2022
DOI: 10.1101/2022.01.15.476471
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The Cif proteins from Wolbachia prophage WO modify sperm genome integrity to establish cytoplasmic incompatibility

Abstract: Inherited microorganisms can selfishly manipulate host reproduction to drive through populations. In Drosophila melanogaster, germline expression of the native prophage WO proteins CifA and CifB cause cytoplasmic incompatibility (CI) in which sperms fertilize uninfected embryos that suffer catastrophic mitotic defects and lethality; however in infected females, CifA rescues the embryonic lethality and thus imparts a fitness advantage to Wolbachia. Despite widespread relevance to sex determination, evolution, a… Show more

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Cited by 5 publications
(17 citation statements)
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References 83 publications
(136 reference statements)
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“…In D. melanogaster, dual expression of w Mel CifA with CifB is required to induce CI (Beckmann et al, 2017; Kaur et al, 2022; LePage et al, 2017; Shropshire and Bordenstein, 2019). To examine if T1 w Mel CifA and CifB have any impact on their independent nuclease activities, we co-incubated the proteins with dsDNA or ssRNA substrates as described earlier (Chen et al, 2019).…”
Section: Resultsmentioning
confidence: 99%
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“…In D. melanogaster, dual expression of w Mel CifA with CifB is required to induce CI (Beckmann et al, 2017; Kaur et al, 2022; LePage et al, 2017; Shropshire and Bordenstein, 2019). To examine if T1 w Mel CifA and CifB have any impact on their independent nuclease activities, we co-incubated the proteins with dsDNA or ssRNA substrates as described earlier (Chen et al, 2019).…”
Section: Resultsmentioning
confidence: 99%
“…At the histone-to-protamine transition stage of spermiogenesis, wMel CifA is not highly abundant, and wPip CifA reportedly vanishes (Horard et al, 2022;Kaur et al, 2022). Thus, one explanation is that CifA levels are below the threshold to induce spermatid DNA nicks or it requires co-expression with the CifB binding partner (Wang et al, 2022;Xiao et al, 2021).…”
Section: Discussionmentioning
confidence: 99%
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