The characteristics of human cranial bone marrow mesenchymal stem cells

Shinagawa, Katsuhiro; Mitsuhara, Takafumi; Okazaki, Taro; Takeda, Masaaki; Yamaguchi, Satoshi; Magaki, Takuro; Okura, Yunosuke; Uwatoko, Hiroyuki; Kawahara, Yumi; Yuge, Louis; Kurisu, Kaoru

doi:10.1016/j.neulet.2015.08.056

Cited by 25 publications

(24 citation statements)

References 9 publications

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“…It has been reported that MSCs can be derived from cranial bone marrow and cranial suture mesenchyme . However, the human primary cranial suture cells have limited life span in vitro and may lose osteoblastic potential after certain numbers of passages .…”

Section: Discussionmentioning

confidence: 99%

“…The cranial suture complex is a heterogeneous tissue consisting of osteogenic progenitor cells and MSCs from bone marrow and suture mesenchyme . MSCs are multipotent stem cells that are able to differentiate into multiple lineages, including osteogenic, chondrogenic and adipogenic lineages, and have been isolated from numerous tissues including bone marrow, periosteum, liver and adipose .…”

Section: Introductionmentioning

confidence: 99%

“…MSCs are multipotent stem cells that are able to differentiate into multiple lineages, including osteogenic, chondrogenic and adipogenic lineages, and have been isolated from numerous tissues including bone marrow, periosteum, liver and adipose . MSCs can be isolated from cranial bone marrow and cranial suture mesenchyme , as well as human primary cranial suture cells .…”

Section: Introductionmentioning

confidence: 99%

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BMP9 induces osteogenesis and adipogenesis in the immortalized human cranial suture progenitors from the patent sutures of craniosynostosis patients

Song

Zhang

Reid

et al. 2017

J Cellular Molecular Medi

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The cranial suture complex is a heterogeneous tissue consisting of osteogenic progenitor cells and mesenchymal stem cells (MSCs) from bone marrow and suture mesenchyme. The fusion of cranial sutures is a highly coordinated and tightly regulated process during development. Craniosynostosis is a congenital malformation caused by premature fusion of cranial sutures. While the progenitor cells derived from the cranial suture complex should prove valuable for studying the molecular mechanisms underlying suture development and pathogenic premature suture fusion, primary human cranial suture progenitors (SuPs) have limited life span and gradually lose osteoblastic ability over passages. To overcome technical challenges in maintaining sufficient and long‐term culture of SuPs for suture biology studies, we establish and characterize the reversibly immortalized human cranial suture progenitors (iSuPs). Using a reversible immortalization system expressing SV40 T flanked with FRT sites, we demonstrate that primary human suture progenitor cells derived from the patent sutures of craniosynostosis patients can be efficiently immortalized. The iSuPs maintain long‐term proliferative activity, express most of the consensus MSC markers and can differentiate into osteogenic and adipogenic lineages upon BMP9 stimulation in vitro and in vivo. The removal of SV40 T antigen by FLP recombinase results in a decrease in cell proliferation and an increase in the endogenous osteogenic and adipogenic capability in the iSuPs. Therefore, the iSuPs should be a valuable resource to study suture development, intramembranous ossification and the pathogenesis of craniosynostosis, as well as to explore cranial bone tissue engineering.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

BMP9 induces osteogenesis and adipogenesis in the immortalized human cranial suture progenitors from the patent sutures of craniosynostosis patients

Song

Zhang

Reid

et al. 2017

J Cellular Molecular Medi

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“…Interestingly, hOPs also express nestin and vimentin. Beyond its original discovery as a neuroepithelial marker, 44 nestin has been shown to be expressed also in hBMSCs 45,46 and hADSCs 12 . Recently, nestin expression has been associated with a proangiogenic, multipotent population of BMSCs capable of self‐renewal and osteochondral differentiation in a mouse model 47 .…”

