The calcium-permeable channel OSCA1.3 regulates plant stomatal immunity

Thor, Kathrin; Jiang, Shushu; Michard, Erwan; George, Jeoffrey; Scherzer, Sönke; Huang, Shouguang; Dindas, Julian; Derbyshire, Paul; Leitão, Nuno; DeFalco, Thomas A.; Köster, Philipp; Hunter, Kerri; Kimura, Shigenobu; Gronnier, Julien; Stransfeld, Lena; Kadota, Yasuhiro; Bücherl, Christoph A.; Charpentier, Myriam; Wrzaczek, Michael; MacLean, Daniel; Oldroyd, Giles; Menke, Frank L. H.; Roelfsema, M. Rob G.; Hedrich, Rainer; Feijó, José A.; Zipfel, Cyril

doi:10.1038/s41586-020-2702-1

Cited by 248 publications

(184 citation statements)

References 62 publications

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“…To understand how PLL4 and 5 are themselves regulated, we interrogated public databases (Heazlewood et al, 2008; Mergner et al, 2020) for phosphosites within these proteins. Several clustered, conserved sites were identified in the N-terminus of PLL4 and 5 that conformed to a previously identified [S/T]-X-X-L motif (Figure 1D, S2), which is targeted by BIK1 and PBL1 in other substrates (Kadota et al, 2014; Thor et al, 2020). To test whether BIK1 directly phosphorylates PLL4, we expressed various fragments of PLL4 as MBP fusions and subjected them to in vitro kinase assays.…”

Section: Resultssupporting

confidence: 83%

A conserved regulatory module regulates receptor kinase signaling in immunity and development

DeFalco

Anne

James

et al. 2021

Preprint

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Ligand recognition by cell-surface receptors underlies development and immunity in both animals and plants. Modulating receptor signaling is critical for appropriate cellular responses but the mechanisms ensuring this are poorly understood. Here, we show that signaling by plant receptors for pathogen-associated molecular patterns (PAMPs) in immunity and CLAVATA3/EMBRYO SURROUNDING REGION-RELATED peptides (CLEp) in development employ a similar regulatory module. In the absence of ligand, signaling is dampened through association with specific type-2C protein phosphatases (PP2Cs). Upon activation, PAMP and CLEp receptors phosphorylate divergent cytosolic kinases, which, in turn, phosphorylate the phosphatases, thereby promoting their release from the receptor complexes. Our work reveals a regulatory circuit shared between immune and developmental receptor signaling, which may have broader important implications for plant receptor kinase-mediated signaling in general.

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Section: Resultssupporting

confidence: 83%

A conserved regulatory module regulates receptor kinase signaling in immunity and development

DeFalco

Anne

James

et al. 2021

Preprint

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“…In Arabidopsis , five LysM RLKs, and three LysM RLPs have been identified (Shinya et al, 2015). Furthermore, lectin domains often perceive ligands composed of glycans and carbohydrates and can be classified into three subfamilies, including L‐type, C‐type, and G‐type lectin domain (Vaid et al, 2013; Lannoo and Van Damme, 2014; Jamieson et al, 2018). In Arabidopsis , the L‐type lectin comprises of 45 RLKs and six RLPs, while the G‐type lectin comprises of 39 RLKs and three RLPs, and only one RLK C‐type lectin has been predicted (Lannoo and Van Damme, 2014; Bellande et al, 2017; Jamieson et al, 2018).…”

Section: General Features Of Rlks and Rlpsmentioning

confidence: 99%

“…Recent studies have provided new insights into calcium signaling. For instance, upon MAMP perception, the Ca 2+ channel, OSCA1.3, regulates stomatal closure, and subsequently, it is regulated by BIK1, leading to an increase of Ca 2+ influx (Thor et al, 2020). In addition, the CYCLIC NUCLEOTIDE-GATED CHANNEL 2 (CNGC2) and CNGC4 were reported to be essential in the formation of a functional calcium channel, that after MAMP perception, is phosphorylated by BIK1, resulting in an increase of calcium in the cytoplasm (Tian et al, 2019).…”

Section: Intracellular Signaling Upon Activation Of Rlk Rlp and Rlcmentioning

confidence: 99%

Plant plasma membrane‐resident receptors: Surveillance for infections and coordination for growth and development

Manhães

Ortiz-Morea

et al. 2021

JIPB

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As sessile organisms, plants are exposed to pathogen invasions and environmental fluctuations. To overcome the challenges of their surroundings, plants acquire the potential to sense endogenous and exogenous cues, resulting in their adaptability. Hence, plants have evolved a large collection of plasma membrane-resident receptors, including RECEPTOR-LIKE KINASEs (RLKs) and RECEPTOR-LIKE PROTEINs (RLPs) to perceive those signals and regulate plant growth, development, and immunity. The ability of RLKs and RLPs to recognize distinct ligands relies on diverse categories of extracellular domains evolved. Co-regulatory receptors are often required to associate with RLKs and RLPs to facilitate cellular signal transduction. RECEPTOR-LIKE CYTOPLASMIC KINASEs (RLCKs) also associate with the complex, bifurcating the signal to key signaling hubs, such as MITOGEN-ACTIVATED PROTEIN KINASE (MAPK) cascades, to regulate diverse biological processes. Here, we discuss recent knowledge advances in understanding the roles of RLKs and RLPs in plant growth, development, and immunity, and their connection with co-regulatory receptors, leading to activation of diverse intracellular signaling pathways.

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“…Although we still have not pinpointed the genetic identity of Ca 2+ transport systems in plants, the occurrence of cytosolic Ca 2+ variations induced by environmental stimuli is well established (Jezek & Blatt, 2017; Konrad et al ., 2018). Recently, a family of channels, the OSCA, was found to be PM ion channels involved in Ca 2+ signalling (Yuan et al ., 2014; Thor et al ., 2020). Interestingly, the OSCA channels are permeable to Ca 2+ but also to a similar extent to Na + (Thor et al ., 2020).…”

Section: Ion Flux Coordination Under the Control Of Ionic Conditions mentioning

confidence: 99%

“…Recently, a family of channels, the OSCA, was found to be PM ion channels involved in Ca 2+ signalling (Yuan et al ., 2014; Thor et al ., 2020). Interestingly, the OSCA channels are permeable to Ca 2+ but also to a similar extent to Na + (Thor et al ., 2020). The VM harbours different ion transport systems that are sensitive to cytosolic Ca 2+ .…”

Section: Ion Flux Coordination Under the Control Of Ionic Conditions mentioning

confidence: 99%

Connecting vacuolar and plasma membrane transport networks

Cubero-Font

Angeli

2020

New Phytologist

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The coordinated control of ion transport across the two major membranes of differentiated plant cells, the plasma and the vacuolar membranes, is fundamental in cell physiology. The stomata responses to the fluctuating environmental conditions are an illustrative example. Indeed, they rely on the coordination of ion fluxes between the different cell compartments. The cytosolic environment, which is an interface between intracellular compartments, and the activity of the ion transporters localised in the different membranes influence one each other. Here we analyse the molecular mechanisms connecting and modulating the transport processes at both the plasma and the vacuolar membranes of guard cells.

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The calcium-permeable channel OSCA1.3 regulates plant stomatal immunity

Cited by 248 publications

References 62 publications

A conserved regulatory module regulates receptor kinase signaling in immunity and development

A conserved regulatory module regulates receptor kinase signaling in immunity and development

Plant plasma membrane‐resident receptors: Surveillance for infections and coordination for growth and development

Connecting vacuolar and plasma membrane transport networks

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