The Chemistry of Pectic Materials

Hirst, E. L.; Jones, J. K. N.

doi:10.1016/s0096-5332(08)60012-0

Cited by 39 publications

(10 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…8 % of the arabinogalactan-peptide and for as much as 15-20% of the galactan-peptide. Nevertheless, the value of [a]20 for the galactan-peptide provides strong evidence that galactose is present as fl-D-galactosyl residues (Hirst & Jones, 1946;Timell, 1965;Bailey, 1965). Fig.…”

Section: Optical Rotatory Dispersion Andspecific Optical Rotationmentioning

confidence: 99%

Chemical and physical properties of an arabinogalactan-peptide from wheat endosperm

Fincher¹,

Sawyer²,

Stone³

1974

Biochemical Journal

144

View full text Add to dashboard Cite

1. An arabinogalactan-peptide from wheat endosperm was studied by using physicochemical techniques and some aspects of its chemical structure were determined. 2. The arabinogalactan-peptide is a non-associating, polydisperse macromolecule ([unk]=22000) which exhibits only minor non-ideal effects in aqueous solution. 3. Examination of the products of partial acid hydrolysis of the polysaccharide component showed that arabinose is present in the alpha-l-arabinofuranosyl configuration, and i.r.-absorption spectroscopy and optical-rotation studies suggest that the d-galactopyranose residues are linked by glycosidic linkages in the beta-anomeric configuration. 4. The arabinogalactan is linked to a peptide which represents 8% (w/w) of the arabinogalactan-peptide and which may be present as a molecular core. Partial degradation of the polymer by successive treatment with oxalic acid and NaOH showed that the linkage between polysaccharide and peptide involves galactose and hydroxyproline residues and is glycosidic in nature. A tentative model is proposed for the structure of the wheat endosperm arabinogalactan-peptide. 5. The subcellular location and function of the arabinogalactan-peptide is discussed in relation to previous work with related molecules.

show abstract

Section: Optical Rotatory Dispersion Andspecific Optical Rotationmentioning

confidence: 99%

Chemical and physical properties of an arabinogalactan-peptide from wheat endosperm

Fincher¹,

Sawyer²,

Stone³

1974

Biochemical Journal

144

View full text Add to dashboard Cite

show abstract

“…The composition and constitution of pectic substances have been studied extensively and the early literature on the subject has been reviewed by Hirst and Jones (1). Pectins from all sources contain D-galacturonic acid, D-galactose, L-arabinose, and L-rhamnose but i t has not been clear whether these components are joined together in a single polysaccharide or whether a group of polysaccharides is present.…”

mentioning

confidence: 99%

“…Pectins from all sources contain D-galacturonic acid, D-galactose, L-arabinose, and L-rhamnose but i t has not been clear whether these components are joined together in a single polysaccharide or whether a group of polysaccharides is present. Early evidence (1) indicated that the pectic substances were mixtures of three polysaccharides, a galacturonan, a galactan, and an araban. However, subsequent attempts to isolate homoglycans by fractionation of various pectins have not confirmed this interpretation (2)(3)(4)(5)(6)(7)(8).…”

mentioning

confidence: 99%

Fractionation of Pectins From Sunflowers, Sugar Beets, Apples, and Citrus Fruits

Zitko¹,

Bishop²

1965

Can. J. Chem.

View full text Add to dashboard Cite

Pectins from sunflower heads, sugar beet, apple, and citrus fruits were separated into acidinsoluble pectic acids and acid-soluble polysaccharides. The acid-soluble polysaccharides were shown by moving-boundary electrophoresis t o be complex mixtures of four or five polysaccharides. The pectic acids were fractionated by precipitation with sodium acetate from aqueous solution. The fractions were examined for galacturonic acid content, specific rotation, limiting viscosity number, behavior on moving-boundary electrophoresis, and presence of neutral sugars. The results showed that each of the pectic acids contained two acidic components, one a galacturonan free of neutral sugars, the other a galacturonan to which neutral sugars were attached, probably as side chains.The composition and constitution of pectic substances have been studied extensively and the early literature on the subject has been reviewed by Hirst and Jones (1). Pectins from all sources contain D-galacturonic acid, D-galactose, L-arabinose, and L-rhamnose but i t has not been clear whether these components are joined together in a single polysaccharide or whether a group of polysaccharides is present. Early evidence (1) indicated that the pectic substances were mixtures of three polysaccharides, a galacturonan, a galactan, and an araban. However, subsequent attempts to isolate homoglycans by fractionation of various pectins have not confirmed this interpretation (2-8). For example, chromatography on diethylaminoethyl cellulose resolved pectin into a neutral fraction and several acidic fractions (7,8), but each of these fractions still contained units of the same monosaccharides that were present in the original preparation. I t has been known for some time that the predominant structural feature of pectin is a chain of 1 -) 4 linked a-D-galacturonic acid residues (0, 10). There is chemical evidence that in some pectic acids neutral monosaccharides are linked to the basic chain of D-galacturonic acid units (3-6, 11, 12), but the homogeneity of those pectic acids was not established. There have been only txvo reports of the isolation of homoglycans from pectins. Both were galacturonans \vl~ich yielded only D-galacturonic acid on hydrolysis; one was isolated from sunflower heads (13), the other from the bark of amabilis fir (12). The galacturonan from sunflower heads was obtained by extraction with ammonium oxalate:oxalic acid and reprecipitation in 60% ethanol. The galacturonan from the bark of amabilis fir was separated froin another polysaccharide which contained D-galacturonic acid, D-galactose, and L-arabinose by ultracentrifugation of an acidified aqueous solution of the pectin.Precipitation of acidic polysaccharides by electrolytes has been studied extensively with varying results in the fractionations obtained. Fractional precipitation of alginic acid with solutions of potassium chloride and manganous sulphate yielded fractions with different ratios of D-mannuronic and L-guluronic acids (14,15). Most studies of the fractionation of pec...

show abstract

“…Pectin (HIRST and JONES, 1946;WHISTLER and SMART, 1953) is more abundant in bark than in wood where it is formed only during the earlier stages of cell development. Pectin (HIRST and JONES, 1946;WHISTLER and SMART, 1953) is more abundant in bark than in wood where it is formed only during the earlier stages of cell development.…”

Section: Other Wood Polysaccharidesmentioning

confidence: 99%

Chemical Composition of Wood

Côté¹

1968

Principles of Wood Science and Technology

View full text Add to dashboard Cite

The Chemistry of Pectic Materials

Cited by 39 publications

References 26 publications

Chemical and physical properties of an arabinogalactan-peptide from wheat endosperm

Chemical and physical properties of an arabinogalactan-peptide from wheat endosperm

Fractionation of Pectins From Sunflowers, Sugar Beets, Apples, and Citrus Fruits

Chemical Composition of Wood

Contact Info

Product

Resources

About