The carbon source-dependent pattern of antimicrobial activity and gene expression in Pseudomonas donghuensis P482

Matuszewska, Marta; Maciag, Tomasz; Rajewska, Magdalena; Wierzbicka, Aldona; Jafra, Sylwia

doi:10.1038/s41598-021-90488-w

Cited by 20 publications

(46 citation statements)

References 131 publications

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“…In all three cases, the expression of the investigated genes in the presence of both types of exudates did not significantly differ (log2FC <1.5) from the expression in 1C medium alone (Dataset S1). However, in our recent study (44) we have found that the expression of BV82_1009 and BV82_4709 can be detected in unsupplemented 1C medium, here used as the reference (background), therefore the results from the RNAseq experiment can only be interpreted in terms the influence of exudates on the existing expression status of these genes, and they do not provide the information wheather the basal expression is high or low.…”

Section: Resultsmentioning

confidence: 94%

“…However, there were also two other genes linked to this family of efflux transporters which were upregulated in response to tomato. What is interesting about this family of efflux pumps is that, in P. donghuensis P482, inactivation of gene BV82_4243 encoding another RND-type transporter resulted in a reduced antibacterial activity of the mutant strain against Dickeya solani , as well as in the lack of export of the fluorescent siderophore pyoverdine (44). This implies that proteins from the family of RND-type transporters may be involved not only in defence against potentially harmful chemicals, presumably involving secondary metabolities of plants, but also in the export of the strain’s own metabolites.…”

Section: Discussionmentioning

confidence: 99%

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Shared and host-specific transcriptomic response of Pseudomonas donghuensis P482 to the exudates of tomato (Dicot) and maize (Monocot) shed light on the host-adaptive traits in the promiscuous root colonizing bacteria

Krzyżanowska¹,

Jablonska²,

Kaczyński

et al. 2022

Preprint

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Plants of different genotypes and physiological states recruit different populations of root microbiota. The selection is driven by the immune response of the plant and the composition of root exudates. Some bacteria, including Pseudomonas spp., are promiscuous root colonizers. It is yet unclear what particular changes in lifestyle enable them to thrive in the company of different plant hosts. In this study, we used RNAseq to identify genes of the differential (host-specific) and shared (host-independent) transcriptomic responses of a biocontrol strain Pseudomonas donghuensis P482 to the root exudates of two phylogenetically distinct plant species, tomato (Dicot) and maize (Monocot), both of which can be colonized by the bacterium. The host-independent response of P482 to exudates involved upregulated expression of arsenic resistance genes and bacterioferritin synthesis. Contrary, we observed downregulation of pathways related to sulfur assimilation, sensing of ferric citrate and/or other iron carriers, the acquisition of heme, the assembly of the type VI secretion system, and amino acid transport. Pathways upregulated in P482 specifically by tomato exudates included nitric oxide detoxification, repair of iron-sulfur clusters, respiration through the cyanide-insensitive cytochrome bd, and catabolism of amino acids and/or fatty acids. The maize-specific response included upregulation of genes associated with motility, the activity of MexE and two other RND efflux pumps, and copper tolerance. To provide more context to the study, we determined the chemical composition of exudates by GC-MS, NMR, and LC-SRM. Our results bring new insight into the host-driven metabolic adaptations of promiscuous root colonizing bacteria.

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Section: Resultsmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Shared and host-specific transcriptomic response of Pseudomonas donghuensis P482 to the exudates of tomato (Dicot) and maize (Monocot) shed light on the host-adaptive traits in the promiscuous root colonizing bacteria

Krzyżanowska¹,

Jablonska²,

Kaczyński

et al. 2022

Preprint

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“…As a vital ECF σ factor in P. aeruginosa , PvdS plays a crucial role in the regulation of pyoverdine biosynthesis. According to previous research, 7-HT—as a newly reported bacterial siderophore with antifungal and nematocidal functions—is a virulence factor of P. donghuensis [ 22 , 23 , 24 , 43 , 46 ]. Therefore, we aimed to focus more attention on some negative regulators in 7-HT synthesis, which, thus, play irreplaceable roles in iron homeostasis.…”

Section: Discussionmentioning

confidence: 99%

Insight into the Global Negative Regulation of Iron Scavenger 7-HT Biosynthesis by the SigW/RsiW System in Pseudomonas donghuensis HYS

