The Circular RNA Circ_0085494 Regulates Prostate Cancer Progression Through NRBP1/miR-497-5p Axis

Cao, Chunhui; Sun, Guanghai; Le, Keping; Xu, Qingtao; Li, Chunlin

doi:10.1007/s10528-023-10341-9

Cited by 2 publications

(1 citation statement)

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“…Driven by the progress in highthroughput sequencing and emerging bioinformatics approaches, it has recently been confirmed that circRNAs are widely expressed in mammalian transcriptomes and intricately regulate gene expression and miRNA function [14,15]. Despite their multifaceted roles in cellular physiology, circRNAs have been linked to the pathogenesis and progression of several human diseases [15,16], including reproductive malignancies, as exemplified by circ_0085494 and hsa_circ_0063329 in prostate cancer cells [17][18][19], and circ-MUC16, circRNA-MYLK, and circRNA-UBAP2 in ovarian cancer [20][21][22]. However, it is still unclear how circRNAs regulate ovarian and prostate cancer onset and progression; investigating circRNAs derived from an apoptosis-related genes like BOK in prostate and ovarian cancer cells allows us to explore potential connections between circRNAs and apoptosis in these cancer types.…”

Section: Introductionmentioning

confidence: 99%

Discovery and Comprehensive Characterization of Novel Circular RNAs of the Apoptosis-Related BOK Gene in Human Ovarian and Prostate Cancer Cells, Using Nanopore Sequencing

Kontos,

Hadjichambi,

Papatsirou

et al. 2023

ncRNA

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CircRNAs have become a novel scientific research hotspot, and an increasing number of studies have shed light on their involvement in malignant progression. Prompted by the apparent scientific gap in circRNAs from apoptosis-related genes, such as BOK, we focused on the identification of novel BOK circRNAs in human ovarian and prostate cancer cells. Total RNA was extracted from ovarian and prostate cancer cell lines and reversely transcribed using random hexamer primers. A series of PCR assays utilizing gene-specific divergent primers were carried out. Next, third-generation sequencing based on nanopore technology followed by extensive bioinformatics analysis led to the discovery of 23 novel circRNAs. These novel circRNAs consist of both exonic and intronic regions of the BOK gene. Interestingly, the exons that form the back-splice junction were truncated in most circRNAs, and multiple back-splice sites were found for each BOK exon. Moreover, several BOK circRNAs are predicted to sponge microRNAs with a key role in reproductive cancers, while the presence of putative open reading frames indicates their translational potential. Overall, this study suggests that distinct alternative splicing events lead to the production of novel BOK circRNAs, which could come into play in the molecular landscape and clinical investigation of ovarian and prostate cancer.

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