The Capacity of Liver Microsomes to Form Benzo[a]Pyrene-Diolepoxide-DNA Adducts and Induction of Cytochrome P450 1A in Feral Fish Exposed to Pulp Mill Effluents

Kantoniemi, Asta; Vähäkangas, Kirsi; Oikari, Aimo

doi:10.1006/eesa.1996.0092

Cited by 20 publications

(4 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The expression levels of the Cyp1a protein and its mRNA and 7-ethoxyresorufin-O-deethylase (EROD) activity increase through AhR activation and therefore have been used for in vivo detection of the adverse impact of water contaminated with polycyclic aromatic hydrocarbons (PAHs) [17][18][19]. The present study used BNF, a typical PAH and AhR agonist, which has approximately the same potency as 2,3,7,8-tetrachlorodibenzop-dioxin in activating an in vitro AhR response [11,20].…”

Section: Cyp1a Gene Transcriptionmentioning

confidence: 99%

The feasibility of using mosquitofish (Gambusia affinis) for detecting endocrine‐disrupting chemicals in the freshwater environment

Kamata

Itoh

Nakajima

et al. 2011

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

We evaluated the utility of gene-transcriptional responses in the liver of mosquitofish (Gambusia affinis), a species introduced to many countries and therefore widely available, for detecting endocrine-disrupting activity in water. Exposure to β-naphthoflavone, an aryl hydrocarbon receptor (AhR) agonist, significantly increased the transcript of the cytochrome P4501A gene (cyp1a), peaking at 24 h, in both sexes at concentrations of 10 µg/L or more. 17β-Estradiol (E(2) ) at 500 ng/L increased the number of males showing gene transcription of precursors of yolk protein, vitellogenin (Vtga, Vtgb, and Vtgc), at 24, 48, and 72 h. Exposure for 48 h to bisphenol A (BPA), an estrogen mimic, also increased vtg-positive males at 1 mg/L or more. Leachate from a Japanese stable-type landfill significantly increased vtg-positive males after 48 h exposure, and the in vitro activity of the leachate against the estrogen receptor (ER) was estimated as an E(2) equivalent of 240 ng/L by yeast transfected with the ER. Chemical analysis showed that major contributors to the ER activation were BPA and 4-tert-octylphenol. This leachate and drainage water from a control-type landfill had AhR activities, estimated by yeast with the AhR, but had no significant effect on cyp1a transcription. These results showed that mosquitofish are suitable for detecting in vivo AhR and ER effects, but are insensitive to E(2).

show abstract

Section: Cyp1a Gene Transcriptionmentioning

confidence: 99%

The feasibility of using mosquitofish (Gambusia affinis) for detecting endocrine‐disrupting chemicals in the freshwater environment

Kamata

Itoh

Nakajima

et al. 2011

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

show abstract

“…Quantifying CYP1A activity through the ethoxyresorufin‐ O ‐deethylase (EROD) assay is a widely used technique to indirectly measure the amount of expressed enzyme , which measures the rate of resorufin production (a fluorescent compound). Furthermore, induction of the CYP1A system has been correlated to negative effects in vertebrates such as DNA adducts , cancer , and reproductive disorders .…”

Section: Introductionmentioning

confidence: 99%

Bioaccumulation of organochlorine contaminants and ethoxyresorufin‐o‐deethylase activity in southern California round stingrays (Urobatis halleri) exposed to planar aromatic compounds

