The characterisation and identification of crop cultivars by electrophoresis

Cooke, R. J.

doi:10.1002/elps.1150050202

Cited by 115 publications

(68 citation statements)

References 102 publications

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“…Although the classical Osborne scheme can be criticised as being insufficiently rigorous in modern terms, it provides a useful way of classifying seed proteins and it is still used by most workers in this field (Cooke 1984). There are thus considered four categories of proteins occurring in seeds: 1) albumins, which are soluble in water and comprise mostly enzymatic proteins; 2) globulins, which are soluble in dilute salt solutions and generally occur in protein bodies (i. e., they can be considered as storage proteins in the strict sense); 3) prolamins, which are soluble in aqueous ethanol solutions and are also found in protein bodies as true storage proteins; 4) glutelins, which are soluble in alkaline or acid solutions, or in detergents and are probably mainly structural proteins, although some of them may have metabolic functions.…”

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confidence: 99%

Proteomic analysis of different extracts from barley grains

Chmelı́k¹,

Řehulka²,

Střelcová³

et al. 2002

Plant Soil Environ.

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Identification of several proteins extracted with various solvents from barley grains was performed by the proteomic strategy based on a combination of gel electrophoresis, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and bioinformatics. The mass spectrometry-based strategy designed for protein identification exhibits good sensitivity and rapid protein identification in comparison to other methods. In order to simplify the protein mixture extracted from grains, we used sequential extractions of grains with various solvents (based on modified Osborne system). Several proteins (e.g. β-amylase, B3-hordein) were identified in this way. It seems that this approach can become an important tool for proteomics of cereals.

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mentioning

confidence: 99%

Proteomic analysis of different extracts from barley grains

Chmelı́k¹,

Řehulka²,

Střelcová³

et al. 2002

Plant Soil Environ.

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“…The SDS-PAGE is a promising tool for distinguishing cultivars of a particular crop species (Cooke, 1984;Gardiner and Forde, 1988). The present study was therefore undertaken in order to distinguish among the five given cultivars of mungbean through the use of SDS-PAGE.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of Genetic Diversity in Vigna radiata (L.) Using Protein Profiling and Molecular Marker (RFLP)

Islam¹,

Mir²,

Kudesia³

2015

International J. of Plant Breeding and Genetics

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Seed storage protein profiling through SDS-PAGE and DNA Restriction Fragment Length Polymorphism (RFLPs) were surveyed in 5 accessions of mungbean (Vigna radiata L.) sampled from NBPGR Thrissur, Kerala, India, to evaluate genetic diversity. The protein profile showed a low level of polymorphism (26.66%). The similarity index calculated ranged from (42.30-50%). The dendrogram constructed revealed two clusters. Cluster 1st comprises two accessions IC 251432 and IC 251433. Cluster 2nd comprises 3 cultivars viz., IC 251431, IC 251430 and IC 251434. Cluster 2nd also revealed a 100% homology between IC 251431 and IC 251430 accessions. The RFLP analysis was carried out by single restriction enzyme EcoRI. The RFLP profile revealed a high level of polymorphism (90.90%). A total of 11 clear bands were scored. The only band with fragment size 630 kb was monomorphic while, the rest were polymorphic. Similarity index ranged from (42.82-11.11%). The UPGMA analysis divided the accessions into 2 clusters viz., cluster 1st comprising 4 accessions (IC 251431, IC 251432, IC 251433 and IC 251430). Cluster 2nd includes only one accession (IC 251434) occupying a distinct position in constructed dendrogram.

