The biosynthetic pathway of ecdysone: Studies with vitellogenic ovaries of Locusta migratoria (orthoptera)

Hétru, Charles; Kappler, Christine; Hoffmann, Jules A.; Nearn, Roland H.; Luu, Bang; Horn, D. H. S.

doi:10.1016/0303-7207(82)90006-5

Cited by 51 publications

(17 citation statements)

References 31 publications

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“…The ovary is one of the sources of ecdysteroids in female adult insects. In cockroaches and locusts it has been shown that the follicle cells can synthesize and secrete ecdysone [32]–[34]. In Drosophila , in vitro synthesis and secretion of ecdysteroids has also been described in the ovary [35], [36].…”

Section: Resultsmentioning

confidence: 99%

Scavenger Receptors Mediate the Role of SUMO and Ftz-f1 in Drosophila Steroidogenesis

et al. 2013

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SUMOylation participates in ecdysteroid biosynthesis at the onset of metamorphosis in Drosophila melanogaster. Silencing the Drosophila SUMO homologue smt3 in the prothoracic gland leads to reduced lipid content, low ecdysone titers, and a block in the larval–pupal transition. Here we show that the SR-BI family of Scavenger Receptors mediates SUMO functions. Reduced levels of Snmp1 compromise lipid uptake in the prothoracic gland. In addition, overexpression of Snmp1 is able to recover lipid droplet levels in the smt3 knockdown prothoracic gland cells. Snmp1 expression depends on Ftz-f1 (an NR5A-type orphan nuclear receptor), the expression of which, in turn, depends on SUMO. Furthermore, we show by in vitro and in vivo experiments that Ftz-f1 is SUMOylated. RNAi–mediated knockdown of ftz-f1 phenocopies that of smt3 at the larval to pupal transition, thus Ftz-f1 is an interesting candidate to mediate some of the functions of SUMO at the onset of metamorphosis. Additionally, we demonstrate that the role of SUMOylation, Ftz-f1, and the Scavenger Receptors in lipid capture and mobilization is conserved in other steroidogenic tissues such as the follicle cells of the ovary. smt3 knockdown, as well as ftz-f1 or Scavenger knockdown, depleted the lipid content of the follicle cells, which could be rescued by Snmp1 overexpression. Therefore, our data provide new insights into the regulation of metamorphosis via lipid homeostasis, showing that Drosophila Smt3, Ftz-f1, and SR-BIs are part of a general mechanism for uptake of lipids such as cholesterol, required during development in steroidogenic tissues.

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Section: Resultsmentioning

confidence: 99%

Scavenger Receptors Mediate the Role of SUMO and Ftz-f1 in Drosophila Steroidogenesis

et al. 2013

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“…There is ample evidence for ecdysteroidogenesis by ovaries of other insect species (Zhu et al, 1983;Lanot et al, 1989;, and presumably, this occurs in the ovaries of A. aegypti. Definitive evidence for ecdysteroidogenesis in ovaries and other tissues in A. aegypti will be attained when the conversion of a radiolabeled sterol precursor into ecdysone or 20-hydroxyecdsone is demonstrated (Hetru et al, 1982;Dolle et al, 1990;.…”

Section: Ecdysteroidogenesis and Gene Expression In Other Female Tissmentioning

confidence: 98%

Expression of genes encoding proteins involved in ecdysteroidogenesis in the female mosquito, Aedes aegypti

Sieglaff¹,

Duncan²,

Brown³

2005

Insect Biochemistry and Molecular Biology

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“…These molecules, which had a relatively low specific activity ( <1 Ci/mmol), were used in a series of conversion experiments with various insect species, i.e. Calliphora stygia (Galbraith et al, 1973 and1975;Horn et al, 1976;Faux et al, 1979), Manduca sexta (Bollenbacher et al, 1977 and and Locusta migratoria (Hetru et al, 1982).…”

Section: Introductionmentioning

confidence: 99%

Conversion of a Radiolabelled Putative Ecdysone Precursor, 2,22,25-Trideoxyecdysone (5β-Ketodiol) in Larvae and Pupae ofCalliphora vicina

Meister

Brandtner

Koolman

et al. 1987

International Journal of Invertebrate Reproduction and Developm

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When a tritiated ecdysone precursor, 2,22,25-trideoxyecdysone (5,8-ketodiol) was injected into larvae and pupae of Calliphora vicina, it was efficiently converted into 20-hydroxyecdysone. Injections into the anterior and posterior compartments of neck-ligated larvae have shown that both parts can hydroxylate the 5,8-ketodiol at C-25, C-22 and C-2 to form ecdysone which was readily hydroxylated at C-20 to form 20-hydroxyecdysone. When incubated with larval brain-ring gland complexes, 5,8-ketodiol was converted to ecdysone as well as to other metabolites, such as 22,25-dideoxyecdysone and 2-deoxyecdysone. Other larval tissues, namely the fat body, Malpighian tubules, gut and carcass are also able to transform 5,8-ketodiol into ecdysone in vitro, which explains the conversion of the 5Q-ketodiol injected into the abdominal portions of neck-ligated larvae. The large tissue distribution of the enzyme systems involved in the terminal hydroxylations of ecdysteroid biosynthesis in discussed in view of the results obtained in this and other insect orders. ecdysone biosynthesis, ring gland, Calliphora, blowfly, radio-labelled 5,8-ketodiol 0168-8170/87/$03.50 1Cl1987 Balaban

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The biosynthetic pathway of ecdysone: Studies with vitellogenic ovaries of Locusta migratoria (orthoptera)

Cited by 51 publications

References 31 publications

Scavenger Receptors Mediate the Role of SUMO and Ftz-f1 in Drosophila Steroidogenesis

Scavenger Receptors Mediate the Role of SUMO and Ftz-f1 in Drosophila Steroidogenesis

Expression of genes encoding proteins involved in ecdysteroidogenesis in the female mosquito, Aedes aegypti

Conversion of a Radiolabelled Putative Ecdysone Precursor, 2,22,25-Trideoxyecdysone (5β-Ketodiol) in Larvae and Pupae ofCalliphora vicina

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