The coaction of tonic and phasic dopamine dynamics

Atcherley, Christopher W.; Wood, Kevin M.; Parent, Kate L.; Hashemi, Parastoo; Heien, Michael L.

doi:10.1039/c4cc06165a

Cited by 81 publications

(118 citation statements)

References 43 publications

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“…This technique provides a limit of detection ( < 10 nM dopamine) sufficient for in vivo use and can be alternated with normal FSCV measurements to monitor rapid dopamine changes at the same microelectrode. An initial study employing FSCAV in the mouse striatum reported basal dopamine levels to be close to 100 nM (98), a value consistent with the concentration range suggested by past studies (8, 91–94). …”

Section: Novel Applicationssupporting

confidence: 83%

Electrochemical Analysis of Neurotransmitters

Bucher¹,

Wightman

2015

Annual Rev. Anal. Chem.

251

229

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Chemical signaling through the release of neurotransmitters into the extracellular space is the primary means of communication between neurons. More than four decades ago, Ralph Adams and his colleagues realized the utility of electrochemical methods for the study of easily oxidizable neurotransmitters, such as dopamine, norepinephrine, and serotonin and their metabolites. Today, electrochemical techniques are frequently coupled to microelectrodes to enable spatially resolved recordings of rapid neurotransmitter dynamics in a variety of biological preparations spanning from single cells to the intact brain of behaving animals. In this review, we provide a basic overview of the principles underlying constant-potential amperometry and fast-scan cyclic voltammetry, the most commonly employed electrochemical techniques, and the general application of these methods to the study of neurotransmission. We thereafter discuss several recent developments in sensor design and experimental methodology that are challenging the current limitations defining the application of electrochemical methods to neurotransmitter measurements.

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Section: Novel Applicationssupporting

confidence: 83%

Electrochemical Analysis of Neurotransmitters

Bucher¹,

Wightman

2015

Annual Rev. Anal. Chem.

251

229

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“…18,25–32 Figure 3 shows CVs collected in vitro from these different species at concentrations that mimic a range of reported or predicted physiological values. 4,5,16,24,29,33–39 …”

Section: Resultsmentioning

confidence: 99%

“…15 Our studies revealed a fundamental coaction between evoked and ambient dopamine. 16 Here, we orient this method toward serotonin.…”

mentioning

confidence: 99%

In Vivo Ambient Serotonin Measurements at Carbon-Fiber Microelectrodes

et al. 2017

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The mechanisms that control extracellular serotonin levels in vivo are not well-defined. This shortcoming makes it very challenging to diagnose and treat the many psychiatric disorders in which serotonin is implicated. Fast-scan cyclic voltammetry (FSCV) can measure rapid serotonin release and reuptake events but cannot report critically important ambient serotonin levels. In this Article, we use fast-scan controlled adsorption voltammetry (FSCAV), to measure serotonin’s steady-state, extracellular chemistry. We characterize the “Jackson” voltammetric waveform for FSCAV and show highly stable, selective, and sensitive ambient serotonin measurements in vitro. In vivo, we report basal serotonin levels in the CA2 region of the hippocampus as 64.9 ± 2.3 nM (n = 15 mice, weighted average ± standard error). We electrochemically and pharmacologically verify the selectivity of the serotonin signal. Finally, we develop a statistical model that incorporates the uncertainty in in vivo measurements, in addition to electrode variability, to more critically analyze the time course of pharmacological data. Our novel method is a uniquely powerful analysis tool that can provide deeper insights into the mechanisms that control serotonin’s extracellular levels.

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“…Recent studies have exploited the catecholamine adsorption properties of carbon fiber microelectrodes (CFM), to estimate basal concentrations of neurochemicals. [11,12,13,14].…”

Section: Introductionmentioning

confidence: 99%

Integral methods for automatic quantification of fast-scan-cyclic-voltammetry detected neurotransmitters

Espín

Anders²,

Trevathan³

et al. 2020

Preprint

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Modern techniques for estimating basal levels of electroactive neurotransmitters rely on the measurement of oxidative charges. This requires time integration of oxidation currents at certain intervals. Unfortunately, the selection of integration intervals relies on ad-hoc visual identification of peaks on the oxidation currents, which introduces sources of error and precludes the development of automated procedures necessary for analysis and quantification of neurotransmitter levels in large data sets. In an effort to improve charge quantification techniques, here we present * These authors contributed equally to this work. † Email address: lujan.luis@mayo.edu novel methods for automatic selection of integration boundaries. Our results show that these methods allow quantification of oxidation and reduction reactions, for multiple analytes, both in vitro and in vivo.

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The coaction of tonic and phasic dopamine dynamics

Cited by 81 publications

References 43 publications

Electrochemical Analysis of Neurotransmitters

Electrochemical Analysis of Neurotransmitters

In Vivo Ambient Serotonin Measurements at Carbon-Fiber Microelectrodes

Integral methods for automatic quantification of fast-scan-cyclic-voltammetry detected neurotransmitters

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