The cloned avian neurohypophysial hormone receptors

Baeyens, Dennis A.; Cornett, Lawrence E.

doi:10.1016/j.cbpb.2005.09.012

Cited by 45 publications

(33 citation statements)

References 62 publications

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“…The chromosomal location of this opossum V2 receptor gene is therefore still unknown. In the chicken genome database we found the three previously reported receptor genes -V1B (known as VT2 in chicken), V2B (known as VT1) and OTR (known as MTR) [5] -as well as the fourth family member, V1A (sometimes called VT4) ( Table 1). The known V2-type sequence in chicken clusters together with the teleost V2B-type sequences in our phylogenetic tree and no V2A-type sequence could be identified in the chicken genome or in any of the other available avian genomes, namely those of the zebra finch (Taeniopygia guttata), turkey (Meleagris gallopavo) and mallard (Anas platyrhynchos) (data not shown).…”

Section: Ot/vp Receptor Genes In Tetrapodsmentioning

confidence: 99%

“…For example: the chicken vasotocin (vasopressin) receptor initially named VT1 [45] is clearly more similar to mammalian V2, although phylogenetic analyses have placed it outside of the mammalian V2 clade [10,14], and the chicken receptor called VT2 [10] is in fact a homolog of the mammalian V1B, a receptor that has also been known as V3. The cloned chicken receptors are reviewed by Baeyens and Cornett (2006) [5]. In amphibians, vasopressin (vasotocin) receptors showing similarities to mammalian V1A, V1B and V2 as well as oxytocin (mesotocin) receptors have been cloned and characterized in several species [42].…”

Section: Introductionmentioning

confidence: 99%

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The oxytocin/vasopressin receptor family has at least five members in the gnathostome lineage, inclucing two distinct V2 subtypes

Daza¹,

Lewicka²,

Larhammar³

2012

General and Comparative Endocrinology

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Section: Ot/vp Receptor Genes In Tetrapodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The oxytocin/vasopressin receptor family has at least five members in the gnathostome lineage, inclucing two distinct V2 subtypes

Daza¹,

Lewicka²,

Larhammar³

2012

General and Comparative Endocrinology

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“…The V1AR and V1BR are linked to phospholipase C (PLC)/protein kinase C (PKC) signaling pathway while the V2R is coupled to adenylyl cyclase (AC)/protein kinase A (PKA) pathway (Liu and Wess 1996;Gimpl and Fahrenholz 2001). In birds, four VT receptors (VT1, VT2, VT3 and VT4) and one mesotocin receptor (MTR) have been described (Tan et al 2000;Cornett et al 2003;Baeyens and Cornett 2006;Srivastava et al 2008: Selvam et al 2013. But the nomenclature has been inconsistent with those of other gnathostome vertebrates.…”

Section: Introductionmentioning

confidence: 96%

Molecular cloning, sequencing and phylogeny of vasotocin receptor genes in the air-breathing catfish Heteropneustes fossilis with sex dimorphic and seasonal variations in tissue expression

Rawat

Chaube

Joy

2015

Fish Physiol Biochem

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Vasotocin (VT) is the ortholog of vasopressin (VP) in non-mammalian vertebrates and is known for multiple functions. Teleost fishes have a complete repertoire of known VP/VT receptor subtypes (vasopressin type, VR): two V1A subtypes (V1Aa and V1Ab or V1a1 and V1a2) and five V2 subtypes (V2A1, V1A2, V2B1, V2B2 and V2C). Full-length cDNAs of v1a1, v1a2 and v2 (v2a1) with ORFs of 1,308, 1,137 and 1,527 bp, respectively, were cloned and characterized in the catfish Heteropneustes fossilis (Siluriformes, Ostariophysi). BLAST analysis revealed that the genes encoded three VT receptors, V1a1, V1a2 and V2 of 436, 379 and 509 amino acid residues, respectively. The predicted proteins showed typical features of the seven-transmembrane domain receptor core structure with hallmark triplets Asp-Arg-Tyr/Asp-Arg-His (DRY/DRH) and the variable intracellular loop III of vertebrate neurohypophysial hormone receptors. Phylogenetic analysis of the deduced protein sequences revealed that they clustered with the V1Aa, V1Ab and V2A1, respectively, of other teleosts. The V2R has a sequence identity of 70-76% with V2A1 than with the V2B type (sequence identity 43-49%). Semiquantitative PCR analysis showed that the receptor gene transcripts were expressed ubiquitously in the tissues examined (brain, pituitary, gonads, liver, muscle, kidney and gills) and displayed sex and seasonal fluctuations in a tissue-specific manner. The results form a basis for functional studies on the VT receptors in the catfish.

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“…In birds, stress induces the release of adrenocorticotropic hormone (ACTH) from corticotrophs in the anterior pituitary gland. 1 ACTH acts on the adrenal cortex to cause secretion of corticosterone, which affects many physiological processes as a part of the stress response. ACTH release is regulated by the hypothalamic hormones corticotrophin releasing hormone (CRH) and either arginine vasopressin (AVP) in mammals or arginine vasotocin (AVT) in nonmammalian vertebrates.…”

Section: Introductionmentioning

confidence: 99%

Transmembrane domain IV of the Gallus gallus VT[sub 2] vasotocin receptor is essential for forming a heterodimer with the corticotrophin releasing hormone receptor

et al. 2008

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Corticotropin releasing hormone receptor (CRHR) and the VT 2 arginine vasotocin receptor (VT2R) are vital links in the hypothalamic-pituitary-adrenal axis that enable a biological response to stressful stimuli in avian species. CRHR and VT2R are both G-protein coupled receptors (GPCRs), and have been shown by us to form a heterodimer via fluorescent resonance energy transfer (FRET) analysis in the presence of their respective ligands, corticotrophin releasing hormone (CRH) and arginine vasotocin (AVT). The dimerization interface of the heterodimer is unknown, but computational analyses predict transmembrane domains (TMs) as likely sites of the interaction. We constructed chimerical VT2Rs, tagged at the C-terminal ends with either cyan fluorescent protein (CFP) or yellow fluorescent protein (YFP), by replacing the fourth transmembrane region (TM4) of VT2R with TM4 of the β 2 -adrenergic receptor (β 2 AR). The VT2R/β 2 AR chimeras were expressed in HeLa cells and proper trafficking is confirmed by observing cell membrane localization using confocal microscopy. VT2R/β 2 AR-YFP chimera functionality was confirmed with a Fura-2 acetoxymethyl ester (Fura-2AM) assay. FRET analysis was then performed on VT2 / β 2 AR-chimera/CRHR pairs, and the calculated distance was observed to be >10 nm apart, indicating that heterodimerization was partly disrupted by mutating TM4 of the VT2R. Therefore, TM4 may form one region of the possible dimerization interfaces between the VT2R and CRHR.

show abstract

The cloned avian neurohypophysial hormone receptors

Cited by 45 publications

References 62 publications

The oxytocin/vasopressin receptor family has at least five members in the gnathostome lineage, inclucing two distinct V2 subtypes

The oxytocin/vasopressin receptor family has at least five members in the gnathostome lineage, inclucing two distinct V2 subtypes

Molecular cloning, sequencing and phylogeny of vasotocin receptor genes in the air-breathing catfish Heteropneustes fossilis with sex dimorphic and seasonal variations in tissue expression

Transmembrane domain IV of the Gallus gallus VT[sub 2] vasotocin receptor is essential for forming a heterodimer with the corticotrophin releasing hormone receptor

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