Teng

Gao

et al. 2023

IJMS

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7-Hydroxytropolone (7-HT) is a unique iron scavenger synthesized by Pseudomonas donghuensis HYS that has various biological activities in addition to functioning as a siderophore. P. donghuensis HYS is more pathogenic than P. aeruginosa toward Caenorhabditis elegans, an observation that is closely linked to the biosynthesis of 7-HT. The nonfluorescent siderophore (nfs) gene cluster is responsible for the orderly biosynthesis of 7-HT and represents a competitive advantage that contributes to the increased survival of P. donghuensis HYS; however, the regulatory mechanisms of 7-HT biosynthesis remain unclear. This study is the first to propose that the ECF σ factor has a regulatory effect on 7-HT biosynthesis. In total, 20 ECF σ factors were identified through genome-wide scanning, and their responses to extracellular ferrous ions were characterized. We found that SigW was both significantly upregulated under high-iron conditions and repressed by an adjacent anti-σ factor. RNA-Seq results suggest that the SigW/RsiW system is involved in iron metabolism and 7-HT biosynthesis. Combined with the siderophore phenotype, we also found that SigW could inhibit siderophore synthesis, and this inhibition can be relieved by RsiW. EMSA assays proved that SigW, when highly expressed, can directly bind to the promoter region of five operons of the nfs cluster to inhibit the transcription of the corresponding genes and consequently suppress 7-HT biosynthesis. In addition, SigW not only directly negatively regulates structural genes related to 7-HT synthesis but also inhibits the transcription of regulatory proteins, including of the Gac/Rsm cascade system. Taken together, our results highlight that the biosynthesis of 7-HT is negatively regulated by SigW and that the SigW/RsiW system is involved in mechanisms for the regulation of iron homeostasis in P. donghuensis HYS. As a result of this work, we identified a novel mechanism for the global negative regulation of 7-HT biosynthesis, complementing our understanding of the function of ECF σ factors in Pseudomonas.

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“…The composition of the growth medium, especially the carbon source plays an important role in the antagonistic activity of microorganisms, particularly on the production of antibiotics. Glycerol as a sole carbon source suppressed the expression of the gene encoding for the 7-hydroxytropolone (pyoverdine-type siderophore), which is involved in the antimicrobial activity of P. donghuensis P482 ( Matuszewska et al., 2021 ). In contrast, glycerol and glucose promoted the synthesis of the antibiotics 2,4-diacetylphloroglucinol, pyoluteorin, and pyochelin by P. fluorescens CHA0 ( Duffy and Défago, 1999 ).…”

Section: Discussionmentioning

confidence: 99%

Characterization of the antagonistic potential of the glyphosate-tolerant Pseudomonas resinovorans SZMC 25872 strain against the plant pathogenic bacterium Agrobacterium tumefaciens

et al. 2022

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The utilization of microorganisms with biocontrol activity against fungal and bacterial pathogens of plants is recognized as a promising, effective, and environment-friendly strategy to protect agricultural crops. We report the glyphosate-tolerant Pseudomonas resinovorans SZMC 25872 isolate as a novel strain with antagonistic potential towards the plant pathogenic bacterium Agrobacterium tumefaciens. In our studies, the growth of the P. resinovorans SZMC 25872 and A. tumefaciens SZMC 14557 isolates in the presence of 74 different carbon sources, and the effect of 11 carbon sources utilized by both strains on the biocontrol efficacy was examined. Seven variations of media with different carbon sources were selected for the assays to observe the biocontrol potential of the P. resinovorans strain. Also, 50% concentrations of the cell-free culture filtrates (CCF) obtained from medium amended with L-alanine or succinic acid as sole carbon source were found to be effective for the growth suppression of A. tumefaciens by 83.03 and 56.80%, respectively. The effect of 7 media on siderophore amount and the activity of extracellular trypsin- and chymotrypsin-like proteases, as well as esterases were also evaluated. Significant positive correlation was found between the siderophore amount and the percentage of inhibition, and the inhibitory effect of the CCFs obtained from medium amended with succinic acid was eliminated in the presence of an additional iron source, suggesting that siderophores produced by P. resinovorans play an important role in its antagonistic potential. The metabolic profile analysis of the P. resinovorans SZMC 25872 strain, performed by high performance liquid chromatography - high resolution mass spectrometry (HPLC-HRMS), has identified several previously not reported metabolites that might play role in the antagonistic effect against A. tumefaciens. Based on our findings we suggest that the possible inhibition modes of A. tumefaciens SZMC 14557 by P. resinovorans SZMC 25872 include siderophore-mediated suppression, extracellular enzyme activities and novel bioactive metabolites.

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The carbon source-dependent pattern of antimicrobial activity and gene expression in Pseudomonas donghuensis P482

Cited by 20 publications

References 131 publications

Shared and host-specific transcriptomic response of Pseudomonas donghuensis P482 to the exudates of tomato (Dicot) and maize (Monocot) shed light on the host-adaptive traits in the promiscuous root colonizing bacteria

Shared and host-specific transcriptomic response of Pseudomonas donghuensis P482 to the exudates of tomato (Dicot) and maize (Monocot) shed light on the host-adaptive traits in the promiscuous root colonizing bacteria

Insight into the Global Negative Regulation of Iron Scavenger 7-HT Biosynthesis by the SigW/RsiW System in Pseudomonas donghuensis HYS

Characterization of the antagonistic potential of the glyphosate-tolerant Pseudomonas resinovorans SZMC 25872 strain against the plant pathogenic bacterium Agrobacterium tumefaciens

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