The bone marrow stem cell niche grows up: mesenchymal stem cells and macrophages move in

Ehninger, Armin; Trumpp, Andreas

doi:10.1084/jem.20110132

Cited by 496 publications

(402 citation statements)

References 56 publications

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“…The complete mechanism of G-CSF HSPC mobilisation is still unclear, but G-CSF mediated disruption of the CXCR4-CXCL12 axis appears to be an important step (Ehninger and Trumpp, 2011). Thus, the presence of G-CSF and VEGF simultaneously, as observed post-injury, may synergise to promote MSC mobilisation.…”

Section: Pharmacological Interventions To Enhance Tissue Repairmentioning

confidence: 99%

Pericytes, mesenchymal stem cells and their contributions to tissue repair

Wong

Rowley

Redpath

et al. 2015

Pharmacology & Therapeutics

152

110

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Regenerative medicine using mesenchymal stem cells for the purposes of tissue repair has garnered considerable public attention due to the potential of returning tissues and organs to a normal, healthy state after injury or damage has occurred. To achieve this, progenitor cells such as pericytes and bone marrow-derived mesenchymal stem cells can be delivered exogenously, mobilised and recruited from within the body or transplanted in the form organs and tissues grown in the laboratory from stem cells. In this review, we summarise the recent evidence supporting the use of endogenously mobilised stem cell populations to enhance tissue repair along with the use of mesenchymal stem cells and pericytes in the development of engineered tissues. Finally, we conclude with an overview of currently available therapeutic options to manipulate endogenous stem cells to promote tissue repair.

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Section: Pharmacological Interventions To Enhance Tissue Repairmentioning

confidence: 99%

Pericytes, mesenchymal stem cells and their contributions to tissue repair

Wong

Rowley

Redpath

et al. 2015

Pharmacology & Therapeutics

152

110

View full text Add to dashboard Cite

show abstract

“…However, osteoblasts do not act alone as niche cells. Therefore, a deeper understanding of HSC niches and the contribution of different niche cell types to different HSC functions is needed (Ehninger and Trumpp, 2011;Lander et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Impact of substrate elasticity on human hematopoietic stem and progenitor cell adhesion and motility

Lee-Thedieck

Rauch

Fiammengo

et al. 2012

Journal of Cell Science

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SummaryIn the bone marrow, hematopoietic stem cells (HSCs) reside in endosteal and vascular niches. The interactions with the niches are essential for the maintenance of HSC number and properties. Although the molecular nature of these interactions is well understood, little is known about the role of physical parameters such as matrix elasticity. Osteoblasts, the major cellular component of the endosteal HSC niche, flatten during HSC mobilization. We show that this process is accompanied by osteoblast stiffening, demonstrating that not only biochemical signals but also mechanical properties of the niche are modulated. HSCs react to stiffer substrates with increased cell adhesion and migration, which could facilitate the exit of HSCs from the niche. These results indicate that matrix elasticity is an important factor in regulating the retention of HSCs in the endosteal niche and should be considered in attempts to propagate HSCs in vitro for clinical applications.

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“…The bone marrow resident adult stem cells mainly include Hematopoietic Stem Cells (HSCs) and Mesenchymal Stem Cells (MSCs) or mesodermal stromal cells (Ehninger and Trumpp, 2011). Although, MSCs have now been isolated from various other tissues of mesodermal origin e.g., adipose tissue, muscle, bone, tendon, as well as tissues of non-mesodermal origin e.g., brain, spleen, liver, kidney, lung, pancreas, thymus etc.…”

Section: Introductionmentioning

confidence: 99%

A Comparative Analysis of Invasive and Non-Invasive Method of Bone Marrow Stromal Cell Isolation

Santra¹,

Gupta²,

Singh³

et al. 2015

Asian J. of Animal and Veterinary Advances

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The conventional method of Bone Marrow Stromal Cell (BMSC) isolation from live subjects are complex due to involvement of lot of expert personnel and pre and post operational medication and cares. Moreover, the concerning ethical issues also pose lots of restrictions for isolation of BMSC's making stem cells research restricted to certain elite laboratories only. This study aims to compare between the regular aspiration (invasive) method and an alternative, straight forward and non-invasive method of BMSC harvest. The BMSCs were harvested by both invasive and non-invasive methods and cultured in MSC (Mesenchymal Stem Cell) medium. The cells were undergone visual assessment of growth dynamics as well as identification and characterization of MSC cells, based on microscopic examination. Both of these tested methods successfully yielded significant amount of BMSC that were found to be identical in morphology, growth dynamics and in vitro cultural properties. Unlike the invasive method that requires a live animal, the non-invasive method relies on post-slaughtered bone and therefore obviates the requirement of skill personnel and setup. Eventually, the used bone from already dead animal no longer becomes the issue of conflict with animal ethics and welfare. The ease in cell harvest and lack of ethical barrier would definitely make BMSC harvest convenient and therefore, anticipated to be well accepted in resource poor laboratories around the globe.

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The bone marrow stem cell niche grows up: mesenchymal stem cells and macrophages move in

Abstract: Ehninger and Trumpp discuss the role of monocytes/macrophages and other niche cells in the regulation of HSC mobilization and retention.

Cited by 496 publications

References 56 publications

Pericytes, mesenchymal stem cells and their contributions to tissue repair

Pericytes, mesenchymal stem cells and their contributions to tissue repair

Impact of substrate elasticity on human hematopoietic stem and progenitor cell adhesion and motility

A Comparative Analysis of Invasive and Non-Invasive Method of Bone Marrow Stromal Cell Isolation

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