Lyons

Lavado

Schlenk

et al. 2014

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

While contaminant concentrations have been reported for elasmobranchs around the world, no studies have examined bioaccumulation patterns across male and female age classes. The round stingray (Urobatis halleri) is a local benthic species that forages near areas of high organochlorine contamination and represents a good elasmobranch model. Polychlorinated biphenyls (PCBs), DDT, and chlordanes were measured in juvenile and adult male and female stingrays from areas in southern California, USA (n = 208), and a nearby offshore island, Santa Catalina (n = 34). Both mainland juvenile male and female stingrays showed a significant dilution effect. After maturity, summed contaminant concentrations significantly increased with size for adult males (median 11.1 µg/g lipid wt) and females (5.2 µg/g lipid wt). However, the rate of bioaccumulation was substantially greater in male stingrays than in females, likely a result of the females' ability to offload contaminants to offspring during pregnancy. In addition, males and females showed significant differences in their contaminant profiles, suggesting differential habitat use. Male and female stingrays collected from Santa Catalina Island had significantly lower concentrations (0.51 µg/g and 0.66 µg/g lipid wt, respectively), approximately 5 times less than those of mainland animals. Potential toxicity effects mediated through activation of the aryl hydrocarbon receptor were explored through ethoxyresorufin-O-deethylase (EROD) activity assays. Mainland male stingrays exhibited significantly greater EROD activities than Catalina males (481 pmol/min/mg protein and 55 pmol/min/mg protein, respectively); however, activity levels in female stingrays from both locations were comparable (297 pmol/min/mg protein and 234 pmol/min/mg protein, respectively) and lower than those in mainland males. The results suggest that PCBs and/or other structurally related contaminants may be inducing a biological response in mainland males but not females, possibly the result of a dampening effect of estradiol; however, the exact physiological repercussions of exposure remain to be determined.

show abstract

“…2002 poluidos podem estar expostos a xenobióticos os quais sofrem detoxificação mediada pela glutationa na sua forma reduzida, catalisada pela enzima glutationa Stransferase. Esta enzima de biotransformação tem sido estudada em trabalhos de campo no monitoramento de poluentes de origem industrial (Fenet et al 1998;Kantoniemi et al, 1996) e agrícola (Cho et al, 1999). Outros autores também relataram a detoxificação de outros compostos com propriedades fungicidas realizada pela GST.…”

Section: Resultsunclassified

Alterações bioquímicas e acúmulo em pacus (Metynnis argenteus) expostos ao paclobutrazol

Jonsson

Ferracini

Paraíba

et al. 2002

Sci. agric. (Piracicaba, Braz.)

View full text Add to dashboard Cite

O paclobutrazol, um regulador de crescimento vegetal com propriedades fungicidas, é muito utilizado na região Nordeste do Brasil em áreas de produção de frutíferas. Este composto pode permanecer estável por vários meses após sua aplicação no solo, sendo sua meia vida na água superior a três semanas. Corpos de água superficiais nas proximidades das culturas são portanto susceptíveis de serem contaminados pelo paclobutrazol, com o risco de promover efeitos adversos para a fauna aquática e para a saúde humana. No presente trabalho foi estudada a bioconcentração e algumas alterações bioquímicas, medidas pela atividade das enzimas transaminase glutâmico-oxalacética (TGO), lactato desidrogenase (LDH) e glutationa S-transferase (GST) em tecidos do peixe pacú prata (Metynnis argenteus), exposto ao paclobutrazol durante 28 dias. O paclobutrazol aumentou a atividade de LDH e GST no fígado para as concentrações de 3 mg L-1 (28º dia de exposição) e 30 mg L-1 (7º dia de exposição), respectivamente. No 28º dia, a concentração de paclobutrazol no músculo atingiu níveis residuais de 166 mg kg-1 para a concentração média determinada na água de 2,7 mg L-1, estimando-se um fator de bioconcentração (FBC) para a situação de equilíbrio equivalente a 73. A medida da atividade de LDH e GST pode ser utilizada como biomarcador de exposição ao paclobutrazol. Esta exposição deve estar em situações extremas para proporcionar risco significativo em humanos que se alimentam de peixes contendo resíduos do agroquímico.

show abstract

The Capacity of Liver Microsomes to Form Benzo[a]Pyrene-Diolepoxide-DNA Adducts and Induction of Cytochrome P450 1A in Feral Fish Exposed to Pulp Mill Effluents

Cited by 20 publications

References 16 publications

The feasibility of using mosquitofish (Gambusia affinis) for detecting endocrine‐disrupting chemicals in the freshwater environment

The feasibility of using mosquitofish (Gambusia affinis) for detecting endocrine‐disrupting chemicals in the freshwater environment

Bioaccumulation of organochlorine contaminants and ethoxyresorufin‐o‐deethylase activity in southern California round stingrays (Urobatis halleri) exposed to planar aromatic compounds

Alterações bioquímicas e acúmulo em pacus (Metynnis argenteus) expostos ao paclobutrazol

Contact Info

Product

Resources

About