The Cation Composition of the Seminal Plasma and Prostatic Fluid and its Correlation to Semen Quality

Homonnai, Z. T.; Matzkin, Haim; Fainman, N; Paz, Gedalia; Kraicer, P. F.

doi:10.1016/s0015-0282(16)43281-4

Cited by 63 publications

(54 citation statements)

References 8 publications

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“…We have used markers to evaluate the function of the accessory glands such as fructose for the seminal vesicle and calcium for the prostate (5). Among the responders, only fructose showed a tendency to decrease reflecting a decreased vesicular function.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Semen Quality following Kallikrein Treatment

Homonnai¹,

Shilon²,

Paz³

1978

Gynecol Obstet Invest

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30 asthenozoospermic subfertile men were treated parenterally for 3 months with kallikrein. 20 men showed a significant increase in sperm motility, 66% (responders), while the other 10 men, 34% (non-responders), did not respond to this treatment. A striking decrease in sperm concentration (by 32%) was found in most patients. No latent period was found following kallikrein treatment. Thus, semen quality did not differ if examined immediately following the cessation of treatment or a few weeks afterwards. A poor pregnancy rate was recorded, i.e. 20% among the responders and 10% among the non-responders. In conclusion, kallikrein is a useful drug in the treatment of asthenozoospermia only when the sperm concentration is not decreased below the optimal value.

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Section: Discussionmentioning

confidence: 99%

Evaluation of Semen Quality following Kallikrein Treatment

Homonnai¹,

Shilon²,

Paz³

1978

Gynecol Obstet Invest

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“…The concentrations of calcium, magnesium, and zinc are highly correlated (Homonnai et al, 1978;Adamopoulos and Deliyiannis, 1983). Studies measuring salt concentrations in semen indicate substantial variation among donors (Girgis et al, 1980).…”

Section: Ionsmentioning

confidence: 99%

“…A measurement of ''viscosity'' is frequently performed as recommended in the WHO Manual (1999), which defines an abnormal viscosity sample as one that can be drawn Arver and Sjoberg, 1982Fong et al, 1986Ford and Harrison, 1984Kilic et al, 1996Magnus et al, 1990Prien et al, 1990 Cl (mg/100 mL) 130 157 151.0 152 112 133 158 Gershbein and Thielen, 1988Hirsch et al, 1991Huggins and Johnson, 1933Huggins et al, 1942Jeyendran et al, 1989Kavanagh, 1985Rosecrans et al, 1987 K (mg/100 mL) 80 125 73.75 129 89.5 Bondani et al, 1973Gershbein and Thielen, 1988Girgis et al, 1980Hirsch et al, 1991Huggins et al, 1942 81.6 91 Jeyendran et al, 1989Kavanagh, 1985Mendiratta et al, 1980Nag and Chaudhuri, 1978Quinn et al, 1965; whole semen 88.0 113 50 126 154 Quinn et al, 1965;plasma Rosecrans et al, 1987Schirren, 1961Sheth and Rao, 1962aSheth and Rao, 196896 113.9 113 Skandhan et al, 1978Skandhan and Mazumdar, 1981Wood et al, 1982 Colleen et al, 1975Homonnai et al, 1978Jeyendran et al, 1989Kavanagh, 1985Papadimas et al, 1983 6.5 9.0 11.3 8.6…”

Section: Viscositymentioning

confidence: 99%

“…Abou-Shakra et al, 1989 Arver and Sjoberg, 1982Carpino and Siciliano, 1998Colleen et al, 1975Cooper et al, 199117.2 16.5 14 16 11.76 Hirsch et al, 1991Homonnai et al, 1978Jeyendran et al, 1989Kavanagh, 1985Lewis-Jones et al, 1996 8.4 14 15.0 Mandal and Bhattacharyya, 1986Mandal and Bhattacharyya, 1987aMandal and Bhattacharyya, 1990Mann and Lutwak-Mann, 1981Marmar et al, 1975; atomic absorption 18.9 13.4 9.26 19.0 16.6 Marmar et al, 1975; neutron activation Mawson and Fischer, 1956Mendiratta et al, 1980Papadimas et al, 1983Paz et al, 197714.64 14.7 69.29 10.6 19.5 Ponchietti et al, 1984Rosecrans et al, 1987Schoenfeld et al, 1979Sorensen et al, 1999Stankovic and Mikac-Devic, 197613 12.4 14.4 Stegmayr et al, 1982Umeyama et al, 1986Wood et al, 1982 Makler et al, 1981Mandal and Bhattacharyya, 1987bPolak and Daunter, 1984Velazquez et al, 1977 Purvis et al, 1986Rosecrans et al, 1987Schirren et al, 1977Schoenfeld et al, 1979225 302 136 Sheth and Rao, 1962bTauber et al, 1975…”

Section: Viscositymentioning

confidence: 99%

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A Review of the Physical and Chemical Properties of Human Semen and the Formulation of a Semen Simulant

