The Bone Marrow as a Potential Receptor Site for Pancreatic Islet Grafts

Salazar-Bañuelos, Anastasio; Wright, James R.; Sigalet, David L.; Benı́tez-Bribiesca, Luis

doi:10.1016/j.arcmed.2007.09.004

Cited by 20 publications

(11 citation statements)

References 7 publications

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“…Transplantation of bone marrow derived cells into diabetic mice can reducehyperglycemia through proliferation of β-cells, differentiation of pancreatic stem cells to contribute to the rege- neration of β-cells [28,29,43,44], and improve islet graft function by promoting graft vascularization [31,45]. Bone marrow's ability to correct hyperglycemia in vivo has been shown in cases where animals with islets transplanted into bone marrow had a higher chance to reach euglycemia than islets transplanted in the liver [46]. With the hypothesis that bone marrow supporting human islet in vitro includes increase β cells, we used our established quantification method to evaluate effects of bone marrow on the quantity of human islet β cells during culture with or without bone marrow.…”

Section: Discussionmentioning

confidence: 99%

Bone marrow increases human islets insulin positive cells in co-culture: quantification with flow cytometry

Wang¹,

Xiong²,

Hassani³

et al. 2011

JDM

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We have previously demonstrated that allogeneic human bone marrow (BM) supports human islet function and longevity in vitro. We hypothesize that BM supporting human islets may include to increase β-cell in cultured islets. In this study, we developed a method to quantify insulin-producing β cells from cultured islets by using immunofluorescent staining and flow cytometry analysis to explore this possibility. The results show that human islets cocultured with BM for 39 days contained a significantly higher number of insulin-positive β cells (42.3% ± 4.5%) compared to the islet-only cultures (1.15% ± 0.78%), and increased insulin release levels evaluated by ELISA is consistent with increased β cells in same culture condition. Human islet culture with BM significantly increase β-cells while islet only culture lost β-cells in same culture period supports the possibility of BM increasing β-cells in cultured islets.

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Section: Discussionmentioning

confidence: 99%

Bone marrow increases human islets insulin positive cells in co-culture: quantification with flow cytometry

Wang¹,

Xiong²,

Hassani³

et al. 2011

JDM

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“…The isografts and allografts showed positive staining for insulin and glucagon and no evidence of allograft rejection up to 21 days post transplant, whereas the xenografts were acutely rejected [47]. The authors concluded that the bone marrow is capable of maintaining pancreatic islets in the absence of immunosuppression and, thus, can constitute an immunoprivileged environment for engraftment [47]. In another study from Italy [47], syngeneic islets engrafted efficiently in the bone marrow of streptozotocin-induced diabetic mice, as evident from glucose metabolism that was similar to that of nondiabetic mice [48•].…”

Section: The Bone Marrowmentioning

confidence: 94%

“…No immunosuppression was used. The isografts and allografts showed positive staining for insulin and glucagon and no evidence of allograft rejection up to 21 days post transplant, whereas the xenografts were acutely rejected [47]. The authors concluded that the bone marrow is capable of maintaining pancreatic islets in the absence of immunosuppression and, thus, can constitute an immunoprivileged environment for engraftment [47].…”

Section: The Bone Marrowmentioning

confidence: 99%

“…In fact, it is still used for blood transfusion in children. In an experimental study in rats, islet isografts, allografts, and xenografts (tilapia islets to rats) were transplanted into the bone marrow (tibia) of nondiabetic recipients [47]. No immunosuppression was used.…”

Section: The Bone Marrowmentioning

confidence: 99%

“…The authors concluded that the bone marrow is capable of maintaining pancreatic islets in the absence of immunosuppression and, thus, can constitute an immunoprivileged environment for engraftment [47]. In another study from Italy [47], syngeneic islets engrafted efficiently in the bone marrow of streptozotocin-induced diabetic mice, as evident from glucose metabolism that was similar to that of nondiabetic mice [48•]. Islets transplanted into the bone marrow had a higher probability to reach euglycemia than islets transplanted into the liver, and showed a compact morphology with a conserved ratio between α and β cells [48•].…”

Section: The Bone Marrowmentioning

confidence: 99%

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Islet Transplantation: Alternative Sites

Rajab

2010

Curr Diab Rep

103

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The portal vein is currently the site of choice for clinical islet transplantation, even though it is far from being an ideal site. Low oxygen tension and the induction of an inflammatory response impair islet implantation and lead to significant early loss. Even if enough islets survive the early implantation period to render insulin independence, few patients maintain it. Therefore, the search for an ideal site for islet transplantation continues. Experimentally, islets have been transplanted into the portal vein, kidney subcapsule, spleen, pancreas, peritoneum, omentum, gastrointestinal wall, testis, thymus, bone marrow, anterior chamber of the eye, cerebral ventricles, and subcutaneous and intramuscular spaces. Some of these sites are suitable for gathering scientific data, whereas others have potential clinical application. Varying degrees of success have been reported with the use of all these transplant sites in an experimental setting. However, the optimal transplant site remains to be finally established.

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