The combination of Ilexhainanoside D and ilexsaponin A1 reduces liver inflammation and improves intestinal barrier function in mice with high-fat diet-induced non-alcoholic fatty liver disease

Zhao, Wenwen; Meng, Xiao; Yang, Jie; Zhang, Li; Ba, Yinying; Xu, Rongrong; Liu, Zongyang; Zou, Haiyan; Yu, Ping; Wu, Xia; Chen, Xiaoqing

doi:10.1016/j.phymed.2019.153039

Cited by 26 publications

(12 citation statements)

References 25 publications

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“…However, the reduced abundance of Bacteroides , Phascolarctobacterium were recovered after the administration of RPMP ethanol extract ( Figures 6C , 7B ). Desulfovibrio is a gram-negative bacterium that produces endotoxins and has been shown to increase intestinal permeability and production of enterogenic factors (mainly LPS) ( Zhao et al, 2019 ). The increase in Desulfovibrio abundance caused by HFD was effectively reduced after RPMP ethanol extract treatment ( Figure 6C ).…”

Section: Resultsmentioning

confidence: 99%

Polygoni Multiflori Radix Praeparata Ethanol Extract Exerts a Protective Effect Against High-Fat Diet Induced Non-Alcoholic Fatty Liver Disease in Mice by Remodeling Intestinal Microbial Structure and Maintaining Metabolic Homeostasis of Bile Acids

Dai

et al. 2021

Front. Pharmacol.

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In the prescription of Traditional Chinese Medicine for lipid metabolism, Polygoni Multiflori Radix Preparata (ZhiHeShouWu, RPMP) was widely used. In recent years, RPMP ethanol extract has been reported for the treatment of non-alcoholic fatty liver disease (NAFLD). However, the role of RPMP ethanol extract in the treatment of NAFLD has not been fully elucidated. Therefore, we examined the optimal therapeutic dose of RPMP ethanol extracts. Afterward, a mouse model of non-alcoholic fatty liver induced by a high-fat diet (HFD) was treated with RPMP ethanol extract to further evaluate the mechanism of action of RPMP ethanol extract treatment. And the serum lipid metabolism indexes and liver function indexes showed that the RPMP ethanol extract in the 1.35 g/kg dose group exhibited better therapeutic effects than the 2.70 g/kg dose group. Meanwhile, RPMP ethanol extract can regulate the biochemical indicators of serum and liver to normal levels, and effectively reduce liver steatosis and lipid deposition. RPMP ethanol extract treatment restored HFD-induced disruption of the compositional structure of the intestinal microbial (IM) and bile acids (BAs) pools. And restore the reduced expression of intestinal barrier-related genes caused by HFD administration, which also effectively regulates the expression of genes related to the metabolism of BAs in mice. Thus, RPMP ethanol extract can effectively improve the abnormal lipid metabolism and hepatic lipid accumulation caused by HFD, which may be related to the regulation of IM composition, maintenance of intestinal barrier function, and normal cholesterol metabolism in the body.

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Section: Resultsmentioning

confidence: 99%

Polygoni Multiflori Radix Praeparata Ethanol Extract Exerts a Protective Effect Against High-Fat Diet Induced Non-Alcoholic Fatty Liver Disease in Mice by Remodeling Intestinal Microbial Structure and Maintaining Metabolic Homeostasis of Bile Acids

Dai

et al. 2021

Front. Pharmacol.

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“…Desulfovibrio bacteria are capable of producing H 2 S in the mammalian intestinal tract and are usually found in a variety of habitats including soil, intestine and feces of animals, and both salt and fresh water. [45][46][47] In addition, either increased or decreased abundance of Desulfovibrio bacteria was observed in NAFLD animals, [48][49][50] therefore, the exact role of Desulfovibrio bacteria in NAFLD is not clear. Our results revealed Desulfovibrio bacteria were positively correlated with acetic acid levels and negatively correlated with metabolic disorders suggesting Desulfovibrio bacteria were beneficial rather than detrimental bacteria for metabolic disease.…”

