The clinical diagnostic value of plasma miR-592 and miR-217-3p levels in retinoblastoma

Abstract

目的：研究血浆miR-592和miR-217-3p在视网膜母细胞瘤（Rb）中的异常表达，探讨其表达水平对Rb的临床诊断价值。
 方法：选取2018年1月至2019年1月到南昌市洪都中医院就诊的100例Rb患者为Rb组，同期来体检中心就诊的100例健康患者为对照组. 采用实时荧光定量PCR（qRT-PCR）检测所有受试者血浆miR-592和miR-217-3p的表达水平；分析血浆miR-592和miR-217-3p水平与Rb临床病理特征的关系；Pearson 相关分析评估了血浆 miR-592 和 miR-217-3p 水平与总生存率之间的关系。
 结果： Rb组血浆miR-592和miR-217-3p水平显着高于对照组（p < 0.0001），且miR-592的表达与家族遗传史显着相关（p＜ 0.0001)、肿瘤偏倚( p =0.0081)、淋巴结转移( p =0.0048)和病理分级( p =0.0025)，miR-217-3p的表达与家族遗传史显着相关( p＜0.0001)，视神经浸润（p＜0.0001）、淋巴结转移（p = 0.0090）、病理分级（p＜0.0001)。miR-592和miR-217-3p的高表达是Rb更严重的病理表现，随着miR-592（r=-0.2276，p =0.0052）和miR的升高，患者的总生存期显着缩短-217-3p 水平（r=-0.6461，p＜0.0001）。
 结论： miR-592和miR-217-3p在Rb患者血浆中高表达，其升高的水平呈现Rb严重的病理表现和缩短的总生存期，有望成为Rb临床诊断的生物标志物。

Blood miRNAs miR-549a, miR-552, and miR-592 serve as potential disease-specific panels to diagnose colorectal cancer

“…Pan Z. et al have proposed that a high level of miR-592 serves as an indicator of a poorer prognosis in CRC patients [ 33 ]. However, it is worth noting that miR-592 expression has also been observed in other types of cancer, including renal cell carcinoma, prostate cancer, gastric cancer, and retinoblastoma cancer [ [35] , [36] , [37] ]. In 2017, Wang W. discussed the inhibitory effects of miR-592 on the proliferation, migration, and invasion of hepatocellular carcinoma through its targeting of IGF-1R [ 38 ].…”

Study on serum miR-185-5p in assessing the injury severity and prognosis of patients with traumatic brain injury

“…miRNAs can bind to the 3’-UTR by complementary base pairing to inhibit protein transcription or promote mRNA degradation [36] . Studies have shown a correlation between miRNA and the occurrence and development of TBI [37] [38] [39] . It has been found that in the brain tissue of mice within 72 hours after TBI, miR-711 is up-regulated, protein kinase B is targeted to down-regulate, and the activities of FOXO3a/GSK3 and Caspase-3 are enhanced, suggesting that miR-711 could promote neuronal apoptosis after TBI [40] .…”