The cellular events of injured muscle regeneration depend on the nature of the injury

Lefaucheur, Jean Pascal; Sebille, Alain

doi:10.1016/0960-8966(95)00012-c

Cited by 114 publications

(103 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Since muscle regeneration proceeds by activation, proliferation and fusion of satellite cells, 35,45,47,66 our results imply that these cells have not been substantially transduced by electrotransfer in vivo. This is in agreement with the principle of electrotransfer, which claims a relation between cell diameter, voltage intensity, and transduction efficacy.…”

Section: Figure 7 Lack Of Persistence Of Transduced Fibers In An Acutmentioning

confidence: 82%

“…No classical figure of ongoing degeneration-regeneration 25,35 was observed in the muscular tissue at the time (6 days) of analysis in wild-type mouse muscles ( Figure 1C, D). The transduced fibers, which were identified by ␤-gal expression, were not centronucleated ( Figure 1C, D).…”

Section: Resultsmentioning

confidence: 98%

“…The presence of fibers of smaller diameter or irregular shape is also frequently observed in regenerated muscles. 25,35,47,48 In order to mimic an accelerated degeneration of muscle tissue, we injected notexin intramuscularly in one of the two transduced legs of immunosuppressed wild-type animals 4 weeks after the electrotransfer. Immunosuppression was continued for 1 month at which time animals were killed.…”

Section: (D-f) At Higher Magnification Asterisks Localize the Same Fmentioning

confidence: 99%

“…45,46,48 Following degeneration, small mouse muscle regenerate completely within 3 weeks 35 through the activation, proliferation and fusion of satellite cells and further maturation of the new or hybrid muscle fibers. 35,47,66 Upon notexin administration in wild-type mice, we observed that ␤-gal expression was cleared from the degenerated muscle, while it persisted in the nondegenerated muscle. Therefore we concluded that the muscle degeneration is the cause of loss of ␤-gal after gene transfer, and that the sustained expression of the reporter gene is not compatible with acute muscle degeneration.…”

Section: Figure 7 Lack Of Persistence Of Transduced Fibers In An Acutmentioning

confidence: 99%

See 3 more Smart Citations

Electrotransfer of naked DNA in the skeletal muscles of animal models of muscular dystrophies

Vilquin

Kennel²,

Paturneau-Jouas

et al. 2001

Gene Ther

View full text Add to dashboard Cite

The electrotransfer of naked DNA has recently been adapted to the transduction of skeletal muscle fibers. We investigated the short-and long-term efficacy of this methodology in wild-type animals and in mouse models of congenital muscular dystrophy (dy/dy, dy 2J /dy 2J ), or Duchenne muscular dystrophy (mdx/mdx). Using a reporter construct, the short-term efficacy of fiber transduction reached 40% and was similar in wild-type, dy/dy and dy 2J /dy 2J animals, indicating that ongoing muscle fibrosis was not a major obstacle to the electrotransfer-mediated gene transfer. Although the complete rejection of transduced fibers was observed within 3 weeks in the absence of immunosuppression, the persistency was prolonged over 10 weeks when transient or continuous immunosuppressive regimens were used. Using

show abstract

Section: Figure 7 Lack Of Persistence Of Transduced Fibers In An Acutmentioning

confidence: 82%

Section: Resultsmentioning

confidence: 98%

Section: (D-f) At Higher Magnification Asterisks Localize the Same Fmentioning

confidence: 99%

Section: Figure 7 Lack Of Persistence Of Transduced Fibers In An Acutmentioning

confidence: 99%

See 2 more Smart Citations

Electrotransfer of naked DNA in the skeletal muscles of animal models of muscular dystrophies

Vilquin

Kennel²,

Paturneau-Jouas

et al. 2001

Gene Ther

View full text Add to dashboard Cite

show abstract

“…Laminin labelling and basal lamina histological morphology were not affected by notexininduced injury or during subsequent muscle regeneration, which is consistent with previous reports on notexin-induced muscle injury. 13,14 As assessed by a previous report, AAV transduction is not thus limited by basal lamina either in mature or regenerating muscle. 7 Even though the rAAV vectors used in the present study were replication-deficient, vector DNA amplification could have occurred in regenerating muscle if recombinant vector DNA integrated into myoblast genome, which proliferates actively during the early stages of the regenerative process.…”

Section: L) For the Other Sample Injected 5 Days After Necrosis (Animentioning

confidence: 83%

Recombinant adeno-associated virus type 2 mediates highly efficient gene transfer in regenerating rat skeletal muscle

et al. 2002

View full text Add to dashboard Cite

Effect of single and periodic contusion on the ratsoleus muscle at different stages of regeneration

1999

View full text Add to dashboard Cite

This work analyzed the rat soleus muscle after single and recurrent contusions at different stages of regeneration. A noninvasive contusion was produced by a type of drop-mass equipment. The posterior region of the right hind limb received a trauma and both right and left soleus muscles were analyzed 1, 4, and 6 days after a single contusion (1x), and 6 and 30 days after periodic contusions (10x, one trauma per week for 10 weeks). Single contusion: there was no significant difference between right and left soleus muscle weight. All animals showed abundant signs of acute damage in the right soleus. AChE activity was identified in regeneration segments of the right soleus. Periodic contusions: there was an increase in the right soleus muscle weight (alpha = 5%) only in the animals evaluated 6 days after periodic contusions. The right soleus muscle showed a high incidence of chronic signs of damage, such as split fibers and a centralized nucleus, which predominated when compared with the acute signs. Right soleus muscles showed split fibers with AChE activity in both the proximal and middle regions. There was no difference in the incidence of muscle fiber types (I, II, and IIC) between right and left soleus muscles after periodic contusions. Skeletal muscle contusion is common in humans, especially in sport activities, where repetitive traumas are also frequent. The results of this work indicate that despite the regeneration process there is an important change in the morphological aspect of regenerated muscle fibers, which possibly affect muscle performance.

show abstract

The cellular events of injured muscle regeneration depend on the nature of the injury

Cited by 114 publications

References 41 publications

Electrotransfer of naked DNA in the skeletal muscles of animal models of muscular dystrophies

Electrotransfer of naked DNA in the skeletal muscles of animal models of muscular dystrophies

Recombinant adeno-associated virus type 2 mediates highly efficient gene transfer in regenerating rat skeletal muscle

Effect of single and periodic contusion on the ratsoleus muscle at different stages of regeneration

Contact Info

Product

Resources

About