The ChaC family of γ-glutamyl cyclotransferases is required for Leishmania to switch to a slow growth state and for long-term survival of the parasite

Das, Sumit; Panja, Puja; Chowdhury, Gaurab; Biswas, Saroj; Dholey, Yuthika; Adak, Subrata

doi:10.1016/j.jbc.2022.102510

Cited by 3 publications

(3 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The result indicates that apoptotic cell reduces from 34% to 13% in the presence of SB-4 (20 μM) (Figure A,B). Next, the activity of SB-4 was also checked by flow cytometry analysis. , The result indicates that the total live cell percentage increases from 2% to 64% in the presence of SB-4 (20 μM) (Figure C–E). In addition, H 2 O 2 accumulation can cause mitochondrial damage and play a vital role in neuronal cell death in neurodegenerative diseases.…”

Section: Resultsmentioning

confidence: 98%

Power of Dopamine: Multifunctional Compound Assisted Conversion of the Most Risk Factor into Therapeutics of Alzheimer’s Disease

Gharai,

Khan,

Pradhan

et al. 2024

ACS Chem. Neurosci.

View full text Add to dashboard Cite

In Alzheimer's disease (AD), reactive oxygen species (ROS) plays a crucial role, which is produced from molecular oxygen with extracellular deposited amyloid-β (Aβ) aggregates through the reduction of a Cu 2+ ion. In the presence of a small amount of redox-active Cu 2+ ion, ROS is produced by the Aβ-Cu 2+ complex as Aβ peptide alone is unable to generate excess ROS. Therefore, Cu 2+ ion chelators are considered promising therapeutics against AD. Here, we have designed and synthesized a series of Schiff base derivatives (SB) based on 2-hydroxy aromatic aldehyde derivatives and dopamine. These SB compounds contain one copper chelating core, which captures the Cu 2+ ions from the Aβ-Cu 2+ complex. Thereby, it inhibits copper-induced amyloid aggregation as well as amyloid self-aggregation. It also inhibits copper-catalyzed ROS production through sequestering of Cu 2+ ions. The uniqueness of our designed ligands has the dual property of dopamine, which not only acts as a ROS scavenger but also chelates the copper ion. The crystallographic analysis proves the power of the dopamine unit. Therefore, dual exploration of dopamine core can be considered as potential therapeutics for future AD treatment.

show abstract

Section: Resultsmentioning

confidence: 98%

Power of Dopamine: Multifunctional Compound Assisted Conversion of the Most Risk Factor into Therapeutics of Alzheimer’s Disease

Gharai,

Khan,

Pradhan

et al. 2024

ACS Chem. Neurosci.

View full text Add to dashboard Cite

show abstract

“…The glutathione-specific γ-glutamyl cyclotransferase family enzymatically degrade GSH [44]. The amino acids produced can be absorbed by the cells and returned to the cells to synthesize GSH.…”

Section: Gsh Biosynthesis and Metabolismmentioning

confidence: 99%

“…After glomerular filtration, absorbed amino acids are used for protein or GSH resynthesis [51]. The glutathione-specific γ-glutamyl cyclotransferase family enzymatically degrade GSH [44]. The amino acids produced can be absorbed by the cells and returned to the cells to synthesize GSH.…”

Section: Gsh Biosynthesis and Metabolismmentioning

confidence: 99%

Mitochondrial Glutathione in Cellular Redox Homeostasis and Disease Manifestation

Chen,

Wang,

Chang

et al. 2024

IJMS

View full text Add to dashboard Cite

Mitochondria are critical for providing energy to maintain cell viability. Oxidative phosphorylation involves the transfer of electrons from energy substrates to oxygen to produce adenosine triphosphate. Mitochondria also regulate cell proliferation, metastasis, and deterioration. The flow of electrons in the mitochondrial respiratory chain generates reactive oxygen species (ROS), which are harmful to cells at high levels. Oxidative stress caused by ROS accumulation has been associated with an increased risk of cancer, and cardiovascular and liver diseases. Glutathione (GSH) is an abundant cellular antioxidant that is primarily synthesized in the cytoplasm and delivered to the mitochondria. Mitochondrial glutathione (mGSH) metabolizes hydrogen peroxide within the mitochondria. A long-term imbalance in the ratio of mitochondrial ROS to mGSH can cause cell dysfunction, apoptosis, necroptosis, and ferroptosis, which may lead to disease. This study aimed to review the physiological functions, anabolism, variations in organ tissue accumulation, and delivery of GSH to the mitochondria and the relationships between mGSH levels, the GSH/GSH disulfide (GSSG) ratio, programmed cell death, and ferroptosis. We also discuss diseases caused by mGSH deficiency and related therapeutics.

show abstract

Importance of aspartate 4 in the Mg2+ dependent regulation of Leishmania major PAS domain-containing phosphoglycerate kinase

Chowdhury,

Biswas,

Dholey

et al. 2024

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics

View full text Add to dashboard Cite

The ChaC family of γ-glutamyl cyclotransferases is required for Leishmania to switch to a slow growth state and for long-term survival of the parasite

Cited by 3 publications

References 54 publications

Power of Dopamine: Multifunctional Compound Assisted Conversion of the Most Risk Factor into Therapeutics of Alzheimer’s Disease

Power of Dopamine: Multifunctional Compound Assisted Conversion of the Most Risk Factor into Therapeutics of Alzheimer’s Disease

Mitochondrial Glutathione in Cellular Redox Homeostasis and Disease Manifestation

Importance of aspartate 4 in the Mg2+ dependent regulation of Leishmania major PAS domain-containing phosphoglycerate kinase

Contact Info

Product

Resources

About