Addis and his collaborators were the first to examine in some detail the relationship between dietary protein intake and the protein content of the liver. Addis, Lee, found that liver protein increased with rising casein intake. Yuen, Poo, showed that serum albumin, serum globulin or yeast protein were less effective in raising liver protein than colostrum protein, casein and lactalbumin which, in turn, were inferior to liver and kidney proteins. showed that the quantity of cytoplasm present in the liver cell was dependent on the quality and quantity of dietary protein, and suggested that the estimation of labile liver cytoplasm may lend itself to a rapid assessment of the biological or nutritive values of proteins. Since that time, various procedures which may achieve this have been examined in detail with successful results (Kosterlitz, 1944-6; Kosterlitz & Campbell, 1945. Harrison & Long (1945) also followed up this suggestion and fully confirmed and extended the data given in our previous papers. Their, method was based on the regeneration of protein of rats' livers following a 48 hr. fast.
METHODSAnalytical method8. These have already been described in a previous paper (Kosterlitz, 1947).Experimental procedures. For each experiment 4 or 5 female rats, 3-75A45 months old, were selected from the colony. Their average body weight was 221 g., ranging between 181 and 260 g. The mean body weight of each group of 4 or 5 rats varied between 210 and 230 g. If two proteins were compared with each other, litter mates were equally distributed between the two test diets; if a protein was compared with casein, for which a regression line had been constructed, not more than 2 rats of any one litter were placed on the test diet, in order to prevent the results from being unduly biased by a particular litter.Three different procedures were employed. In procedure 1 the rats were transferred from the stock diet directly to the test diet which was given for 7 days. The basal diet consisted of 2 % agar, 3 % salts, 25 % sucrose, 60 % potato starch and 10 % lard (Kosterlitz, 1947). The lard was supplemented with vitamins A, D, and E, and the B vitamins were given partly in the form of the pure chemical compounds and partly as liver concentrate. In procedure 2 the rats were given the PH. CVII.
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