Owen

Katz

2005

Journal of Andrology

355

298

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A fluid medium was developed to simulate the salient physical and chemical properties of human semen. The composition of the medium was based upon an extensive review of the literature on constituents of human semen. In choosing the ingredients for this medium, the goal was to emphasize properties that influence interactions of human semen with topical contraceptive, prophylactic, or therapeutic products. Among these properties, pH and buffering capacity, osmolarity, ionic strength, and rheological properties play dominant roles in the physico-chemical processes that govern drug release kinetics and delivery vehicle distribution. W hen therapeutic, contraceptive, or prophylactic formulations are applied to the vagina, they encounter a variety of fluids with widely varying physical and chemical properties. These fluids include those that originate in the vagina and those that flow into it (eg, cervical mucus and semen). The fluid actually present at any location within the vagina is a mixture, to a varying extent, of these fluids. The flow, retention, drug delivery kinetics, and bioactivity of vaginal formulations depend upon their interactions with these resident fluids. Work done by our group has determined that the physical and chemical properties of both the delivery vehicle and the surrounding environment are important factors in determining product performance (Katz et al, 1998;Owen et al, 1999aOwen et al, , 2000Owen et al, , 2001Owen et al, , 2003. Understanding of these interactions can, therefore, aid in the design and development of new and improved formulations.One component of such research is the in vitro testing of formulations with fluids representative of those that will be encountered within the vagina. Our laboratory has been developing and applying in vitro assays that focus on how the deployment and delivery of contraceptive and prophylactic compounds are affected by the properties of the delivery vehicle and its interactions with the surrounding fluids. To develop these assays using standardized materials of sufficient volume, we have found it useful to employ simulants of ambient biological fluids. Our for- mulation of a vaginal fluid simulant has been described in a previous publication (Owen and Katz, 1999b), and our semen simulant (and earlier versions) was presented in a number of studies of contraceptive and microbicidal gels (Owen et al, 2003(Owen et al, , 2004Geonnotti and Katz, 2004;Geonnotti et al, 2005a;Geonnotti et al, 2005b). Here we describe the formulation of a semen simulant embodying salient physical and chemical components and properties of human semen. This is based on a comprehensive review of the literature, and we present here, as well, an updated summary of the constituents of human semen. Materials and MethodsThe quantity and composition of human semen have been studied for a variety of reasons (eg, for the diagnosis of conditions such as prostatitis, infertility, and cancer), and the results of these studies were used in the development of our simulant. It i...

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“…After that, seminal plasma was resuspended in 50 ml 0.5% lanthanun oxide, according to Hommonnai (1978), modified. After digestion reading was done over a 12-to 24-h period in an atomic absorption spectrophotometer (GBC-932AA), at 422.7 nm.…”

Section: Extraction and Detection Of Semen Proteinsmentioning

confidence: 99%

Variations of protein profiles and calcium and phospholipase A2 concentrations in thawed bovine semen and their relation to acrosome reaction

Marques

Goulart

Silva³

2000

Genet. Mol. Biol.

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Just as calcium plays an integral role in acrosome capacitation and reaction, several spermatozoon proteins have been reported as binding to the ovum at fertilization. We examined the relationship between thawed bovine semen protein profiles, seminal plasma calcium ion concentration, spermatozoon phospholipase A2 (PLA2) activity and acrosome reaction. Electrophoretic profile analysis of spermatozoa and bovine seminal plasma proteins (total and membrane) revealed qualitative and quantitative differences among bulls. Variations in PLA2 and seminal plasma calcium concentration indicated genetic diversity among individuals. A 15.7-kDa membrane protein was significantly correlated (r = 0.71) with acrosome reaction, which in turn has been associated with in vivo fertility.
Várias proteínas que constituem o espermatozóide têm sido relatadas como sendo proteínas que se ligam ao óvulo no momento da fertilização, bem como íons cálcio têm um papel importante na capacitação e reação acrossômica. Baseado nisto, este estudo teve como objetivo analisar e correlacionar proteínas do sêmen congelado bovino de diferentes raças, concentração de íons cálcio no plasma seminal e atividade da fosfolipase A2 do espermatozóide com a reação acrossômica, visando encontrar fatores que influenciem no processo de fertilização bovina. Análises do perfil eletroforético das proteínas (totais e de membrana) do espermatozóide e do plasma seminal bovino revelaram variabilidade protéica entre indivíduos na qual diferenças qualitativas e quantitativas foram identificadas. A quantificação da fosfolipase A2, bem como da concentração de cálcio no plasma seminal revelaram diversidade genética entre touros. Uma proteína de 15,7 kDa apresentou correlação significativa (0.71) com a reação acrossômica, que pode estar diretamente relacionada com a fertilização in vivo e deste modo outros experimentos podem ser realizados a fim de investigar a utilidade deste marcador protéico na verdadeira fertilidade

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The Cation Composition of the Seminal Plasma and Prostatic Fluid and its Correlation to Semen Quality

Cited by 63 publications

References 8 publications

Evaluation of Semen Quality following Kallikrein Treatment

Evaluation of Semen Quality following Kallikrein Treatment

A Review of the Physical and Chemical Properties of Human Semen and the Formulation of a Semen Simulant

Variations of protein profiles and calcium and phospholipase A2 concentrations in thawed bovine semen and their relation to acrosome reaction

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