Section: Discussionmentioning

confidence: 99%

Desulfovibrio vulgaris, a potent acetic acid-producing bacterium, attenuates nonalcoholic fatty liver disease in mice

et al. 2021

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The emerging evidence supports the use of prebiotics like herb-derived polysaccharides for treating nonalcoholic fatty liver disease (NAFLD) by modulating gut microbiome. The present study was initiated on the microbiota-dependent anti-NAFLD effect of Astragalus polysaccharides (APS) extracted from Astragalus mongholicus Bunge in high-fat diet (HFD)-fed mice. However, the exact mechanisms underlying the beneficial effects of APS on NAFLD formation remain poorly understood.Co-housing experiment was used to assess the microbiota dependent anti-NAFLD effect of APS. Then, targeted metabolomics and metagenomics were adopted for determining short-chain fatty acids (SCFAs) and bacteria that were specifically enriched by APS. Further in vitro experiment was carried out to test the capacity of SCFAs-producing of identified bacterium. Finally, the anti-NAFLD efficacy of identified bacterium was tested in HFD-fed mice.Our results first demonstrated the anti-NAFLD effect of APS in HFD-fed mice and the contribution of gut microbiota. Moreover, our results indicated that SCFAs, predominantly acetic acid were elevated in APS-supplemented mice and ex vivo experiment. Metagenomics revealed that D. vulgaris from Desulfovibrio genus was not only enriched by APS, but also a potent generator of acetic acid, which showed significant anti-NAFLD effects in HFD-fed mice. In addition, D. vulgaris modulated the hepatic gene expression pattern of lipids metabolism, particularly suppressed hepatic fatty acid synthase (FASN) and CD36 protein expression.Our results demonstrate that APS enriched D. vulgaris is effective on attenuating hepatic steatosis possibly through producing acetic acid, and modulation on hepatic lipids metabolism in mice. Further studies are warranted to explore the long-term impacts of D. vulgaris on host metabolism and the underlying mechanism.

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“…Saponins, as characteristic constituents of the genus Lilium (Munafo and Gianfagna 2015), have many pharmacological activities, including anti-depressant activities (Liu et al 2017), antiinflammation (Zhao et al 2019), etc. In addition, it has been reported that total saponins in L. lancifolium and L. brownii can prolong the sleep time of mice caused by pentobarbital sodium (Li et al 2012.…”

Section: Discussionmentioning

confidence: 99%

“…2017 ), anti-inflammation (Zhao et al. 2019 ), etc. In addition, it has been reported that total saponins in L. lancifolium and L. brownii can prolong the sleep time of mice caused by pentobarbital sodium (Li et al.…”

Section: Discussionmentioning

confidence: 99%

Lilium davidii extract alleviates p‑chlorophenylalanine‑induced insomnia in rats through modification of the hypothalamic-related neurotransmitters, melatonin and homeostasis of the hypothalamic-pituitary-adrenal axis

Wang

Lan

et al. 2020

Pharmaceutical Biology

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The combination of Ilexhainanoside D and ilexsaponin A1 reduces liver inflammation and improves intestinal barrier function in mice with high-fat diet-induced non-alcoholic fatty liver disease

Cited by 26 publications

References 25 publications

Polygoni Multiflori Radix Praeparata Ethanol Extract Exerts a Protective Effect Against High-Fat Diet Induced Non-Alcoholic Fatty Liver Disease in Mice by Remodeling Intestinal Microbial Structure and Maintaining Metabolic Homeostasis of Bile Acids

Polygoni Multiflori Radix Praeparata Ethanol Extract Exerts a Protective Effect Against High-Fat Diet Induced Non-Alcoholic Fatty Liver Disease in Mice by Remodeling Intestinal Microbial Structure and Maintaining Metabolic Homeostasis of Bile Acids

Desulfovibrio vulgaris, a potent acetic acid-producing bacterium, attenuates nonalcoholic fatty liver disease in mice

Lilium davidii extract alleviates p‑chlorophenylalanine‑induced insomnia in rats through modification of the hypothalamic-related neurotransmitters, melatonin and homeostasis of the hypothalamic-pituitary-adrenal axis

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