The cancer cell secretome: A good source for discovering biomarkers?

Pavlou, Maria; Diamandis, Eleftherios P.

doi:10.1016/j.jprot.2010.04.003

Cited by 184 publications

(149 citation statements)

References 76 publications

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“…In many diseases, secreted proteins create conditions that are favorable to the disorder, such as those that promote cancer metastasis (23). Therefore, knowledge of the qualitative and quantitative composition of the cell IF may be crucial to understand the biology of cell interaction.…”

Section: Discussionmentioning

confidence: 99%

Two-dimensional gel electrophoresis analysis of the leiomyoma interstitial fluid reveals altered protein expression with a possible involvement in pathogenesis

et al. 2015

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Abstract. Uterine leiomyoma is the most common smooth benign neoplasm. In the present study, we analyzed the global interstitial fluid (IF) profile of leiomyoma vs. normal myometrium to identify protein dysregulation involved in leiomyoma pathogenesis. Two-dimensional gel electrophoresis and mass spectrometry were used to generate and compare the global interstitial fluid profiles of the leiomyoma and of the normal tissue. Two proteins were validated by immunohistochemistry. By comparing the interstitial fluid profile of the leiomyoma with that of the normal myometrium, the levels of seven proteins were found to be significantly different: four structural organization proteins (desmin, prelamin-A/C, transgelin and α-actinin-1), an inflammatory response (α1-antitrypsin), a response to oxidative stress (peroxiredoxin-2), and a folding protein (heat shock 70 kDa protein 1A/1B). Desmin, α1-antitrypsin and peroxiredoxin-2 were upregulated in the leiomyoma, whereas heat shock 70 kDa protein 1A/1B, α-actinin-1, prelamin-A/C and transgelin were downregulated. Desmin and α1-antitrypsin were further validated by immunohistochemistry. By identifying proteins with altered expression levels compared to the myometrium from several pathways of the leiomyoma pathogenesis, we found the leiomyoma interstitial fluid to have a characteristic proteomic profile. A better appreciation of the pathophysiology of the disease can be useful in the development of conservative treatments that serve as viable alternatives to hysterectomy.

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Section: Discussionmentioning

confidence: 99%

Two-dimensional gel electrophoresis analysis of the leiomyoma interstitial fluid reveals altered protein expression with a possible involvement in pathogenesis

et al. 2015

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“…Cancer secretome studies can increase analysis specificity while minimizing the number of biomarkers tested, particularly with respect to high-abundance proteins found in plasma [21]. One approach for biomarker discovery is the analysis of CM from HCC cell lines [10].…”

Section: Discussionmentioning

confidence: 99%

Secretory/releasing proteome-based identification of plasma biomarkers in HBV-associated hepatocellular carcinoma

Yang

Rong

Xiao

et al. 2013

Sci. China Life Sci.

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For successful therapy, hepatocellular carcinoma (HCC) must be detected at an early stage. Herein, we used a proteomic approach to analyze the secretory/releasing proteome of HCC tissues to identify plasma biomarkers. Serum-free conditioned media (CM) were collected from primary cultures of cancerous tissues and surrounding noncancerous tissues. Proteomic analysis of the CM proteins permitted the identification of 1365 proteins. The enriched molecular functions and biological processes of the CM proteins, such as hydrolase activity and catabolic processes, were consistent with the liver being the most important metabolic organ. Moreover, 19% of the proteins were characterized as extracellular or membrane-bound. For validation, secretory proteins involved in transforming growth factor-β signaling pathways were validated in plasma samples. Alphafetoprotein (AFP), metalloproteinase (MMP)1, osteopontin (OPN), and pregnancy-specific beta-1-glycoprotein (PSG)9 were significantly increased in HCC patients. The overall performance of MMP1 and OPN in the diagnosis of HCC remained greater than that of AFP. In addition, this study represents the first report of MMP1 as a biomarker with a higher sensitivity and specificity than AFP. Thus, this study provides a valuable resource of the HCC secretome with the potential to investigate serological biomarkers. MMP1 and OPN could be used as novel biomarkers for the early detection of HCC and to improve the sensitivity of biomarkers compared with AFP. hepatocellular carcinoma (HCC), secretome, biomarker, MMP1, OPN, PSG9 Citation:Yang L, Rong W Q, Xiao T, et al. Secretory/releasing proteome-based identification of plasma biomarkers in HBV-associated hepatocellular carcinoma. Sci China Life Sci, 2013Sci, , 56: 638-646, doi: 10.1007 Approximately 600000 new cases of hepatocellular carcinoma (HCC) are diagnosed each year, of which approximately 55% are detected in China [1]. The relationship between HCC and persistent hepatitis B virus (HBV) infection has been well documented. More than 20 million individuals suffer from chronic HBV infection in China [2]. Surgical resection is the most effective treatment for HCC patients, but the reported five-year disease-free survival rate is only