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“…Tanto a identificação de cultivares como a certificação da pureza genética de sementes de milho são realizadas pelas instituições produtoras de sementes em seus programas de controle de qualidade, principalmente, através de marcadores morfológicos de sementes, plântulas e planta. Porém, o emprego desses marcadores apresenta algumas limitações, como a influência de fatores ambientais e condições do solo (Cooke, 1984;Williams et al, 1993). Para superar tais limitações, técnicas eletroforéticas têm sido empregadas com sucesso na identificação de cultivares e certificação da pureza genética (Goodman & Stuber, 1980;Cardy & Kannenberg, 1982;Arús,1983;Smith & Wych, 1986;Stuber et al, 1988;Martinez, 1990).…”

Section: Introductionunclassified

Alterações dos padrões de isoenzimas em sementes de milho infectadas por fungos

Silva

Pinho

Vieira

et al. 2000

Pesq. agropec. bras.

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RESUMO -Este trabalho teve como objetivo estudar a interferência dos fungos Aspergillus flavus, Fusarium moniliforme e Penicillium spp. sobre padrões eletroforéticos das sementes de milho. Tais padrões são, normalmente, utilizados na identificação de cultivares e na certificação da pureza genética da espécie em estudo. Sementes da cultivar C-805 foram infectadas artificialmente com os referidos fungos; outra parte delas foi tratada com Benomil e Thiabendazol, e ainda outra parte (controle) não foi tratada. As amostras foram acondicionadas em câmara de crescimento (25 o C, 95% de umidade relativa) por um período de 30 dias. Na análise eletroforética foi avaliada também uma amostra de sementes que não permaneceu em câmara de crescimento, visando detectar possíveis interferências das condições do ambiente de crescimento sobre os padrões eletroforéticos. Os resultados obtidos permitiram concluir que a infecção das sementes com os fungos Aspergillus flavus, Fusarium moniliforme e Penicillium spp. promove alterações nos padrões eletroforéticos das isoenzimas malato-desidrogenase, esterase, fosfatase ácida, peroxidase e glutamato-oxalacetato-transaminase. A infecção das sementes com Aspergillus flavus promove alterações tanto na intensidade como no número de bandas dos padrões isoenzimáticos da álcool-desidrogenase e malato-desidrogenase.Termos para indexação: infecção, eletroforese, fisiologia vegetal, pureza. ALTERATION OF THE ISOENZYMES PATTERNS IN CORN SEEDS INFECTED BY FUNGIABSTRACT -This work aimed at studying the interference of the fungi Aspergillus flavus, Fusarium moniliforme and Penicillium spp. on the electrophoretic patterns of corn seeds. Normally, these patterns are used in the identification of cultivars and certification of genetic purity of this species. Seeds of the cultivar C-805 were artificially inoculated with the referred fungi; part was treated with Benomyl and Thiabendazole and part was untreated. All seed samples were stored in growing chamber incubator at 25°C and 95% of relative humidity for 30 days. In the electrophoretic analysis, a seed sample was also evaluated which had not been kept in growing chamber incubator, in order to detect possible interferences of the growing chamber environment on the electrophoretic patterns. The results allowed to conclude that the seed infection by the Aspergillus flavus, Fusarium moniliforme and Penicillium spp. fungi promotes alterations in the electrophoretic patterns of the malate-dehydrogenase, esterase, acid phosphatase, peroxidase and glutamate-oxalacetate-transaminase isoenzymes. Infection by Aspergillus flavus caused considerable alteration in both intensity and number of bands of the isoenzymatic patterns of alcohol-dehydrogenase and malate-dehydrogenase.Index terms: infection, electrophoresis, plant physiology, purity. INTRODUÇÃOA qualidade das sementes é fator importante em programas de produção agrícola, visto que as características agronômicas das cultivares obtidas pela pesquisa chegam aos agricultores através da semente. Esta pode ser anal...

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The characterisation and identification of crop cultivars by electrophoresis

Cited by 115 publications

References 102 publications

Proteomic analysis of different extracts from barley grains

Proteomic analysis of different extracts from barley grains

Evaluation of Genetic Diversity in Vigna radiata (L.) Using Protein Profiling and Molecular Marker (RFLP)

Alterações dos padrões de isoenzimas em sementes de milho infectadas por fungos

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