Microspheres for Enzyme Immobilization

doi:10.1201/b14540-3

Cited by 5 publications

(7 citation statements)

References 107 publications

(93 reference statements)

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“…Microcarriers are typically 100–450 µm in diameter and offers very large surface area per unit culture volume for cell attachment . However, the size distribution of microcarriers should be narrower because it is important for good mixing in a bioreactor, homogenous suspension of microcarriers, equal distribution of cells between microcarriers so that confluence of cells can be reached at about the same time on each microcarrier, and also for equal sedimentation during scale‐up steps in large‐scale processes . Given these reasons, an optimum balance is often achieved when the size of microcarriers is in the narrow 150–230 µm range, which is similar to many commercial microcarriers such as Cytodex (141–248 µm) and SoloHill (150–210 µm) microcarriers.…”

Section: Resultsmentioning

confidence: 99%

Ultraviolet/ozone treated polystyrene microcarriers for animal cell culture

Arifin

Mel

Samsudin

et al. 2015

J. Chem. Technol. Biotechnol.

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Section: Resultsmentioning

confidence: 99%

Ultraviolet/ozone treated polystyrene microcarriers for animal cell culture

Arifin

Mel

Samsudin

et al. 2015

J. Chem. Technol. Biotechnol.

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“…Instead of energy released that favors oxygen degradation, UV irradiation may caused cleavage on the ester linkage of PCL chain. The cleavage contribute to the addition of the oxygen content on the surface of microcarrier and make it more susceptible for gelatin immobilization [10]. The properties of UV/O 3 treated and gelatin immobilized PCL surface were futher characterized to confirm the presence of the functional group and to ensure gelatin was successfully incorporated on the porous PCL microcarrier surface.…”

Section: A Microcarrier Propertiesmentioning

confidence: 99%

Surface Modification of Microcporous of Polycaprolactone (PCL) Microcarrier to Improve Microcarrier Biocompatibility

Samsudin

Hashim

Arifin

et al. 2018

Int. J. Adv. Sci. Eng. Inf. Technol.

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Oil/water emulsion solvent evaporation method was employed to fabricate polycaprolactone (PCL) microcarriers. In order to produce porous microcarrier, the method was slightly modified. The porous network channels were generated inside the microcarrier by introducing camphene that was dissolved in the same solvent used to dissolve raw PCL. The evaporation of solvent and sublimination of camphene during the process of emulsion solvent evaporation method, produce solidified porous microcarrier beads. The surface porous microcarriers beads were further modified to make it competent for cell attachment and proliferation. The surface of these microcarriers were modified with UV/O 3 system to introduce functional group and charge on the surface. The treatment followed with the immobilization of halal gelatin on the surface to imorove the biocompatibility of the microcarrier. Porous microcarrier with optimal size distribution was successfully fabricated. The average pore size of 11.74±8.32 µm was obtained with the concentration of 20% (w/v) of camphene. The porous PCL microcarrier was further tailored with gelatin through surface treatment with UV/O 3 system and gelatin immobilization and validation of its compatibility towards mammalian cell was tested with cultivation of green monkyer kidney cell (Vero) in the suspension culture. Vero cells attach and proliferatie well on the gelatin coated porous PCL microcarrier with maximum cell number of 3.90 × 10 5 cells/ml as compared to cell proliferation on UV/O 3 treated porous PCL microcarrier (1.83 × 10 5 cells/ml). The developed microcarrier may be potentially applicable as a cell delivery scaffold for cell tissue culture and tissue engineering application.

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“…Both solid-filled and porous microcarriers are used to culture cells (7). Solid-filled microcarriers are suitable for scaling up anchorage-dependent cell cultures but have smaller surface areas than porous microcarriers, exhibit higher shear stress, and can form aggregates (17). Porous microcarriers, on the other hand, have several advantages including their 3D topography with a large surface area allowing the use of fewer microcarriers and therefore, protecting cells from shear stress and damage.…”

Section: Introductionmentioning

confidence: 99%

“…Porous microcarriers, on the other hand, have several advantages including their 3D topography with a large surface area allowing the use of fewer microcarriers and therefore, protecting cells from shear stress and damage. This also allows the use of higher stirring speeds (17,18).…”

Section: Introductionmentioning

confidence: 99%

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3D culture of mouse gastric stem cells using porous microcarriers

et al. 2017

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The lining epithelium of the stomach includes multipotent stem cells which undergo proliferation and migration-associated differentiation. These cells give rise to multiple cell lineages that produce mucus, acid, pepsinogen and various hormones/peptides. A 3D culture for stem cells would facilitate identification of the factors that control proliferation and/or differentiation programs. Here, we report on the use of disk-like ImmobaSil HD silicone-rubber matrix based microcarriers that are permeable to oxygen and reduce the creation of toxic environment within the center of the microcarrierd for culturing the mouse gastric stem (mGS) cells. We define several parameters that affect the initial cell attachment such as size of cell inoculum, serum concentration, mode and speed of agitation. We show that although such a microcarrier allows for attachment and growth of gastric stem cells, it does not lend itself and does not support the functional differentiation of such cells.

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Microspheres for Enzyme Immobilization

Cited by 5 publications

References 107 publications

Ultraviolet/ozone treated polystyrene microcarriers for animal cell culture

Ultraviolet/ozone treated polystyrene microcarriers for animal cell culture

Surface Modification of Microcporous of Polycaprolactone (PCL) Microcarrier to Improve Microcarrier Biocompatibility

3D culture of mouse gastric stem cells using porous microcarriers

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