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“…The identification of cancer cell secretome has uncovered mechanisms related to angiogenesis, invasion and metastasis, and can contribute to the development of new strategies for the efficient control of cancer. Moreover, secreted or shed proteins can reach body fluids including serum, an ideal sample source for early diagnosis and monitoring of cancer [1][2][3].…”

Section: Introductionmentioning

confidence: 99%

“…Novel lung cancer serum biomarkers are needed, because those identified to date, such as cancer embryonic antigen and squamous cell carcinoma (SCC) antigen, lack sufficient specificity and sensitivity to serve as an efficient biomarker [1].…”

Section: Introductionmentioning

confidence: 99%

“…To overcome this drawback, proteomic identification of conditioned media of cancer cell lines has recently attracted much attention [1][2][3]. Generally, the identified proteins were already known to be involved in cancer development and progression, and subsequent studies validated the presence of several of them in sera from cancer patients.…”

Section: Introductionmentioning

confidence: 99%

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Secretomic analysis of large cell lung cancer cell lines using two-dimensional gel electrophoresis coupled to mass spectrometry

Yousefi¹,

Sarvari²,

Nakamura³

et al. 2012

Folia Histochem Cytobiol.

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Abstract:The secretome of cancer cells is a valuable source of biomarkers that can ultimately reach the serum or other body fluids. Cancer biomarkers can facilitate early diagnosis and monitoring of the disease, contribute to our understanding of tumor biology, and support the development of more efficient therapies. Recently, high-throughput proteomic analysis of the conditioned media of cancer cell lines has shown potential to identify novel biomarkers in cancer. We used two-dimensional gel electrophoresis coupled to liquid chromatography tandem mass spectrometry to identify the secretome of the large cell lung cancer cell lines QU-DB and Mehr-80, which were established from a Canadian and a Persian patient, respectively. A total of 130 distinct protein species were identified. Most of them were previously found in serum or other body fluids, the membrane compartment or conditioned media of other cancer cell lines. Some of the proteins that we identified, e.g. IL-6, triosephosphate isomerase, PGP9.5, a-enolase, Dickkopf-1, and peroxiredoxin-1 have been already known as putative serum markers for lung cancer, whereas others might be candidate markers for further validation in lung cancer body fluids such as IL-25, stathmin, vimentin, peptidyl-prolyl cis-trans isomerase A, transgelin-2, and chloride intracellular channel protein 4.

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The cancer cell secretome: A good source for discovering biomarkers?

Cited by 184 publications

References 76 publications

Two-dimensional gel electrophoresis analysis of the leiomyoma interstitial fluid reveals altered protein expression with a possible involvement in pathogenesis

Two-dimensional gel electrophoresis analysis of the leiomyoma interstitial fluid reveals altered protein expression with a possible involvement in pathogenesis

Secretory/releasing proteome-based identification of plasma biomarkers in HBV-associated hepatocellular carcinoma

Secretomic analysis of large cell lung cancer cell lines using two-dimensional gel electrophoresis coupled to mass spectrometry

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