The C-terminal mutation beyond the catalytic site of brown spider phospholipase D significantly impacts its biological activities

Cunha, Laís Cardoso; Barreto, Lucas Passos; Valadares, Veronica S.; Oliveira, Camila Franco Batista; Vuitika, Larissa; Vilela, Maura Páscoa; Cino, Elio A.; Silva, Adolfo Henrique de Moraes; Nagem, R.A.P.; Chávez-Olórtegui, Carlos; Dias-Lopes, Camila; Molina, Franck; Felicori, Liza

doi:10.1016/j.biochi.2023.03.010

Cited by 2 publications

(2 citation statements)

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“…[ 32 ] Many studies have confirmed that C‐terminal mutation is beneficial to improve the catalytic activity and thermostability of the enzyme. [ 33,34 ] Xu et al. engineered the C‐terminal region to improve the thermostability of nitrilase.…”

Section: Introductionmentioning

confidence: 99%

“…[31] Li et al enhanced the thermostability of Aspergillus niger xylanase by saturation mutating the highly flexible region, resulting in a 137.6fold increase in the half-life of the mutant at 50 • C. [32] Many studies have confirmed that C-terminal mutation is beneficial to improve the catalytic activity and thermostability of the enzyme. [33,34] Xu et al engineered the C-terminal region to improve the thermostability of nitrilase. [35] In general, saturation mutagenesis and C-terminal modification are effective method to improve enzyme properties.…”

Section: Introductionmentioning

confidence: 99%

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Enhancing the thermostability and catalytic activity of Bacillus subtilis chitosanase by saturation mutagenesis of Lys242

Guo,

Gao,

Zhang

et al. 2023

Biotechnology Journal

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Catalysis activity and thermostability are some of the fundamental characteristic of enzymes, which are of great significance to their industrial applications. Bacillus subtilis chitosanase BsCsn46A is a kind of enzyme with good catalytic activity and stability, which can hydrolyze chitosan to produce chitobiose and chitotriose. In order to further improve the catalytic activity and stability of BsCsn46A, saturation mutagenesis of the C‐terminal K242 of BsCsn46A was performed. The results showed that the six mutants (K242A, K242D, K242E, K242F, K242P and K242T) showed increased catalytic activity on chitosan. The catalytic activity of K242P increased from 12971 ± 597 U/mg of wild type to 17820 ± 344 U/mg, and the thermostability of K242P increased by 2.27%. In order to elucidate the reason for the change of enzymatic properties, hydrogen network, molecular docking and molecular dynamics simulation were carried out. The hydrogen network results showed that all the mutants lose their interaction with Asp6 at 242 site, thereby increasing the flexibility of Glu19 at the junction sites of α1 and loop1. Molecular dynamics results showed that the RMSD of K242P was lower at both 313 and 323 K than that of other mutants, which supported that K242P had better thermostability. The catalytic activity of mutant K242P reached 17820.27 U/mg, the highest level reported so far, which could be a robust candidate for the industrial application of chitooligosaccharide (COS) production.This article is protected by copyright. All rights reserved

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Section: Introductionmentioning

confidence: 99%