The carbohydrate-binding module (CBM)-like sequence is crucial for rice CWA1/BC1 function in proper assembly of secondary cell wall materials

Sato, Kanna; Ito, Sachiko; Fujii, Takeo; Suzuki, Ryu; Takenouchi, Sachi; Nakaba, Satoshi; Funada, Ryo; Sano, Yuichi; Kajita, Shinya; Kitano, Hidemi; Katayama, Yoshihiro

doi:10.4161/psb.5.11.13342

Cited by 22 publications

(19 citation statements)

References 24 publications

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“…The COBRA sequence harbors a putative cellulose binding motif (CBM) (22), and the rice COBRA CBM was shown to bind cellulose (21). COBRA was tested against a variety of cell wall polymers, and was found to bind specifically to cellulose.…”

mentioning

confidence: 99%

The Arabidopsis COBRA Protein Facilitates Cellulose Crystallization at the Plasma Membrane

Sorek

Kijac

et al. 2014

Journal of Biological Chemistry

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Background:The COBRA gene is highly coexpressed with cellulose synthase genes, but its function remains unclear. Results: COBRA localizes at the plasma membrane and binds glucan chains. NMR studies indicate structural defects in cellulose in the mutant despite normal polymerization rate. Conclusion: COBRA functions downstream of cellulose biosynthesis. Significance: This work suggests that alignment of glucan chains into cellulose fibrils is facilitated by one or more proteins.

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confidence: 99%

The Arabidopsis COBRA Protein Facilitates Cellulose Crystallization at the Plasma Membrane

Sorek

Kijac

et al. 2014

Journal of Biological Chemistry

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“…An in vitro binding assay and in vivo rescue analysis revealed that Y46 and W72 are critical residues for binding with cellulose and targeting to cell walls. The importance of this domain and these residues was highlighted in the previous work [52]. Moreover, the mutated residues in Arabidopsis cob-1 to cob-3 are located in this domain and an aromatic residue (W55) is mutated in cob-3 [30].…”

Section: Discussionmentioning

confidence: 90%

Brittle Culm1, a COBRA-Like Protein, Functions in Cellulose Assembly through Binding Cellulose Microfibrils

et al. 2013

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Cellulose represents the most abundant biopolymer in nature and has great economic importance. Cellulose chains pack laterally into crystalline forms, stacking into a complicated crystallographic structure. However, the mechanism of cellulose crystallization is poorly understood. Here, via functional characterization, we report that Brittle Culm1 (BC1), a COBRA-like protein in rice, modifies cellulose crystallinity. BC1 was demonstrated to be a glycosylphosphatidylinositol (GPI) anchored protein and can be released into cell walls by removal of the GPI anchor. BC1 possesses a carbohydrate-binding module (CBM) at its N-terminus. In vitro binding assays showed that this CBM interacts specifically with crystalline cellulose, and several aromatic residues in this domain are essential for binding. It was further demonstrated that cell wall-localized BC1 via the CBM and GPI anchor is one functional form of BC1. X-ray diffraction (XRD) assays revealed that mutations in BC1 and knockdown of BC1 expression decrease the crystallite width of cellulose; overexpression of BC1 and the CBM-mutated BC1s caused varied crystallinity with results that were consistent with the in vitro binding assay. Moreover, interaction between the CBM and cellulose microfibrils was largely repressed when the cell wall residues were pre-stained with two cellulose dyes. Treating wild-type and bc1 seedlings with the dyes resulted in insensitive root growth responses in bc1 plants. Combined with the evidence that BC1 and three secondary wall cellulose synthases (CESAs) function in different steps of cellulose production as revealed by genetic analysis, we conclude that BC1 modulates cellulose assembly by interacting with cellulose and affecting microfibril crystallinity.

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“…In addition, the encoded proteins and functions of the 20 genes were annotated, and many were associated with fiber development (Table 4). Gh_ D11G1626 encoded a COBRA-like protein 4, and expressed in the fiber SCW biosynthesis stages; the function of COBRA-like protein has been reported in sorghum and rice, which were involved in SCW cellulose biosynthesis (Dai et al 2011;Li et al 2019;Sato et al 2010), so Gh_D11G1626 is probably involved in the SWC biosynthesis in cotton. Gh_D13G1900 and Gh_ A01G1474 encoded WAT1-related proteins, and showed specifically high expression level at the fiber SCW biosynthesis stages; the WAT1-related protein was probably related to high fiber yield in cotton (Liu et al 2018a).…”

Section: Candidate Genes Are Probably Involved In the Development Of mentioning

confidence: 99%

Identification of candidate genes controlling fiber quality traits in upland cotton through integration of meta-QTL, significant SNP and transcriptomic data

et al. 2020

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Background Meta-analysis of quantitative trait locus (QTL) is a computational technique to identify consensus QTL and refine QTL positions on the consensus map from multiple mapping studies. The combination of meta-QTL intervals, significant SNPs and transcriptome analysis has been widely used to identify candidate genes in various plants. Results In our study, 884 QTLs associated with cotton fiber quality traits from 12 studies were used for meta-QTL analysis based on reference genome TM-1, as a result, 74 meta-QTLs were identified, including 19 meta-QTLs for fiber length; 18 meta-QTLs for fiber strength; 11 meta-QTLs for fiber uniformity; 11 meta-QTLs for fiber elongation; and 15 meta-QTLs for micronaire. Combined with 8 589 significant single nucleotide polymorphisms associated with fiber quality traits collected from 15 studies, 297 candidate genes were identified in the meta-QTL intervals, 20 of which showed high expression levels specifically in the developing fibers. According to the function annotations, some of the 20 key candidate genes are associated with the fiber development. Conclusions This study provides not only stable QTLs used for marker-assisted selection, but also candidate genes to uncover the molecular mechanisms for cotton fiber development.

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The carbohydrate-binding module (CBM)-like sequence is crucial for rice CWA1/BC1 function in proper assembly of secondary cell wall materials

Cited by 22 publications

References 24 publications

The Arabidopsis COBRA Protein Facilitates Cellulose Crystallization at the Plasma Membrane

The Arabidopsis COBRA Protein Facilitates Cellulose Crystallization at the Plasma Membrane

Brittle Culm1, a COBRA-Like Protein, Functions in Cellulose Assembly through Binding Cellulose Microfibrils

Identification of candidate genes controlling fiber quality traits in upland cotton through integration of meta-QTL, significant SNP and transcriptomic data

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