The BIR and BIR-like domains of Bombyx mori nucleopolyhedrovirus IAP2 protein are required for efficient viral propagation

Ito, Hanako; Bando, Hideaki; Shimada, Toru; Katsuma, Susumu

doi:10.1016/j.bbrc.2014.10.132

Cited by 14 publications

(5 citation statements)

References 24 publications

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“…Previous studies have indicated that the deletion of either the BmNPV iap 1 or iap 2 gene can result in defects in viral replication (Katsuma et al ., ; Ono et al ., ; Ito et al ., ). Here, we found that the overexpression of either the BmNPV iap 1 or iap 2 gene can promote BmNPV proliferation (Fig.…”

Section: Discussionmentioning

confidence: 97%

Evolutionary and functional analyses of the interaction between the Bombyx mori inhibitor of apoptosis (IAP) and nucleopolyhedrovirus IAPs

et al. 2019

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As an important insect immune response, apoptosis plays a critical role in the interaction between baculoviruses and insect hosts. Previous reports have identified inhibitor of apoptosis (IAP) proteins in both insects and baculoviruses, but the relationship between these proteins is still not clearly understood. Here, we found that insect IAP proteins were clustered with baculovirus IAP3, suggesting that the baculovirus iap3 gene might be derived from the Lepidoptera or Diptera. We demonstrated that Bombyx mori inhibitor of apoptosis (Bmiap) gene had an inhibitory effect on apoptosis in silkworm cells. Further analysis of the effects of Bmiap genes on the proliferation of B. mori nucleopolyhedrovirus (BmNPV) showed that both the Bmiap and BmNPV iap genes increased BmNPV proliferation after BmNPV infected silkworm cells. Our results also indicated that BmNPV IAP1 and IAP2 directly interacted with BmIAP in silkworm cells, implying that the Bmiap gene might be hijacked by BmNPV iap genes during BmNPV infection. Taken together, our results provide important insights into the functional relationships of iap genes, and improve our knowledge of apoptosis in baculoviruses and insect hosts.

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Section: Discussionmentioning

confidence: 97%

Evolutionary and functional analyses of the interaction between the Bombyx mori inhibitor of apoptosis (IAP) and nucleopolyhedrovirus IAPs

et al. 2019

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“…Deletion of the BmNPV iap-1 homolog resulted in a virus that replicated normally in cultured cells, but exhibited a small (15 h) delay in the time required to kill insect larvae [51]. Meanwhile, deletion of the BmNPV iap-2 homolog had an even stronger effect, causing a 100-fold decrease in replication in cell culture as well as a delay in lethal time [52,53]. In contrast, mutation of AcMNPV iap-1 or iap-2 had no apparent effect on the ability of the virus to replicate in several cell lines [42].…”

Section: Do Baculovirus Iaps Have Other Functions Besides Regulating mentioning

confidence: 96%

Viral IAPs, then and now

Clem

2015

Seminars in Cell & Developmental Biology

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“…Intuitively, the anti-apoptotic activities of BIRC3 may play contributory roles in the early stage of MERS-CoV replication. Moreover, a recent study demonstrated that BIRC3 is required for efficient propagation of the baculovirus Bombyx mori nucleopolyhedrovirus through an unknown mechanism 11 . Further study will be required to dissect the function(s) of BIRC3 in MERS-CoV replication.…”

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confidence: 99%

MERS coronavirus induces apoptosis in kidney and lung by upregulating Smad7 and FGF2

et al. 2016

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Middle East respiratory syndrome coronavirus (MERS-CoV) causes sporadic zoonotic disease and healthcare-associated outbreaks in human. MERS is often complicated by acute respiratory distress syndrome (ARDS) and multi-organ failure(1,2). The high incidence of renal failure in MERS is a unique clinical feature not often found in other human coronavirus infections(3,4). Whether MERS-CoV infects the kidney and how it triggers renal failure are not understood(5,6). Here, we demonstrated renal infection and apoptotic induction by MERS-CoV in human ex vivo organ culture and a nonhuman primate model. High-throughput analysis revealed that the cellular genes most significantly perturbed by MERS-CoV have previously been implicated in renal diseases. Furthermore, MERS-CoV induced apoptosis through upregulation of Smad7 and fibroblast growth factor 2 (FGF2) expression in both kidney and lung cells. Conversely, knockdown of Smad7 effectively inhibited MERS-CoV replication and protected cells from virus-induced cytopathic effects. We further demonstrated that hyperexpression of Smad7 or FGF2 induced a strong apoptotic response in kidney cells. Common marmosets infected by MERS-CoV developed ARDS and disseminated infection in kidneys and other organs. Smad7 and FGF2 expression were elevated in the lungs and kidneys of the infected animals. Our results provide insights into the pathogenesis of MERS-CoV and host targets for treatment.

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The BIR and BIR-like domains of Bombyx mori nucleopolyhedrovirus IAP2 protein are required for efficient viral propagation

Cited by 14 publications

References 24 publications

Evolutionary and functional analyses of the interaction between the Bombyx mori inhibitor of apoptosis (IAP) and nucleopolyhedrovirus IAPs

Evolutionary and functional analyses of the interaction between the Bombyx mori inhibitor of apoptosis (IAP) and nucleopolyhedrovirus IAPs

Viral IAPs, then and now

MERS coronavirus induces apoptosis in kidney and lung by upregulating Smad7 and FGF2

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