Section: Discussionmentioning

confidence: 99%

Three-dimensional environment and vascularization induce osteogenic maturation of human adipose-derived stem cells comparable to that of bone-derived progenitors

Ibrahim

Rodríguez-Flórez

Gardner

et al. 2020

Stem Cells Translational Medicine

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While human adipose-derived stem cells (hADSCs) are known to possess osteogenic differentiation potential, the bone tissues formed are generally considered rudimentary and immature compared with those made by bone-derived precursor cells such as human bone marrow-derived mesenchymal stem cells (hBMSCs) and less commonly studied human calvarium osteoprogenitor cells (hOPs). Traditional differentiation protocols have tended to focus on osteoinduction of hADSCs through the addition of osteogenic differentiation media or use of stimulatory bioactive scaffolds which have not resulted in mature bone formation. Here, we tested the hypothesis that by reproducing the physical as well as biochemical bone microenvironment through the use of three-dimensional (3D) culture and vascularization we could enhance osteogenic maturation in hADSCs. In addition to biomolecular characterization, we performed structural analysis through extracellular collagen alignment and mineral density in our bone tissue engineered samples to evaluate osteogenic maturation. We further compared bone formed by hADSCs, hBMSCs, and hOPs against mature human pediatric calvarial bone, yet not extensively investigated. Although bone generated by all three cell types was still less mature than native pediatric bone, a fibrin-based 3D microenvironment together with vascularization boosted osteogenic maturation of hADSC making it similar to that of bone-derived osteoprogenitors. This demonstrates the important role of vascularization and 3D culture in driving osteogenic maturation of cells easily available but constitutively less committed to this lineage and suggests a crucial avenue for recreating the bone microenvironment for tissue engineering of mature craniofacial bone tissues from pediatric hADSCs, as well as hBMSCs and hOPs.

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“…In the cranial bone marrow, there are also neural-crest derived stem cells, which might give rise to Ewing's sarcoma in the cranial bone marrow. [ 22 ] The periosteal and meningeal dura are present on the inner surface of the cranial bone. Similar to the pericranium, the dura mater has been reported to induce osteogenesis and to have stem cells, which suggests that dura mater stem cells might give rise to intracranial Ewing's sarcoma.…”

Section: Discussionmentioning

confidence: 99%

Primary pericranial Ewing′s sarcoma on the temporal bone: A case report

et al. 2016

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Background:Primary Ewing's sarcoma originating in the pericranium is an extremely rare disease entity.Case Description:A 9-year-old female patient was admitted to our department due to a left temporal subcutaneous mass. The mass was localized under the left temporal muscle and attached to the surface of the temporal bone. Head computed tomography revealed a mass with bony spicule formation on the temporal bone, however, it did not show bone destruction or intracranial invasion. F-18 fluorodeoxyglucose positron emission tomography showed no lesions other than the mass on the temporal bone. Magnetic resonance imaging showed that the mass was located between the temporal bone and the pericranium. The mass was completely resected with the underlying temporal bone and the overlying deep layer of temporal muscle, and was diagnosed as primary Ewing's sarcoma. Because the tumor was located in the subpericranium, we created a new classification, “pericranial Ewing's sarcoma,” and diagnosed the present tumor as pericranial Ewing's sarcoma.Conclusion:We herein present an extremely rare case of primary pericranial Ewing's sarcoma that developed on the temporal bone.

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The characteristics of human cranial bone marrow mesenchymal stem cells

Cited by 25 publications

References 9 publications

BMP9 induces osteogenesis and adipogenesis in the immortalized human cranial suture progenitors from the patent sutures of craniosynostosis patients

BMP9 induces osteogenesis and adipogenesis in the immortalized human cranial suture progenitors from the patent sutures of craniosynostosis patients

Three-dimensional environment and vascularization induce osteogenic maturation of human adipose-derived stem cells comparable to that of bone-derived progenitors

Primary pericranial Ewing′s sarcoma on the temporal bone: A case report

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