The cold shock family gene cspD3 is involved in the pathogenicity of Ralstonia solanacearum CQPS-1 to tobacco

Liu, Ying; Tan, Xi; Cheng, Haojin; Gong, Jie; Zhang, Yong; Wang, Daibin; Ding, Wei

doi:10.1016/j.micpath.2020.104091

Cited by 10 publications

(9 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among the four types of MGEs examined in this study, genes encoding cold shock proteins were only observed in the genomic islands of eight strains isolated from AMD (5) and soil (3), and ICE of two AMD strains. Cold shock domain family proteins are involved in the regulation of multiple phenotypes, including virulence (Michaux et al ., 2017; Liu et al ., 2020), cellular aggregations (Eshwar et al ., 2017), and biofilm (Ray et al ., 2020). Consequently, genomic islands possessing such genes could not only enhance species low temperature resistance at the individual level but also at the population‐level through cellular aggregation or biofilm formation that provide micro‐environments, which would be important for Alicyclobacillus species in adaptation to the harsh AMD environment by creating habitable microenvironments.…”

Section: Discussionmentioning

confidence: 99%

Mobile genetic elements mediate the mixotrophic evolution of novel Alicyclobacillus species for acid mine drainage adaptation

Liu

Liang-gang

Zhou

et al. 2021

Environmental Microbiology

View full text Add to dashboard Cite

Alicyclobacillus species inhabit diverse environments and have adapted to broad ranges of pH and temperature. However, their adaptive evolutions remain elusive, especially regarding the role of mobile genetic elements (MGEs). Here, we characterized the distributions and functions of MGEs in Alicyclobacillus species across five environments, including acid mine drainage (AMD), beverages, hot springs, sediments, and soils. Nine Alicyclobacillus strains were isolated from AMD and possessed larger genome sizes and more genes than those from other environments. Four AMD strains evolved to be mixotrophic and fell into distinctive clusters in phylogenetic tree. Four types of MGEs including genomic island (GI), insertion sequence (IS), prophage, and integrative and conjugative element (ICE) were widely distributed in Alicyclobacillus species. Further, AMD strains did not possess CRISPR-Cas systems, but had more GI, IS, and ICE, as well as more MGE-associated genes involved in the oxidation of iron and sulfide and the resistance of heavy metal and low temperature. These findings highlight the differences in phenotypes and genotypes between strains isolated from AMD and other environments and the important role of MGEs in rapid environment niche expansions.

show abstract

Section: Discussionmentioning

confidence: 99%

Mobile genetic elements mediate the mixotrophic evolution of novel Alicyclobacillus species for acid mine drainage adaptation

Liu

Liang-gang

Zhou

et al. 2021

Environmental Microbiology

View full text Add to dashboard Cite

show abstract

“…Individual CSPs can have minimal effects on bacterial survival when knocked out, although they can affect pathogen virulence. 22,27,28,49 Some MAMP-encoded genes have multiple epitopes which can be recognized by different receptors. 50,51,52 Different epitopes of FliC can be detected from distinct PRRs in Arabidopsis, tomato, rice, and vertebrates.…”

Section: Predictable Arms Race Between Epitope Variation and Protein ...mentioning

confidence: 99%

Natural variation of immune epitopes reveals intrabacterial antagonism

Stevens,

Moreno-Pérez,

Weisberg

et al. 2023

Preprint

View full text Add to dashboard Cite

Plants and animals detect biomolecules termed Microbe-Associated Molecular Patterns (MAMPs) and induce immune responses. The impact of diverse multi-copy MAMPs on immune induction is unknown. We characterized the evolutionary trajectories of five proteinaceous MAMPs and the effect of copy number and sequence variation on plant immune outcomes. From 4,228 plant-associated bacterial genomes, 34,262 MAMPs were identified. Natural variation was constricted, enabling characterization of immune perception across thousands of bacteria. We characterized the immunogenic outcomes of two MAMPs with different evolutionary trajectories the Elongation Factor Tu (EF-Tu) epitope elf18 and Cold Shock Protein (CSP) epitope csp22. EF-Tu is a single-copy gene and 90% of elf18 epitopes are immunogenic. Conversely, CSPs are multi-copy with 99.8% of bacteria carrying at least one immunogenic CSP and only 54% of csp22 epitopes are immunogenic. We uncovered a new mechanism for immune evasion, intrabacterial antagonism, from a non-immunogenic actinobacterial CSP that blocks perception of immunogenic forms.

show abstract

“…The successful mutant was verified by PCR. The complementation analysis was performed with a pUC18-mini-Tn7T-Gm-based site-specific chromosomal integration system as previously described (Zhang et al, 2018;Liu et al, 2020). The primers used in this study are listed in Supplementary Table 1.…”

Section: Construction Of Deletion Mutants and Complementation Analysesmentioning

confidence: 99%

“…A swimming motility test was performed as previously reported (Liu et al, 2020). Briefly, overnight-cultured bacterial suspension (diluted to 1 × 10 8 CFU•ml −1 ) was stab-inoculated into MM medium semi agar (0.3%) plates (MSA medium) supplemented with 20 mM L-glutamate.…”

Section: Swimming Motilitymentioning

confidence: 99%

Mutation in phcA Enhanced the Adaptation of Ralstonia solanacearum to Long-Term Acid Stress

et al. 2022

Self Cite

View full text Add to dashboard Cite

Bacterial wilt, caused by the plant pathogen Ralstonia solanacearum, occurs more severely in acidified soil according to previous reports. However, R. solanacearum cannot grow well in acidic environments under barren nutrient culture conditions, especially when the pH is lower than 5. With the worsening acidification of farmland, further determination of how R. solanacearum adapts to the long-term acidic environment is worthwhile. In this study, experimental evolution was applied to evaluate the adaptability and mechanism of the R. solanacearum experimental population responding to long-term acid stress. We chose the CQPS-1 strain as the ancestor, and minimal medium (MM medium) with different pH values as the culture environment to simulate poor soil. After 1500 generations of serial passage experiments in pH 4.9 MM, acid-adapted experimental strains (denoted as C49 strains) were obtained, showing significantly higher growth rates than the growth rates of control experimental strains (serial passage experiment in pH 6.5 MM, denoted as C65 strains). Competition experiments showed that the competitive indices (CIs) of all selected clones from C49 strains were superior to the ancestor in acidic environment competitiveness. Based on the genome variation analysis and functional verification, we confirmed that loss of function in the phcA gene was associated with the acid fitness gain of R. solanacearum, which meant that the inactivation of the PhcA regulator caused by gene mutation mediated the population expansion of R. solanacearum when growing in an acidic stress environment. Moreover, the swimming motility of acid evolution strains and the phcA deletion mutant was significantly enhanced compared to CQPS-1. This work provided evidence for understanding the adaptive strategy of R. solanacearum to the long-term acidic environment.

show abstract

The cold shock family gene cspD3 is involved in the pathogenicity of Ralstonia solanacearum CQPS-1 to tobacco

Cited by 10 publications

References 46 publications

Mobile genetic elements mediate the mixotrophic evolution of novel Alicyclobacillus species for acid mine drainage adaptation

Mobile genetic elements mediate the mixotrophic evolution of novel Alicyclobacillus species for acid mine drainage adaptation

Natural variation of immune epitopes reveals intrabacterial antagonism

Mutation in phcA Enhanced the Adaptation of Ralstonia solanacearum to Long-Term Acid Stress

Contact Info

Product

Resources

About