The Characterization of Nuclear-Dna Content, the Proliferative Activity and the Immunohistochemical Expression of Gfap, Vim, Leu-7, S-100, P53 and Cathepsin-D in Human Glioblastoma Multiformes (Hgbms) Versus Human GBM Cell-Lines Grafted Into the Brains of Nude-Mice

Cathepsin D Is a Potential Serum Marker for Poor Prognosis in Glioma Patients

“…In an experimental mouse model of the human glioblastoma, an association between the cathepsin D immunoreactivity and the aggressive proliferative activity of U87 cells was noticed as compared with the less aggressive U373 cell line (18). These data are in accordance with our result.…”

“…Some authors have reported that overexpression of cathepsin D in the carcinoma cells of the breast and some other organs is associated with a higher risk of relapse and metastasis, and consequently with a shortened survival period (11,12). For brain tumors, there are a few reports indicating the correlation between cathepsin D expression and the biological features of the tumor cells (16)(17)(18). Immunochemical studies of tissue extracts from glioblastomas showed increased levels of cathepsin D as compared with the other grade gliomas and normal brain tissues.…”

Molecular Mechanisms of Fenofibrate-Induced Metabolic Catastrophe and Glioblastoma Cell Death

“…In addition, results in Fig. 9F demonstrate that the primary human GBM cells used in this experiment are positive for the marker glial fibrillary acidic protein (GFAP), which is abundantly expressed in normal astrocytes and glial tumors but is frequently lost in human glioblastoma cell lines, including U-87MG (41)(42)(43). Although these primary GBM cells were more similar to NHA with respect to GFAP expression (Fig.…”

Gastrin induces over-expression of genes involved in human U373 glioblastoma cell migration

“…Since we had observed that gastrin is able to signi®cantly modulate the migration of human glioblastoma cells (DeHauwer et al, 1998), in our present study we have investigated some of the molecular mechanisms by which gastrin could modulate this glioblastoma cell motility. We chose the U373 human glioblastoma cell line as an experimental model because we have already shown that close biological similarities exist between this experimental glioblastoma and highgrade human astrocytic tumors obtained directly from surgical resections (Kruczynski et al, 1995;Camby et al, 2001). We made use of the PCR-subtraction hybridization technique to clone genes di erentially over-expressed in gastrin-treated U373 cells as compared to untreated cells.…”