The classification of hæmolytic streptococci stools of normal pregnant women and of cases of scarlet fever by means of precipitin and biochemical tests

Hare, Ronald; Maxted, W. R.

doi:10.1002/path.1700410315

Cited by 47 publications

(21 citation statements)

References 12 publications

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“…It seems likely, however, that they occur more commonly in the gastrointestinal tract. Haemolytic strains of group F were grown from the faeces of 5 % of normal puerperal women (Hare and Maxted, 1935) and from 11 % of appendices (Rogers, 1957). It would be valuable to elucidate more accurately the distribution in the normal subject of both haemolytic and non-haemolytic strains, thus identifying possible sources of infection.…”

Section: Discussionmentioning

confidence: 99%

Infection with minute-colony-forming beta-haemolytic streptococci.

Poole¹,

Wilson²

1976

J Clin Pathol

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One hundred and thirty-one strains of minute-colony-forming beta-haemolytic streptococci were isolated during the course of routine investigations in clinical bacteriology. Each strain was examined for the presence of polysaccharide antigens of Lancefield's groups A, C, G, and F and characterized in detail as to biochemical and cultural features. On the results of these tests it is concluded that the strains should be placed in the species Streptococcus milleri. The clinical details relevant to the various strains are summarized according to the site of isolation, and their pathological significance is discussed.

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Section: Discussionmentioning

confidence: 99%

Infection with minute-colony-forming beta-haemolytic streptococci.

Poole¹,

Wilson²

1976

J Clin Pathol

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“…They also isolated it from vaginal cultures obtained from women during the puerperium. Other sources from which it has been reported include the skin (Colebrook, Maxted and Johns, 1935) and faeces (Hare and Maxted, 1935).…”

Section: Discussionmentioning

confidence: 99%

Observations on Group-F Streptococci From Human Sources

Wort

1975

Journal of Medical Microbiology

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I N comparison with the vast literature concerning group-A streptococci, our knowledge of group-F streptococci is very meagre and there is little published work concerning them. This report documents a series of 22 strains isolated from children and puerperal women during about 4 years and discusses their significance in human disease. MATERIALS AND METHODSPrimary cultures were inoculated on horse-blood agar (BBL Columbia Base; Becton, Dickinson and Co., Canada, Ltd, Mississauga, Ontario) and incubated at 37°C under aerobic and anaerobic conditions for up to 48 h. Haemolytic colonies were examined microscopically and Gram-positive cocci were inoculated into Todd-Hewitt Broth (BBL). These cultures were incubated at 37°C overnight and used for preparing antigen by the method of Rantz and Randall (1955). Lancefield groups were determined by the microprecipitin technique (Lancefield, 1938) with grouping sera purchased from Burroughs Wellcome Ltd, Beckenham, Kent.Sensitivity to bacitracin (Maxted, 1953) was tested with standard bacitracin disks (BBL Tax0 A) and Columbia horse-blood agar. Some strains were inoculated on to aesculin-bileagar slopes (Difco Aesculin Bile Agar, Difco Laboratories, Detroit, USA) or aesculin-agar slopes. RESULTSDuring the period 1971-74 inclusive, some 3500 strains of haemolytic streptococci were subjected to Lancefield grouping. Of these, 22 strains isolated from 22 different patients belonged to Lancefield's group F. Eight of the strains were from peritoneal-cavity swabs taken at operation, in seven for acute appendicitis and in one for ruptured jejunum; five strains were from the vagina of puerperal women; two isolates were from urine, and one from the perineum of one of these patients was presumably related to the infection of the urine. One strain came from an abscess in the neck of a patient who had a history of dental infection, one from an ear swab from a child with cerebrospinal fluid (CSF) leaking from the ear, and one from a maxillary sinus.All group-F organisms were isolated from mixed cultures and since they were selected for further study because they were haemolytic on horse-blood agar, non-haemolytic strains would not have been recognised. Eight strains were quite fastidious anaerobes, two were microaerophilic, and 12 grew readily and were easily recognised on aerobic culture.Colonial morphology was variable. Nine of the strains grew very slowly, producing the classically described pinpoint colonies and attracting attention because of their haemolysis (Long and Bliss, 1934) but 13 strains produced colonies that were easily visible after overnight incubation. One strain was isolated repeatedly in pure culture from the urine and perineum of a patient who had undergone a renal transplant. Several strains, including this one, grew so slowly in Todd-Hewitt broth that incubation for 5-7 days was required before growth was adequate for the production of grouping antigen. All strains gave clear precipitin

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“…The anatomical distribution of disease associated with these streptococci suggests that a number originate in the gut (Wort, 1975;Parker and Ball, 1976), an observation supported by the isolation of Strep. miller from the normal as well as the inflamed appendix (Poole and Wilson, 1977), and of group F streptococci from faeces (Hare and Maxted, 1935). Their presence on teeth (Phillips et al, 1976;Mejaire and Edwardsson, 1975) and in dental root canals (Ottens and Winkler, 1962) is a potential source of dental sepsis, which in turn has been related to the development of brain abscess (Ingham et al, 1978).…”

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confidence: 99%

Occurrence and cultural features of Streptococcus milleri in various body sites.

Poole¹,

Wilson²

1979

J Clin Pathol

106

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An investigation was made into the habitat of commensal strains of Streptococcus milleri. These showed distinctive patterns of cultural features, dependent on their sites of origin, which were compared with those prevalent in strains grown from the appendix, 'anal' lesions, and Bartholin's abscesses. A biotype, which showed a marked affinity for the vagina and produced acid from raffinose and melibiose, was identified.

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The classification of hæmolytic streptococci stools of normal pregnant women and of cases of scarlet fever by means of precipitin and biochemical tests

Cited by 47 publications

References 12 publications

Infection with minute-colony-forming beta-haemolytic streptococci.

Infection with minute-colony-forming beta-haemolytic streptococci.

Observations on Group-F Streptococci From Human Sources

Occurrence and cultural features of Streptococcus milleri in various body sites.

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