The chemical environmental pollutants BPA and BPS induce alterations of the proteomic profile of different phenotypes of human breast cancer cells: A proposed interactome

Segovia-Mendoza, Mariana; León, Carmen T. Gómez de; Garcı́a-Becerra, Rocı́o; Ambrosio, Javier R.; Nava-Castro, Karen Elizabeth; Morales‐Montor, Jorge

doi:10.1016/j.envres.2020.109960

Cited by 28 publications

(17 citation statements)

References 62 publications

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“…Worldwide, their presence has expanded into different environmental contexts, including food, air, and water, representing a meaningful health issue [5]. Both phthalates and bisphenols have demonstrated a positive correlation with BC development in animal models [8][9][10][11] and with the proliferation of BC cells [12][13][14][15]. Phthalates and bisphenols are implicated in activating different cell signaling pathways through the stimulation of different steroid receptors, especially the ER, characteristic for which they have been called endocrine-disrupting compounds (EDCs) [16,17].…”

Section: Introductionmentioning

confidence: 99%

Association of Serum Levels of Plasticizers Compounds, Phthalates and Bisphenols, in Patients and Survivors of Breast Cancer: A Real Connection?

Segovia-Mendoza

Palacios-Arreola

Monroy-Escamilla

et al. 2022

IJERPH

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Phthalates and bisphenols are ubiquitous environmental pollutants with the ability to perturb different systems. Specifically, they can alter the endocrine system, and this is why they are also known as endocrine-disrupting compounds (EDCs). Interestingly, they are related to the development and progression of breast cancer (BC), but the threshold concentrations at which they trigger that are not well established. Objectives: The aim of this study was to compare the concentration measures of parent EDCs in three groups of women (without BC, with BC, and BC survivors) from two urban populations in Mexico, to establish a possible association between EDCs and this disease. We consider the measure of the parent compounds would reflect the individual’s exposure. Methods: The levels of di-ethyl-hexyl-phthalate (DEHP), butyl-benzyl-phthalate (BBP), di-n-butyl phthalate (DBP) and di-ethyl-phthalate (DEP), bisphenol A (BPA) and bisphenol S (BPS) were determined by gas chromatograph-mass spectrometry in 102 subjects, including 37 women without any pathological disease, 46 patients with BC and 19 women survivals of BC of Mexico and Toluca City. Results: All phthalates were detected in 100% of women, two of them were significantly higher in patients with different BC subtypes in Mexico City. Differential increases were observed mainly in the serum concentration of phthalates in women with BC compared to women without disease between Mexico and Toluca City. In addition, when performing an analysis of the concentrations of phthalates by molecular type of BC, DEP and BBP were found mainly in aggressive and poorly differentiated types of BC. It should be noted that female BC survivors treated with anti-hormonal therapy showed lower levels of BBP than patients with BC. BPA and BPS were found in most samples from Mexico City. However, BPS was undetectable in women from Toluca City. Discussion: The results of our study support the hypothesis of a positive association between exposure to phthalates and BC incidence.

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Section: Introductionmentioning

confidence: 99%

Association of Serum Levels of Plasticizers Compounds, Phthalates and Bisphenols, in Patients and Survivors of Breast Cancer: A Real Connection?

Segovia-Mendoza

Palacios-Arreola

Monroy-Escamilla

et al. 2022

IJERPH

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“…This agrees with our previous work, in which neonatal exposure to BPA modulated macrophage genes, which are involved in the promotion of the alternative phenotype with important implications in metastasis. In addition, the stimulating role of VEGF by different disrupting compounds, including BPA, have also been recently reported in BC cells [6,7].…”

Section: Discussionmentioning

confidence: 84%

“…In this biological mechanism, immunological factors, particularly cytokines, play a fundamental role [5]. In addition, several environmental factors have been linked to an increased incidence of BC and the promotion of metastasis [6,7]. Environmental pollution has gained a lot of attention related to BC.…”

Section: Introductionmentioning

confidence: 99%

Environmental Pollution and Breast Cancer: The Microplastic Component BPA Regulates the Intratumoral Immune Microenvironment and Increases Lung Metastasis

