SO4 was added to terminate the reaction. 14CO0 was collected and counted as previously described (6).…”

“…The anionic compounds retained were then eluted with 0.5 N HCl. The eluate was neutralized with 1 N NaOH, and 0.1-ml samples were counted as previously described (6).…”