The C-terminal Cytosolic Region of Rim21 Senses Alterations in Plasma Membrane Lipid Composition

Nishino, Kanako; Obara, Keisuke; Kihara, Akio

doi:10.1074/jbc.m115.674382

Cited by 27 publications

(50 citation statements)

References 43 publications

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“…In that previous study, we showed that GFP-Rim21C was associated with the PM and the yeast bud neck, where lipid asymmetry is altered locally [13] in WT cells under normal conditions, whereas it dissociated from the PM upon activation of the Rim101 pathway (under alkali conditions or in cells with mutations in lipid translocases) [22] (see also Fig. 3D).…”

Section: Disruption Of Er-pm Contact Causes Constitutive Activation Omentioning

confidence: 85%

“…The amplified fragments were inserted directly into the chromosome by homologous recombination. Integration of P ADH -GFP-RIM21C to the yeast chromosome was performed as described previously [22]. Successful deletions of the genes and tagging were confirmed by genomic PCR and/or fluorescence microscopy.…”

Section: Genetic Manipulation and Plasmidsmentioning

confidence: 99%

“…We recently reported that the C-terminal cytosolic region of Rim21 (Rim21C) senses altered lipid asymmetry, probably through the recognition of altered lipid composition in the inner leaflet of the PM [22]. In that previous study, we showed that GFP-Rim21C was associated with the PM and the yeast bud neck, where lipid asymmetry is altered locally [13] in WT cells under normal conditions, whereas it dissociated from the PM upon activation of the Rim101 pathway (under alkali conditions or in cells with mutations in lipid translocases) [22] (see also Fig.…”

Section: Disruption Of Er-pm Contact Causes Constitutive Activation Omentioning

confidence: 99%

“…For example, external alkalization may induce lipid asymmetry changes. In the Rim101 pathway, the PM protein Rim21 acts as a sensor and may recognize altered lipid composition of the inner leaflet of the PM [21,22]. Upon activation of Rim21, downstream molecules, including the arrestin-related protein Rim8, endosomal sorting complex required for transport (ESCRT) proteins, the Bro1 family protein Rim20, and the calpain-like protein Rim13, are recruited to the PM and form a complex, followed by the activation of the transcription factor Rim101 by proteolytic processing [23][24][25].…”

Section: Introductionmentioning

confidence: 99%

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The Rim101 pathway contributes to ER stress adaptation through sensing the state of plasma membrane

Obara

Kihara

2016

Biochemical Journal

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Abbreviation list: DMSO, dimethyl sulfoxide; ER, endoplasmic reticulum; ESCRT, endosomal sorting complex required for transport; PM, plasma membrane; PtdSer, phosphatidylserine; Rim21C, C-terminal cytosolic region of Rim21; WT, wild-type 2 Summary statementThe sensor protein for plasma membrane lipid asymmetry, Rim21, detects ER stress by sensing alterations in the state of the plasma membrane, and triggers an adaptation response via the Rim101 pathway. 3 AbstractYeast cells sense alterations in the plasma membrane (PM) lipid asymmetry and external alkalization by the sensor protein Rim21, which functions in the Rim101 pathway. Rim101 signaling is initiated at the PM by the recruitment of the Rim101 signaling complex. The PM physically associates with the cortical endoplasmic reticulum (ER) to form ER-PM contact sites, where several signaling events, lipid exchange, and ion transport take place. In the present study, we investigated the spatial relationship between ER-PM contact sites and the sites of Rim101 signaling. Rim101 signaling mostly proceeds outside

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Section: Disruption Of Er-pm Contact Causes Constitutive Activation Omentioning

confidence: 85%

Section: Genetic Manipulation and Plasmidsmentioning

confidence: 99%

Section: Disruption Of Er-pm Contact Causes Constitutive Activation Omentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The Rim101 pathway contributes to ER stress adaptation through sensing the state of plasma membrane

Obara

Kihara

2016

Biochemical Journal

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“…This activation is dependent on Rim21, suggesting that plasma membrane depolarization is a key signal sensed by Rim21 (160). Moreover, a recent study suggests that alterations in lipid asymmetry cause changes in lipid composition and local charge on the inner leaflet, leading to dissociation of the Rim21 complex from the plasma membrane and recruitment of downstream proteins (161). Thus Rim21 senses external alkalization, as well as altered lipid asymmetry.…”

Section: Adapting To Individual Stressesmentioning

confidence: 99%

Stress Adaptation

et al. 2017

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Fungal species display an extraordinarily diverse range of lifestyles. Nevertheless, the survival of each species depends on its ability to sense and respond to changes in its natural environment. Environmental changes such as fluctuations in temperature, water balance or pH, or exposure to chemical insults such as reactive oxygen and nitrogen species exert stresses that perturb cellular homeostasis and cause molecular damage to the fungal cell. Consequently, fungi have evolved mechanisms to repair this damage, detoxify chemical insults, and restore cellular homeostasis. Most stresses are fundamental in nature, and consequently, there has been significant evolutionary conservation in the nature of the resultant responses across the fungal kingdom and beyond. For example, heat shock generally induces the synthesis of chaperones that promote protein refolding, antioxidants are generally synthesized in response to an oxidative stress, and osmolyte levels are generally increased following a hyperosmotic shock. In this article we summarize the current understanding of these and other stress responses as well as the signaling pathways that regulate them in the fungi. Model yeasts such as Saccharomyces cerevisiae are compared with filamentous fungi, as well as with pathogens of plants and humans. We also discuss current challenges associated with defining the dynamics of stress responses and with the elaboration of fungal stress adaptation under conditions that reflect natural environments in which fungal cells may be exposed to different types of stresses, either sequentially or simultaneously.

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Extracellular pH and high concentration of potassium regulate the primary necrosis in the yeast Saccharomyces cerevisiae

2021

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Extracellular pH has a signi cant impact on the physiology of the yeast cell, but its role in cell death has not been thoroughly investigated. We studied the effect of extracellular pH on the development of primary necrosis in Saccharomyces cerevisiae yeast under two general conditions leading to cell death. The rst is sugar induced cell death (SICD), and the second is death caused by several speci c gene deletions, which have been recently identi ed in a systematic screen. It was shown that in both cases, primary necrosis is suppressed at neutral pH. SICD was also inhibited by the protonophore dinitrophenol (DNP) and 150 mM extracellular K+, with the latter condition also bene ting survival of cell dying due to gene mutations. Thus, we show that neutral pH can suppress different types of primary necrosis. We suggest that changes to the cellular membrane potential can play a central role in yeast cell death.

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The C-terminal Cytosolic Region of Rim21 Senses Alterations in Plasma Membrane Lipid Composition

Cited by 27 publications

References 43 publications

The Rim101 pathway contributes to ER stress adaptation through sensing the state of plasma membrane

The Rim101 pathway contributes to ER stress adaptation through sensing the state of plasma membrane

Stress Adaptation

Extracellular pH and high concentration of potassium regulate the primary necrosis in the yeast Saccharomyces cerevisiae

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