Palacios-Arreola¹,

Nava-Castro²,

Mendoza-Moreno³

et al. 2021

Preprint

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Background: Metastasis is a complex process that involves the spread of the tumor to distant parts of the body from its original site. Metastatic dissemination represents the main physiopathology of cancer. Soluble factors such as cytokines have been closely related to breast cancer (BC) metastasis. Bisphenol A (BPA) is an endocrine disrupting chemical compound with estrogenic properties, which exposure in the early stages of neonatal life leads to an increase in the size and weight of breast tumors and cellular changes in the tumoral immune microenvironment. Methods: Thus, we used female BALB/c mice that were exposed neonatally to a single dose of BPA. Once sexual maturity was reached, a mammary tumor was induced injecting 4T1 cells in situ. After 25 days of injection, we evaluated endocrine alterations, cytokine expression, tissue alterations denoted by macro and micro metastases in the lung, and metastasis-induced cell infiltration. Results: BPA neonatal treatment did not show significant endocrine alterations. Nevertheless, BPA induced a great rate of metastasis to the lung associated with higher intratumoral expression of IL-1b, IL-6, IFN-g, TNF-a and VEGF. Conclusions Our data suggest that cytokines are key players in BC metastasis induction, and that BPA is a risk factor to be considered. This knowledge must be considered with the aim of recognizing environmental pollution in the clinical history of patients to possibly counter BC metastases.

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“…BPA exposure to female rats and histological parameters of both testis and epididymis revealed prominent changes in the reproductive tissues, which suggested that different concentrations of BPA led to marked alterations in the development of the male reproductive system (Ullah et al, 2019). Moreover, studies showed that BPA exposure can contribute to breast cancer development and progression (Segovia-Mendoza et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of BPA effects on autophagy in Neuro-2a cells

Yin

Hua

et al. 2022

Toxicol Ind Health

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Bisphenol A (BPA), which is used for the industrial production of polycarbonate plastics and epoxy resins, is found in many commercially available products. Plasticizer BPA produces chemical substances worldwide, and knowledge of its effects on humans and animals is increasing. In the present work, the morphology of cells was observed by optical microscopy and phalloidin staining to evaluate the toxic effect of BPA on Neuro-2a cells. Autophagy has an important role in the regulation of cell metabolism. To study the effect of BPA on the autophagy in Neuro-2a cells, the expression distribution of LC3 was detected by immunofluorescence, and the expression levels of p62 and Beclin1 were determined using western blot and quantitative real-time PCR (qRT-PCR), respectively. Optical microscopy and phalloidin staining revealed that the cells became rounded and small and that the dendritic spine of the cells were reduced at high BPA doses. Immunofluorescence analysis demonstrated that the expression of LC3 fluorescence intensity was weak at increasing BPA concentrations. Western blot results showed that the relative expression of protein p62 increased significantly and that the relative expression levels of the Beclin1 and the LC3 proteins significantly decreased with increasing BPA concentration. qRT-PCR results showed that the relative expression level of autophagy-related p62 mRNA increased significantly and that the relative expression level of Beclin1 mRNA decreased significantly with increasing BPA concentration. The above results indicated that BPA treatment exerted dose-dependent toxic effects on Neuro-2a cells, and BPA inhibited the autophagy level of Neuro-2a cells, thereby providing a new perspective in studying the toxic effect of BPA on Neuro-2a cells.

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The chemical environmental pollutants BPA and BPS induce alterations of the proteomic profile of different phenotypes of human breast cancer cells: A proposed interactome

Cited by 28 publications

References 62 publications

Association of Serum Levels of Plasticizers Compounds, Phthalates and Bisphenols, in Patients and Survivors of Breast Cancer: A Real Connection?

Association of Serum Levels of Plasticizers Compounds, Phthalates and Bisphenols, in Patients and Survivors of Breast Cancer: A Real Connection?

Environmental Pollution and Breast Cancer: The Microplastic Component BPA Regulates the Intratumoral Immune Microenvironment and Increases Lung Metastasis

Evaluation of BPA effects on autophagy in Neuro-2a cells

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