The Cells of a Normal Lymph Node

McMillan, Donald B.; Engelbert, Vibeke E.

doi:10.1139/z60-065

Cited by 10 publications

(5 citation statements)

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“…In contrast, the limited number of eosinophils found on control slides were not labeled. The Giemsa stain of the autoradiograms allowed for excellent visualization of the characteristic large redorange granules of the eosinophil and the frequency of eosinophils observed in the control node preparations agreed well with that found by M cM illa n and E n g elb er t (21). It has been shown (22) that the number of eosinophils in a lymph node increases markedly with immunization but the role of these cells in the immune response is not clear.…”

Section: Resultssupporting

confidence: 80%

An Appraisal of an Autoradiographic Technique for Enumeration of Antibody Containing Cells in Response to Salmonella Somatic Polysaccharide

Pasanen¹,

Epstein²

1967

Int Arch Allergy Immunol

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Section: Resultssupporting

confidence: 80%

An Appraisal of an Autoradiographic Technique for Enumeration of Antibody Containing Cells in Response to Salmonella Somatic Polysaccharide

Pasanen¹,

Epstein²

1967

Int Arch Allergy Immunol

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“…Unfortunately, the limits between these classes have usually been arbitrarily chosen and have varied among species and among authors (1,2,3,4,5,6,7). The problem of classification by morphology alone has been further complicated by the question of whether to measure a cell's total diameter or its nuclear dia meter, and by the fact that the size of cells in smears is markedly influenced by technique and the presence or absence of erythrocytes (4,8).…”

Section: The Sizes and Interrelations Of Lymphocytes In Thoracicmentioning

confidence: 99%

The Sizes and Interrelations of Lymphocytes in Thoracic Duct Lymph and Lymph Node of Normal and Stimulated Rats

Rieke¹,

Everett²,

Caffrey³

1963

Acta Haematol

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“…on "naked" blast cell nuclei from mesenteric lymph nodes of six rabbits prepared by the imprint technique and stained with the May-Griinwald Giemsa method. Previously, the imprint of the lymph node was shown to consist of three regions differentiated on the basis of numbers and types of cells in each (14). The present analysis was confined to the blast cells of the peripheral region since it contains most of the blast cells of the imprint and to cells devoid of cytoplasm, i.e.…”

Section: Materials and Methods Regarded But There Has Been Renewed Inmentioning

confidence: 97%

“…Only "naked" nuclei of the round to ovoid form, i.e. the long axis was no more than 3 X the short axis, were analyzed since they make up about 77% of all blast cell nuclei (14). Intranuclear vesicles were defined as spherical pale or non-staining areas, 1 p or greater in diameter, and enclosed for more than SOYo of their circumference (17).…”

Section: Materials and Methods Regarded But There Has Been Renewed Inmentioning

confidence: 99%

See 1 more Smart Citation

Protoplasmic fragments in haematopoietic tissues and analysis of intranuclear vesicles in lymph node blast cells of the rabbit

Westermann¹,

Shelley²,

Engelbert³

1970

Can. J. Zool.

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1970. Protoplasmic fragments in haematopoietic tissues and analysis of intranuclear vesicles in lymph node blast cells of the rabbit. Can. J. ZOO^. 48: 709-716. Vesicles and cytoplasmic fragments are found in greatest numbers in the lymph node and spleen and least commonly in the thymus and bone narrow in the rabbit. Vesicles appear to originate by the extrusion of intranuclear and intracytoplasmic vesicles m~s t l y riom cell5 of the lymphoid series. Cy toplasrnic fragments formed by the pinching off of cytoplasmic bud? of blast ancl Iymphoid cells may be cither round, oval, or irregular in shape. Vesicles and cytoplasmic fragments arc abscnl from blood smears and extremely difficult to recognize in sections or in areas of ~mprints here the cells arc closely applied one to another. About one-third of all round to oval "naked" blast cells in imprints of mesenteric lymph nodes containvesicles ranging to 7 pin diameter although most are ahout 1 p in d~arneter. Large "naked" nuclei contain more vesicles than expected although the size of the nucleus dwx not affect the s~zc of the vesicles present. We suggest that chromatin from "naked" nuclei and smaller free chromatin masses may become transferred to free vesicles and this process may function in new cell formation. IntroductionSoderstrom (23) has stressed the importance Observations on living and fixed haematopoietic tissues reveal the presence of many protoplasmic structures representing cell parts in addition to the cells commonly found. These cellular fragments include platelets, red blood corpuscles, RNA vesicles (nucleoli) (1 6), basic nuclear units (organized extruded portions of nuclear chromatin) (8, 9, 10, ll), nuclear protuberations becoming clone cells (21), and detached protoplasmic pieces which are distinguishable from platelets (1 1, 18, 19,23).Protoplasmic shedding by haematopoietic cells has been described by many workers in the past (see review by shields (18)). Generally these protoplasmic fragments have been disof the presenci df lymphoglandular bodies as speciiic features of lymphoglandular tissue and their potential use as-a diagnostic feature for the recognition of lymphomas and ectopic lymphoglandular metaplasia.The present paper is concerned with (1) the morphology of certain free protoplasmic fragments and examination of haemopoietic cells in an attempt to understand the origin of the fragments and (2) an analysis of the size and frequency of occurrence of intranuclear vesicles (17) in blast cells devoid of cytoplasm in the mesenteric lymph nodes of rabbits. Materials and Methods regarded but there has been renewed interest inRabbits of Ontario farm stock were killed either by them since the work of Willia~son (271, Engel-injectioos of air into ear vein or by intram bert (8, 9, lo, (18, 19), and Smith and venous k j~t i o n s of 1.25% Ibatal. Tissues from the Engelbert (21) with special interest in their spleen, thymus, mesenteric lymph node, and bone marrow possible function or functions. The bits of shed were prepared by the gentle to...

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The Cells of a Normal Lymph Node

Cited by 10 publications

References 0 publications

An Appraisal of an Autoradiographic Technique for Enumeration of Antibody Containing Cells in Response to Salmonella Somatic Polysaccharide

An Appraisal of an Autoradiographic Technique for Enumeration of Antibody Containing Cells in Response to Salmonella Somatic Polysaccharide

The Sizes and Interrelations of Lymphocytes in Thoracic Duct Lymph and Lymph Node of Normal and Stimulated Rats

Protoplasmic fragments in haematopoietic tissues and analysis of intranuclear vesicles in lymph node blast cells of the rabbit

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