The cessation of breathing in the chicken embryo during cold-hypometabolism

Ide, Ryoji; Ide, Satoko Tomita; Mortola, Jacopo P.

doi:10.1016/j.resp.2017.03.004

Cited by 2 publications

(7 citation statements)

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“…Table 3 presents the average data at the onset of the experiment while breathing air. Values of true V . normalO 2 , fH and fB were in the range of those measured previously at this stage of the hatching process 29,33,37 with fH/fB of ~6. In some cases, the peak–trough difference in instantaneous fH was evident (Figure 2), but in the majority, it was minimal or absent.…”

Section: Resultssupporting

confidence: 79%

“…The statistical comparison of the variability between cardiac and breathing activities was obtained by paired two-tailed t-tests. In all cases, differences were considered statistically significant at p < 0.05. the range of those measured previously at this stage of the hatching process 29,33,37 with fH/fB of ~6. In some cases, the peak-trough difference in instantaneous fH was evident ( Figure 2), but in the majority, it was minimal or absent.…”

Section: Statisticssupporting

confidence: 66%

“…In neonatal rats, hypoxia lowered BRV, 28 a phenomenon attributed to the hypoxic decrease in metabolic rate and the associated lower respiratory 'drive'. 29 In mammals, including humans, the interpretation of the foetal fH and fB responses to hypoxia is complicated by the maternal, placental and uterine factors, which can mask or entirely modify the response of the foetus. The avian embryo at the end of incubation offers the opportunity to investigate the foetal responses to hypoxia, free from the confounding variables characteristic of mammalian preparations.…”

Section: Introductionmentioning

confidence: 99%

“…[35][36][37] Differently, in hypoxia, fB at first increases and eventually decreases as the hypoxia becomes very severe. 29,33 No data are available on cardiac and breathing variability during hypoxia. The present work aims to assess the suitability of the chicken embryo in the IP phase as an animal model to investigate the HRV and BRV responses to moderate and severe hypoxia.…”

Section: Introductionmentioning

confidence: 99%

“…In neonatal rats, hypoxia lowered BRV, 28 a phenomenon attributed to the hypoxic decrease in metabolic rate and the associated lower respiratory ‘drive’. 29…”

Section: Introductionmentioning

confidence: 99%

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Heart and breathing rate variability in the avian perinatal period: The chicken embryo as a model

Tomi

Ide

Mortola

2019

Avian Biology Research

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We used the chicken embryo at the internal pipping phase (just after the onset of pulmonary ventilation) as a model to quantify the changes in heart rate (fH), breathing frequency (fB) and their variabilities (heart rate variability and breathing rate variability) during air breathing (21% O 2) and successive 20-min periods of 15%, 10% and 5% O 2 and posthypoxic recovery. For each condition, and for both fH and fB, variability was quantified by time-domain analysis with five standard criteria; these produced qualitatively similar results, which were combined into a single variability index. In normoxia, breathing rate variability was about five times higher than heart rate variability. With 10% O 2 , the embryo's oxygen consumption ( V O 2) and breathing rate variability decreased while heart rate variability increased. In normoxia, respiratory sinus arrhythmia was recognisable in a minority of embryos; its average value was low (~2%) and decreased further with hypoxia. With very severe hypoxia (5% O 2), in some cases, breathing stopped; when it did not, breathing rate variability was high. Within the 20-min post-hypoxia, all embryos recovered, and almost all parameters (fH, heart rate variability, fB, respiratory sinus arrhythmia and  V O 2) were at the pre-hypoxic values; only breathing rate variability remained low. The possibility of simultaneous measurements of fB and fH makes the avian embryo, close to hatching, a suitable model for the investigations of heart rate variability and breathing rate variability in response to hypoxia during the transition from prenatal to postnatal life.

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Section: Resultssupporting

confidence: 79%

Section: Statisticssupporting

confidence: 66%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…In neonatal rats, hypoxia lowered BRV, 28 a phenomenon attributed to the hypoxic decrease in metabolic rate and the associated lower respiratory ‘drive’. 29…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations