The cell based dressing with living allogenic keratinocytes in the treatment of foot ulcers: a case study

Bayram, Yalcin; Deveci̇, Mustafa; Imirzalioğlu, Nejat; Soysal, Yasemin; Şengezer, Mustafa

doi:10.1016/j.bjps.2005.04.031

Cited by 37 publications

(40 citation statements)

References 18 publications

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“…Microcarriers are useful delivery vehicles for tissue engineering and have indicated their unique advantages for repair of bone and cartilage defects (Malda & Frondoza, 2006). Various microcarriers such as collagen-coated dextran microspheres, poly(l-lactide) (PLLA) microspheres, and gelatin microspheres have achieved good therapeutic effects for treatment of degenerated or wounded tissue (Demetriou et al, 1988), restoration of tissue function (Moscioni et al, 1989), promotion of wound healing (Voigt et al, 1999;Bayram et al, 2005;LaFrance & Armstrong, 1999), and therapy for Parkinson's disease (Liu et al, 2004).…”

Section: (Ii) Microcarriers As Cell Delivery Systemsmentioning

confidence: 98%

Microspheres for Enzyme Immobilization

2013

Microspheres and Microcapsules in Biotechnology

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Section: (Ii) Microcarriers As Cell Delivery Systemsmentioning

confidence: 98%

Microspheres for Enzyme Immobilization

2013

Microspheres and Microcapsules in Biotechnology

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“…In the literature, there are some studies about cell therapy for the treatment of leg ulcers in diabetic patients; however, most of them have the objective of assessing the effect of allogeneic keratinocytes and fibroblasts (Bayram et al 2005;Han et al 2004;Hanft and Surprenant 2002;Harvima et al 1999). Research using autologous keratinocytes (Moustafa et al 2004;Lobmann et al 2003) and fibroblasts (Vojtassák et al 2006;Cavallini 2007) has shown that the autologous transplant may help achieve faster healing from a small skin biopsy, being a good therapeutic option for large diabetic ulcers.…”

Section: Discussionmentioning

confidence: 97%

“…In the literature cell therapy studies for the treatment of chronic leg ulcers of several etiologies are described (Bolívar-Flores and Kuri-Harcuch 1999;Johnsen et al 2005), including those on diabetic feet (Vojtassák et al 2006;Moustafa et al 2004;Lobmann et al 2003;Bayram et al 2005;Han et al 2004;Cavallini 2007;Hanft and Surprenant 2002;Harvima et al 1999).…”

Section: Introductionmentioning

confidence: 98%

“…The use of hyperbaric oxygen, electrical stimulation, laser radiation and exogenous growth factors are also mentioned (Sweitzer et al 2006;Moustafa et al 2004;Bolívar-Flores and Kuri-Harcuch 1999). Advanced techniques derived from progress made in biotechnology, materials research and cell culture, such as the use of skin grafts and cell implants, may be an excellent option for the treatment of diabetic ulcers (Moustafa et al 2004;Lobmann et al 2003;Bayram et al 2005;Han et al 2004).…”

Section: Introductionmentioning

confidence: 98%

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Tissue therapy with autologous dermal and epidermal culture cells for diabetic foot ulcers

et al. 2011

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A great part of diabetic ulcers on the lower extremities have difficult healing and represent the most common cause of non-traumatic amputation In case of patients unresponsive to the classical therapy with debridement, dressings and systemic antibiotic therapy, cell therapy may be an excellent indication. The objective of this study was to assess the efficacy of autologous skin cell (fibroblasts and keratinocytes) implants cultivated ex vivo and applied to long-standing (9-34 years) skin ulcers of five diabetic patients (4 DM2 and 1 DM1) with autologous fibrin glue. There were six ulcers of onset between 4 months and 20 years before and from 4.0 to 36.62 cm(2) in size, located on the lower limbs and unresponsive to the several conventional treatments. Complete healing was observed in five ulcers (83.3%), after 21-120 days. The patient who presented the largest ulcer had partial improvement in 40 days. It is believed that the more distal ulcer location is, the worse is its prognosis. There probably is a correlation between healing time, ulcer size and prior duration. No adverse reactions derived from the treatment occurred. It is concluded that this method is an excellent therapeutic option for diabetic ulcers, allowing faster healing. Its great advantage is being a minimally invasive procedure that can be carried out in an outpatient clinic.

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“…The single published RCT did not report full results [72], but a reduction was claimed in wound area, as well as improvement in the wound bed in the intervention group.…”

Section: Keratinocytesmentioning

confidence: 99%

A systematic review of the effectiveness of interventions to enhance the healing of chronic ulcers of the foot in diabetes

Hinchliffe

Valk

Apelqvist

et al. 2008

Diabetes Metab. Res. Rev.

203

132

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SummaryThe outcome of management of diabetic foot ulcers is poor and there is uncertainty concerning optimal approaches to management. We have undertaken a systematic review to identify interventions for which there is evidence of effectiveness. A search was made for reports of the effectiveness of interventions assessed in terms of healing, ulcer area or amputation in controlled clinical studies published prior to December 2006. Methodological quality of selected studies was independently assessed by two reviewers using Scottish Intercollegiate Guidelines Network (SIGN) criteria. Selected studies fell into the following categories: sharp debridement and larvae; antiseptics and dressings; chronic wound resection; hyperbaric oxygen (HBO); reduction of tissue oedema; skin grafts; electrical and magnetic stimulation and ultrasound. Heterogeneity of studies prevented pooled analysis of results. Of the 2251 papers identified, 60 were selected for grading following full text review. Some evidence was found to support hydrogels as desloughing agents and to suggest that a systemic (HBO) therapy may be effective. Topical negative pressure (TNP) may promote healing of post-operative wounds, and resection of neuropathic plantar ulcers may be beneficial. More information was needed to confirm the effectiveness and cost-effectiveness of these and other interventions. No data were found to justify the use of any other topically applied product or dressing, including those with antiseptic properties. Further evidence to substantiate the effect of interventions designed to enhance the healing of chronic ulcers is urgently needed. Until such evidence is available from robust trials, there is limited justification for the use of more expensive treatments and dressings.

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The cell based dressing with living allogenic keratinocytes in the treatment of foot ulcers: a case study

Cited by 37 publications

References 18 publications

Microspheres for Enzyme Immobilization

Microspheres for Enzyme Immobilization

Tissue therapy with autologous dermal and epidermal culture cells for diabetic foot ulcers

A systematic review of the effectiveness of interventions to enhance the healing of chronic ulcers of the foot in diabetes

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